(Circular) (Six-base) MAPSORT of: pFN21AE5432.seq Check: 5262 from: 1 to: 5671 pFN21AE5432 5671 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2720 2720 Size: 5671 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2690 2915 3734 5118 5386 1632 Size: 404 654 225 819 1384 268 1917 Fragments arranged by size: 1917 1384 819 654 404 268 225 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 2831 1166 Size: 1665 4006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2396 1184 Size: 1212 4459 AlwNI CAG_nnn'CTG Cuts at: 3414 4986 3414 Size: 1572 4099 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2008 2142 2980 3206 3358 5467 1493 Size: 515 134 838 226 152 2109 1697 Fragments arranged by size: 2109 1697 838 515 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 BamHI G'GATC_C Cuts at: 2603 3001 3368 2603 Size: 398 367 4906 Fragments arranged by size: 4906 398 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2691 2992 4447 618 Size: 325 217 627 904 301 1455 1842 Fragments arranged by size: 1842 1455 904 627 325 301 217 BanII G_rGCy'C Cuts at: 1391 2893 2990 1391 Size: 1502 97 4072 Fragments arranged by size: 4072 1502 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 2100 1548 Size: 552 5119 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4282 136 Size: 107 122 71 631 605 2610 1525 Fragments arranged by size: 2610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 2694 3732 4888 1790 Size: 904 1038 1156 2573 Fragments arranged by size: 2573 1156 1038 904 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3402 4360 442 Size: 416 53 83 399 24 1985 958 1753 Fragments arranged by size: 1985 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4331 1239 Size: 3092 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 4874 5370 4874 Size: 496 5175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2698 1163 Size: 573 962 4136 Fragments arranged by size: 4136 962 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3732 3817 4888 3732 Size: 85 1071 4515 Fragments arranged by size: 4515 1071 85 BsmI GAATG_Cn' Cuts at: 2330 3182 3275 2330 Size: 852 93 4726 Fragments arranged by size: 4726 852 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2694 2893 2990 3732 3817 4888 1391 Size: 399 904 199 97 742 85 1071 2174 Fragments arranged by size: 2174 1071 904 742 399 199 97 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 2097 2926 3560 5290 2097 Size: 829 634 1730 2478 Fragments arranged by size: 2478 1730 829 634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2720 3026 844 Size: 1876 306 3489 Fragments arranged by size: 3489 1876 306 BsrBI CCG'CTC Cuts at: 1394 1823 2036 3558 1394 Size: 429 213 1522 3507 Fragments arranged by size: 3507 1522 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 2454 4166 4340 65 Size: 1183 1206 1712 174 1396 Fragments arranged by size: 1712 1396 1206 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 2823 96 Size: 2727 2944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 2734 1184 Size: 1550 4121 BstXI CCAn_nnnn'nTGG Cuts at: 2614 2614 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2603 3001 3368 3753 3770 5222 5308 5320 Size: 631 398 367 385 17 1452 86 12 Cuts at: 5320 5666 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 631 398 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2867 2867 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 2343 513 Size: 552 1278 3841 Fragments arranged by size: 3841 1278 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2378 3187 3981 4001 2378 Size: 809 794 20 4048 Fragments arranged by size: 4048 809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DraIII CAC_nnn'GTG Cuts at: 2625 2625 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3118 4007 5636 Size: 54 1425 50 275 18 1288 889 1629 Cuts at: 5636 8 Size: 43 Fragments arranged by size: 1629 1425 1288 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2498 2891 3601 1353 Size: 746 399 393 710 3423 Fragments arranged by size: 3423 746 710 399 393 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2690 2915 3734 4331 5118 5386 Size: 393 404 654 225 819 597 787 268 Cuts at: 5386 1239 Size: 1524 Fragments arranged by size: 1524 819 787 654 597 404 393 268 225 EcoRI G'AATT_C Cuts at: 2008 2980 3358 2008 Size: 972 378 4321 Fragments arranged by size: 4321 972 378 EcoRV GAT'ATC Cuts at: 2566 2566 Size: 5671 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 4818 1678 Size: 3140 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 4312 4344 4386 Size: 32 135 32 699 32 2009 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 2009 699 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3849 Fragments arranged by size: 3849 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 2768 2831 3629 3988 4147 518 Size: 648 1446 156 63 798 359 159 2042 Fragments arranged by size: 2042 1446 798 648 359 159 156 63 MspA1I CmG'CkG Cuts at: 1606 2303 3087 3764 4912 5157 1606 Size: 697 784 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 697 677 245 NcoI C'CATG_G Cuts at: 513 1065 2343 513 Size: 552 1278 3841 Fragments arranged by size: 3841 1278 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2236 3029 4574 1132 Size: 97 1007 793 1545 2229 Fragments arranged by size: 2229 1545 1007 793 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 3023 830 Size: 2193 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2860 3019 4154 4835 5026 826 Size: 219 1815 159 1135 681 191 1471 Fragments arranged by size: 1815 1471 1135 681 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3775 4676 4938 820 Size: 197 926 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 2702 1517 Size: 1185 4486 StyI C'CwwG_G Cuts at: 513 1065 2275 2343 3110 513 Size: 552 1210 68 767 3074 Fragments arranged by size: 3074 1210 767 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2242 3698 3715 3868 4053 4498 325 Size: 1255 662 1456 17 153 185 445 1498 Fragments arranged by size: 1498 1456 1255 662 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3917 5629 Size: 274 80 33 51 1443 846 1094 1712 Cuts at: 5629 96 Size: 138 Fragments arranged by size: 1712 1443 1094 846 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 2146 3800 2146 Size: 1654 4017 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI