(Linear) (Six-base) MAPSORT of: KIEE5158.seq  Check: 6184  from: 1  to: 444



KIEE5158.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     444 
   Size:        369      75
 
ApoI r'AATT_y

Cuts at:      0     379     444 
   Size:        379      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      25     444 
   Size:         25     419
 
BbvCI CC'TCA_GC

Cuts at:      0     398     444 
   Size:        398      46
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65     444 
   Size:         65     379
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     293     444 
   Size:        293     151
 
Bpu10I CC'TnA_GC

Cuts at:      0     398     444 
   Size:        398      46
 
BsaAI yAC'GTr

Cuts at:      0      42     444 
   Size:         42     402
 
BsaWI w'CCGG_w

Cuts at:      0      74     122     444 
   Size:         74      48     322

  Fragments arranged by size: 

                 322      74      48
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     444 
   Size:        415      29
 
BsiEI CG_ry'CG

Cuts at:      0      60      93     316     444 
   Size:         60      33     223     128

  Fragments arranged by size: 

                 223     128      60      33
 
BsmI GAATG_Cn'

Cuts at:      0     270     444 
   Size:        270     174
 
BtgI C'CryG_G

Cuts at:      0      48      54      93     444 
   Size:         48       6      39     351

  Fragments arranged by size: 

                 351      48      39       6
 
EaeI y'GGCC_r

Cuts at:      0      51      57      90     444 
   Size:         51       6      33     354

  Fragments arranged by size: 

                 354      51      33       6
 
EagI C'GGCC_G

Cuts at:      0      57      90     444 
   Size:         57      33     354

  Fragments arranged by size: 

                 354      57      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     387     444 
   Size:        387      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     444 
   Size:        369      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     425     444 
   Size:        425      19
 
HincII GTy'rAC

Cuts at:      0     219     444 
   Size:        219     225
 
MslI CAynn'nnrTG

Cuts at:      0     304     444 
   Size:        304     140
 
MspA1I CmG'CkG

Cuts at:      0      95     444 
   Size:         95     349
 
NotI GC'GGCC_GC

Cuts at:      0      90     444 
   Size:         90     354
 
PmlI CAC'GTG

Cuts at:      0      42     444 
   Size:         42     402
 
PshAI GACnn'nnGTC

Cuts at:      0     241     444 
   Size:        241     203
 
PvuI CG_AT'CG

Cuts at:      0     316     444 
   Size:        316     128
 
SacII CC_GC'GG

Cuts at:      0      96     444 
   Size:         96     348
 
SapI GCTCTTCn'nnn_

Cuts at:      0     387     444 
   Size:        387      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     161     444 
   Size:        161     283
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     303     444 
   Size:        303     141
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BbvCI     BglI     BmrI   Bpu10I    BsaAI
    BsaWI    BseRI    BsiEI     BsmI     BtgI     EaeI     EagI     EarI
  Eco57MI EcoO109I   HincII     MslI   MspA1I     NotI     PmlI    PshAI
     PvuI    SacII     SapI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI