################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoT22I FauI FseI FspI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AfaI 819 GTAC GT^AC AocI 861 CCTCAGG CC^T[ACGT]AGG Asp700I 137 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 598 GGATCC G^GATCC BanI 686 GGCACC G^G[CT][GA]CC BanII 884 GGGCTC G[GA]GC[CT]^C BbvI 334 GCTGC GCAGC(8/12) BclI 95 TGATCA T^GATCA BsgI 708 CTGCAC GTGCAG(16/14) BsmFI 745 GGGAC GGGAC(10/14) BsmI 319 GAATGC GAATGC(1/-1) Bsp1407I 818 TGTACA T^GTACA BspMI 706 ACCTGC ACCTGC(4/8) BsrBI 29 CCGCTC CCGCTC(-3/-3) BsrDI 450 CATTGC GCAATG(2/0) BsrGI 818 TGTACA T^GTACA BssKI 243 CCTGG ^CC[ACGT]GG BstEII 729 GGTTACC G^GT[ACGT]ACC BstNI 243 CCTGG CC^[AT]GG BstPI 729 GGTTACC G^GT[ACGT]ACC BstXI 602 CCATCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 598 GGATCC [GA]^GATC[CT] Bsu36I 861 CCTCAGG CC^T[ACGT]AGG CfoI 672 GCGC GCG^C DraIII 615 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 338 CCATGG C^C[GA][CT]GG Eco81I 861 CCTCAGG CC^T[ACGT]AGG EcoO65I 729 GGTTACC G^GT[ACGT]ACC EcoRI 3 GAATTC G^AATTC EcoRII 243 CCTGG ^CC[AT]GG EcoRV 559 GATATC GAT^ATC FbaI 95 TGATCA T^GATCA HgaI 323 GCGTC GACGC(5/10) HhaI 672 GCGC GCG^C HinP1I 672 GCGC G^CGC MflI 598 GGATCC [GA]^GATC[CT] MspA1I 296 CAGCGG C[AC]G^C[GT]G MvaI 243 CCTGG CC^[AT]GG NcoI 338 CCATGG C^CATGG NspI 227 ACATGC [GA]CATG^[CT] PleI 80 GAGTC GAGTC(4/5) RsaI 819 GTAC GT^AC ScrFI 243 CCTGG CC^[ACGT]GG SfcI 855 CTACAG C^T[GA][CT]AG SspBI 818 TGTACA T^GTACA StuI 695 AGGCCT AGG^CCT TseI 334 GCTGC G^C[AT]GC XhoII 598 GGATCC [GA]^GATC[CT] XmnI 137 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 3 GAATTC [GA]^AATT[CT] 137 GAATTT ApoI 3 GAATTC [GA]^AATT[CT] 137 GAATTT AvaII 496 GGACC G^G[AT]CC 746 GGACC BmyI 685 GGGCAC G[AGT]GC[ACT]^C 884 GGGCTC BsaJI 270 CCTTGG C^C[ACGT][ACGT]GG 338 CCATGG Bsp1286I 685 GGGCAC G[AGT]GC[ACT]^C 884 GGGCTC BspHI 92 TCATGA T^CATGA 921 TCATGA Cfr13I 496 GGACC G^G[ACGT]CC 746 GGACC EcoT14I 270 CCTTGG C^C[AT][AT]GG 338 CCATGG Fnu4HI 298 GCGGC GC^[ACGT]GC 334 GCTGC HphI 828 GGTGA GGTGA(8/7) 656 TCACC ItaI 298 GCGGC GC^[ACGT]GC 334 GCTGC MseI 58 TTAA T^TAA 878 TTAA RcaI 92 TCATGA T^CATGA 921 TCATGA Sau96I 496 GGACC G^G[ACGT]CC 746 GGACC StyI 270 CCTTGG C^C[AT][AT]GG 338 CCATGG TaqI 78 TCGA T^CGA 317 TCGA TfiI 21 GAATC G^A[AT]TC 831 GATTC Tru9I 58 TTAA T^TAA 878 TTAA Tsp45I 217 GTCAC ^GT[GC]AC 613 GTCAC TthHB8I 78 TCGA T^CGA 317 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AluI AlwI BsiYI BslI BsmAI BstF5I DdeI DpnII EarI FokI HaeIII HinfI Ksp632I MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI Tsp509I TspRI