################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 369 CCGC CCGC(-3/-1) AlwI 71 GGATC GGATC(4/5) AvaI 407 CCCGAG C^[CT]CG[GA]G BalI 311 TGGCCA TGG^CCA BclI 147 TGATCA T^GATCA BsaWI 68 ACCGGA [AT]^CCGG[AT] BsiYI 490 CCATATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 490 CCATATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 715 GTCTC GTCTC(1/5) BsmI 392 GCATTC GAATGC(1/-1) BsoBI 407 CCCGAG C^[CT]CG[GA]G BstXI 230 CCACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 71 GGATCT [GA]^GATC[CT] EaeI 311 TGGCCA [CT]^GGCC[GA] EarI 125 CTCTTC CTCTTC(1/4) EcoRV 562 GATATC GAT^ATC EcoT14I 204 CCTTGG C^C[AT][AT]GG FbaI 147 TGATCA T^GATCA HapII 69 CCGG C^CGG HgaI 676 GCGTC GACGC(5/10) HpaII 69 CCGG C^CGG HphI 287 TCACC GGTGA(8/7) Ksp632I 125 CTCTTC CTCTTC(1/4) MaeII 568 ACGT A^CGT MboII 126 TCTTC GAAGA(8/7) MflI 71 GGATCT [GA]^GATC[CT] MluNI 311 TGGCCA TGG^CCA MscI 311 TGGCCA TGG^CCA MseI 572 TTAA T^TAA MspI 69 CCGG C^CGG NspI 789 ACATGC [GA]CATG^[CT] PflMI 490 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SmlI 655 CTCAAG C^T[CT][GA]AG StyI 204 CCTTGG C^C[AT][AT]GG Tru9I 572 TTAA T^TAA Van91I 490 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 71 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaII 201 GGACC G^G[AT]CC 804 GGTCC BanII 48 GGGCTC G[GA]GC[CT]^C 163 GAGCCC BbvI 249 GCTGC GCAGC(8/12) 277 GCTGC BfaI 253 CTAG C^TAG 736 CTAG BsaJI 204 CCTTGG C^C[ACGT][ACGT]GG 237 CCTGGG BsmFI 759 GGGAC GGGAC(10/14) 805 GTCCC BsrDI 360 CATTGC GCAATG(2/0) 672 CATTGC BssKI 180 CCTGG ^CC[ACGT]GG 237 CCTGG BstF5I 366 CATCC GGATG(2/0) 209 GGATG BstNI 180 CCTGG CC^[AT]GG 237 CCTGG Cac8I 19 GCTGGC GC[ACGT]^[ACGT]GC 189 GCTGGC DdeI 51 CTCAG C^T[ACGT]AG 379 CTGAG DpnII 72 GATC ^GATC 148 GATC EcoRII 180 CCTGG ^CC[AT]GG 237 CCTGG Fnu4HI 249 GCTGC GC^[ACGT]GC 277 GCTGC FokI 366 CATCC GGATG(9/13) 209 GGATG ItaI 249 GCTGC GC^[ACGT]GC 277 GCTGC MaeI 253 CTAG C^TAG 736 CTAG MboI 72 GATC ^GATC 148 GATC MvaI 180 CCTGG CC^[AT]GG 237 CCTGG NdeII 72 GATC ^GATC 148 GATC Sau3AI 72 GATC ^GATC 148 GATC ScrFI 180 CCTGG CC^[ACGT]GG 237 CCTGG SfaNI 619 GATGC GCATC(5/9) 869 GCATC TseI 249 GCTGC G^C[AT]GC 277 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AfaI AluI BmyI Bsp1286I BsrI Cfr13I HaeIII HinfI MnlI MslI MwoI NlaIII NlaIV RsaI Sau96I TfiI Tsp509I TspRI