################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 94 CGCG CG^CG AcsI 379 AAATTC [GA]^AATT[CT] AlwI 125 GGATC GGATC(4/5) ApoI 379 AAATTC [GA]^AATT[CT] BbrPI 40 CACGTG CAC^GTG BbsI 18 GAAGAC GAAGAC(2/6) BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BglI 59 GCCGCATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 18 GAAGAC GAAGAC(2/6) BsaAI 40 CACGTG [CT]AC^GT[GA] BseRI 400 GAGGAG GAGGAG(10/8) BsiYI 8 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 8 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 264 GAATGC GAATGC(1/-1) BsrI 283 ACTGG ACTGG(1/-1) BstF5I 5 CATCC GGATG(2/0) BstUI 94 CGCG CG^CG Cac8I 68 GCAAGC GC[ACGT]^[ACGT]GC DraII 424 GGGGCCT [GA]G^G[ACGT]CC[CT] EarI 392 GAAGAG CTCTTC(1/4) EcoO109I 424 GGGGCCT [GA]G^G[ACGT]CC[CT] FokI 5 CATCC GGATG(9/13) HincII 217 GTCAAC GT[CT]^[GA]AC HindII 217 GTCAAC GT[CT]^[GA]AC Ksp632I 392 GAAGAG CTCTTC(1/4) KspI 93 CCGCGG CCGC^GG MaeII 41 ACGT A^CGT MslI 300 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 93 CCGCGG C[AC]G^C[GT]G MvnI 94 CGCG CG^CG NotI 89 GCGGCCGC GC^GGCCGC PmaCI 40 CACGTG CAC^GTG PmlI 40 CACGTG CAC^GTG PshAI 237 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 313 CGATCG CGAT^CG SacII 93 CCGCGG CCGC^GG SapI 392 GAAGAGC GCTCTTC(1/4) SfaNI 114 GCATC GCATC(5/9) TaqI 134 TCGA T^CGA TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 134 TCGA T^CGA XcmI 296 CCATCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BcnI 23 CCCGG CC^[GC]GG 85 CCCGG BsaWI 74 ACCGGA [AT]^CCGG[AT] 122 ACCGGA BstNI 148 CCAGG CC^[AT]GG 435 CCTGG Cfr13I 36 GGGCC G^G[ACGT]CC 425 GGGCC DpnII 126 GATC ^GATC 314 GATC EagI 57 CGGCCG C^GGCCG 90 CGGCCG EclXI 57 CGGCCG C^GGCCG 90 CGGCCG Eco52I 57 CGGCCG C^GGCCG 90 CGGCCG EcoRII 148 CCAGG ^CC[AT]GG 435 CCTGG HphI 261 GGTGA GGTGA(8/7) 117 TCACC MboI 126 GATC ^GATC 314 GATC MseI 187 TTAA T^TAA 410 TTAA MvaI 148 CCAGG CC^[AT]GG 435 CCTGG NciI 23 CCCGG CC^[GC]GG 85 CCCGG NdeII 126 GATC ^GATC 314 GATC Sau3AI 126 GATC ^GATC 314 GATC Sau96I 36 GGGCC G^G[ACGT]CC 425 GGGCC Tru9I 187 TTAA T^TAA 410 TTAA Tsp509I 230 AATT ^AATT 380 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiEI BssKI DdeI DsaI EaeI Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspI MwoI NlaIV ScrFI TseI