Restriction map for
pFN21AB7790
(Circular) (Six-base) MAPSORT of: pFN21AB7790.seq Check: 2511 from: 1 to: 6256
pFN21AB7790 6256 bp
With 182 enzymes: SgfI *
March 28, 2012 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 2778 3315 3319 4319 5703 5971 1632
Size: 696 450 537 4 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1000 696 537 450 268 4
AflII C'TTAA_G
Cuts at: 820 1017 2651 820
Size: 197 1634 4425
Fragments arranged by size:
4425 1634 197
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3322 1184
Size: 2138 4118
AlwNI CAG_nnn'CTG
Cuts at: 2014 3999 5571 2014
Size: 1985 1572 2699
Fragments arranged by size:
2699 1985 1572
ApaI G_GGCC'C
Cuts at: 2190 2190
Size: 6256
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2329 2519 2605 2851 2976 3029 3133 3565
Size: 836 190 86 246 125 53 104 432
Cuts at: 3565 3791 3943 6052 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 836 432 246 226 190 152
125 104 86 53
AseI AT'TA_AT
Cuts at: 160 1032 2940 4810 160
Size: 872 1908 1870 1606
Fragments arranged by size:
1908 1870 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3543 3581 1870
Size: 73 1600 38 4545
Fragments arranged by size:
4545 1600 73 38
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3577 5032 618
Size: 325 217 627 1790 1455 1842
Fragments arranged by size:
1842 1790 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2220 3428 3575 1391
Size: 799 30 1208 147 4072
Fragments arranged by size:
4072 1208 799 147 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2711 3354 928
Size: 971 317 495 643 3830
Fragments arranged by size:
3830 971 643 495 317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4447 4481 1903
Size: 34 50 34 2426 34 3678
Fragments arranged by size:
3678 2426 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2190 4317 5473 1790
Size: 400 2127 1156 2573
Fragments arranged by size:
2573 2127 1156 400
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3447 3447
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2754 4916 1239
Size: 1515 2162 2579
Fragments arranged by size:
2579 2162 1515
Bpu10I CC'TnA_GC
Cuts at: 2201 2201
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3106 4381 5246 5544 3106
Size: 1275 865 298 3818
Fragments arranged by size:
3818 1275 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2230 3000 4919 882
Size: 617 731 770 1919 2219
Fragments arranged by size:
2219 1919 770 731 617
BsaAI yAC'GTr
Cuts at: 493 1204 2060 4047 493
Size: 711 856 1987 2702
Fragments arranged by size:
2702 1987 856 711
BsaBI GATnn'nnATC
Cuts at: 2030 6250 2030
Size: 4220 2036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3420 4689 5087 5361 5508 5919 3420
Size: 1269 398 274 147 411 3757
Fragments arranged by size:
3757 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3410 3440 1178
Size: 30 165 30 2007 30 3994
Fragments arranged by size:
3994 2007 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2909 3418 1186
Size: 1723 509 4024
Fragments arranged by size:
4024 1723 509
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3292 1163
Size: 573 1556 4127
Fragments arranged by size:
4127 1556 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3428 4317 4402 5473 2220
Size: 1208 889 85 1071 3003
Fragments arranged by size:
3003 1208 1071 889 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2220 3428 3575 4317 4402 5473
Size: 399 400 30 1208 147 742 85 1071
Cuts at: 5473 1391
Size: 2174
Fragments arranged by size:
2174 1208 1071 742 400 399 147 85
30
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3611 844
Size: 2767 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2609 4751 4925 65
Size: 1183 1361 2142 174 1396
Fragments arranged by size:
2142 1396 1361 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4900 5087 1700
Size: 110 3090 187 2869
Fragments arranged by size:
3090 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2568 96
Size: 2472 3784
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2362 2783 1184
Size: 1178 421 4657
Fragments arranged by size:
4657 1178 421
BstYI r'GATC_y
Cuts at: 1972 3586 3953 4338 4355 5807 5893 5905 6251
Size: 1614 367 385 17 1452 86 12 346
Cuts at: 6251 1972
Size: 1977
Fragments arranged by size:
1977 1614 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2749 513
Size: 552 1684 4020
Fragments arranged by size:
4020 1684 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 3772 4566 4586 3238
Size: 534 794 20 4908
Fragments arranged by size:
4908 794 534 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3304 3339 529
Size: 35 2740 35 3446
Fragments arranged by size:
3446 2740 35 35
DraI TTT'AAA
Cuts at: 3016 3560 3016
Size: 544 5712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3703 4592 6221
Size: 54 1425 50 275 18 1873 889 1629
Cuts at: 6221 8
Size: 43
Fragments arranged by size:
1873 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 2717 4186 1353
Size: 1173 191 1469 3423
Fragments arranged by size:
3423 1469 1173 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 3426 2218
Size: 1208 5048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2754 2778 3315 3319 4319 4916
Size: 393 696 426 24 537 4 1000 597
Cuts at: 4916 5703 5971 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1000 787 696 597 537 426 393
268 24 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3502 3502
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2187 2187
Size: 6256
EcoRI G'AATT_C
Cuts at: 2519 2605 2976 3133 3565 3943 2519
Size: 86 371 157 432 378 4832
Fragments arranged by size:
4832 432 378 371 157 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 5403 1678
Size: 3725 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3183 3215 4897 4929 4971
Size: 32 135 32 1611 32 1682 32 42
Cuts at: 4971 5003 1373
Size: 32 2626
Fragments arranged by size:
2626 1682 1611 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3400 3600 3846 669
Size: 988 281 1462 200 246 3079
Fragments arranged by size:
3079 1462 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 2929 748
Size: 2181 4075
HpaI GTT'AAC
Cuts at: 3400 3846 3400
Size: 446 5810
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 3386 5370 5554 1305
Size: 1332 749 1984 184 2007
Fragments arranged by size:
2007 1984 1332 749 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3475 4214 4573 4732 518
Size: 648 2309 739 359 159 2042
Fragments arranged by size:
2309 2042 739 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3672 4349 5497 5742 1606
Size: 2066 677 1148 245 2120
Fragments arranged by size:
2120 2066 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2749 513
Size: 552 1684 4020
Fragments arranged by size:
4020 1684 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 3614 5159 1132
Size: 97 2385 1545 2229
Fragments arranged by size:
2385 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2186 2186
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2433 3608 830
Size: 1603 1175 3478
Fragments arranged by size:
3478 1603 1175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SacI G_AGCT'C
Cuts at: 2220 3428 2220
Size: 1208 5048
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 2429 3604 4739 5420 5611 826
Size: 219 1384 1175 1135 681 191 1471
Fragments arranged by size:
1471 1384 1175 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3085 3543 4360 5261 5523
Size: 197 926 708 434 458 817 901 262
Cuts at: 5523 820
Size: 1553
Fragments arranged by size:
1553 926 901 817 708 458 434 262
197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3305 3332 1517
Size: 1788 27 4441
Fragments arranged by size:
4441 1788 27
StyI C'CwwG_G
Cuts at: 513 1065 2699 2749 3695 513
Size: 552 1634 50 946 3074
Fragments arranged by size:
3074 1634 946 552 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2314 4283 4300 4453 4638 5083 325
Size: 1255 734 1969 17 153 185 445 1498
Fragments arranged by size:
1969 1498 1255 734 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2568 3095 4502
Size: 274 80 33 51 1443 591 527 1407
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1407 591 527 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2116
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 3543 1943
Size: 1600 4656
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2980 3164 3470 4385 2980
Size: 184 306 915 4851
Fragments arranged by size:
4851 915 306 184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI