################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BclI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] AfaI 180 GTAC GT^AC AlwNI 936 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTT [GA]^AATT[CT] Asp700I 525 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 723 GTGCTC G[AT]GC[AT]^C BalI 374 TGGCCA TGG^CCA BanI 511 GGCACC G^G[CT][GA]CC BbvCI 903 CCTCAGC CCTCAGC(-5/-2) BciVI 944 GTATCC GTATCC(6/5) BglI 427 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 590 AGATCT A^GATCT BpmI 208 CTGGAG CTGGAG(16/14) BsaAI 720 TACGTG [CT]AC^GT[GA] BseRI 726 CTCCTC GAGGAG(10/8) BsiHKAI 723 GTGCTC G[AT]GC[AT]^C BsmAI 745 GTCTC GTCTC(1/5) BstEII 144 GGTTACC G^GT[ACGT]ACC BstPI 144 GGTTACC G^GT[ACGT]ACC DsaI 353 CCATGG C^C[GA][CT]GG EaeI 374 TGGCCA [CT]^GGCC[GA] EcoO65I 144 GGTTACC G^GT[ACGT]ACC FauI 546 CCCGC CCCGC(4/6) HinfI 846 GACTC G^A[ACGT]TC MaeII 721 ACGT A^CGT MluNI 374 TGGCCA TGG^CCA MscI 374 TGGCCA TGG^CCA NcoI 353 CCATGG C^CATGG NdeI 359 CATATG CA^TATG PflMI 502 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 846 GACTC GAGTC(4/5) PstI 381 CTGCAG CTGCA^G RsaI 180 GTAC GT^AC Van91I 502 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 525 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 366 CCGC CCGC(-3/-1) 547 CCGC AlwI 627 GATCC GGATC(4/5) 684 GATCC BmyI 235 GTGCCC G[AGT]GC[ACT]^C 723 GTGCTC BsgI 475 GTGCAG GTGCAG(16/14) 569 GTGCAG BsmFI 250 GGGAC GGGAC(10/14) 386 GTCCC BsmI 334 GAATGC GAATGC(1/-1) 897 GAATGC Bsp1286I 235 GTGCCC G[AGT]GC[ACT]^C 723 GTGCTC BsrDI 77 CATTGC GCAATG(2/0) 663 CATTGC BstXI 239 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 611 CCATTCTGGTGG BstYI 590 AGATCT [GA]^GATC[CT] 683 AGATCC EcoT14I 285 CCTTGG C^C[AT][AT]GG 353 CCATGG MflI 590 AGATCT [GA]^GATC[CT] 683 AGATCC SapI 81 GCTCTTC GCTCTTC(1/4) 489 GCTCTTC SmlI 679 CTCAAG C^T[CT][GA]AG 876 CTTGAG StyI 285 CCTTGG C^C[AT][AT]GG 353 CCATGG Tsp45I 539 GTGAC ^GT[GC]AC 844 GTGAC Tsp509I 32 AATT ^AATT 799 AATT TspRI 581 CACTG CA[GC]TG[ACGT][ACGT]^ 837 CACTG XhoII 590 AGATCT [GA]^GATC[CT] 683 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AvaII BbvI BfaI BsaJI BsiYI BslI BspHI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PpuMI RcaI Sau3AI Sau96I ScrFI SfcI TseI