################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MaeI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflII 646 CTTAAG C^TTAAG AlwNI 4 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 181 GGGCCC GGGCC^C AseI 934 ATTAAT AT^TAAT AsnI 934 ATTAAT AT^TAAT AvaI 1538 CTCGAG C^[CT]CG[GA]G BfrI 646 CTTAAG C^TTAAG BpmI 728 CTGGAG CTGGAG(16/14) BsaAI 53 TACGTG [CT]AC^GT[GA] BsaBI 21 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1415 ACCGGA [AT]^CCGG[AT] BsgI 1302 CTGCAC GTGCAG(16/14) BsmFI 839 GGGAC GGGAC(10/14) BsoBI 1538 CTCGAG C^[CT]CG[GA]G Bsp1407I 563 TGTACA T^GTACA BsrDI 605 CATTGC GCAATG(2/0) BsrGI 563 TGTACA T^GTACA DpnII 1194 GATC ^GATC DraI 1009 TTTAAA TTT^AAA DraII 181 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 744 CCATGG C^C[GA][CT]GG EcoNI 1493 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 181 GGGCCCC [GA]G^G[ACGT]CC[CT] HapII 1416 CCGG C^CGG HincII 1393 GTTAAC GT[CT]^[GA]AC HindII 1393 GTTAAC GT[CT]^[GA]AC HindIII 924 AAGCTT A^AGCTT HpaI 1393 GTTAAC GTT^AAC HpaII 1416 CCGG C^CGG MamI 21 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 1194 GATC ^GATC MslI 1466 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1416 CCGG C^CGG NcoI 744 CCATGG C^CATGG NdeII 1194 GATC ^GATC PaeR7I 1538 CTCGAG C^TCGAG PshBI 934 ATTAAT AT^TAAT PstI 424 CTGCAG CTGCA^G Sau3AI 1194 GATC ^GATC SfcI 424 CTGCAG C^T[GA][CT]AG SspBI 563 TGTACA T^GTACA XcmI 104 CCAACCCCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1538 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 211 GAGCTC G[AT]GC[AT]^C 1419 GAGCTC BbvI 3 GCAGC GCAGC(8/12) 93 GCAGC BsaI 231 GAGACC GGTCTC(1/5) 989 GGTCTC BsaJI 694 CCAAGG C^C[ACGT][ACGT]GG 744 CCATGG BseRI 1422 CTCCTC GAGGAG(10/8) 889 GAGGAG BsiHKAI 211 GAGCTC G[AT]GC[AT]^C 1419 GAGCTC BssKI 288 CCTGG ^CC[ACGT]GG 797 CCTGG BstEII 357 GGTGACC G^GT[ACGT]ACC 778 GGTCACC BstNI 288 CCTGG CC^[AT]GG 797 CCTGG BstPI 357 GGTGACC G^GT[ACGT]ACC 778 GGTCACC Cac8I 457 GCTGGC GC[ACGT]^[ACGT]GC 1300 GCCTGC Cfr13I 181 GGGCC G^G[ACGT]CC 182 GGCCC EarI 526 GAAGAG CTCTTC(1/4) 717 GAAGAG EcoO65I 357 GGTGACC G^GT[ACGT]ACC 778 GGTCACC EcoRII 288 CCTGG ^CC[AT]GG 797 CCTGG EcoT14I 694 CCAAGG C^C[AT][AT]GG 744 CCATGG Fnu4HI 3 GCAGC GC^[ACGT]GC 93 GCAGC ItaI 3 GCAGC GC^[ACGT]GC 93 GCAGC Ksp632I 526 GAAGAG CTCTTC(1/4) 717 GAAGAG MaeII 54 ACGT A^CGT 1254 ACGT MvaI 288 CCTGG CC^[AT]GG 797 CCTGG SacI 211 GAGCTC GAGCT^C 1419 GAGCTC Sau96I 181 GGGCC G^G[ACGT]CC 182 GGCCC ScrFI 288 CCTGG CC^[ACGT]GG 797 CCTGG StuI 1298 AGGCCT AGG^CCT 1325 AGGCCT StyI 694 CCAAGG C^C[AT][AT]GG 744 CCATGG TseI 3 GCAGC G^C[AT]GC 93 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BanII BbsI BmyI BpuAI BsiYI BslI BsmAI Bsp1286I BsrI BstF5I DdeI EcoRI FokI HaeIII HinfI HphI MaeIII MboII MnlI MseI NlaIII NlaIV PleI RsaI SfaNI SmlI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XmnI