Restriction map for
pFN21AE5564
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbeI BciVI BfrI BglII BlnI
BsaWI BseAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MroI MseI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tru9I Tsp509I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 715 GACGTC GACGT^C
AccI 349 GTCTAC GT^[AC][GT]AC
AhdI 1139 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 725 GGATC GGATC(4/5)
AspEI 1139 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 8 GAGCTC G[AT]GC[AT]^C
AspI 1261 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1192 CACGTG CAC^GTG
BbvCI 588 CCTCAGC CCTCAGC(-5/-2)
BcgI 3 GCAGGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1058 TGATCA T^GATCA
BfaI 58 CTAG C^TAG
BlpI 891 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 891 GCTGAGC GC^T[ACGT]AGC
BsaBI 411 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 522 GGTCTC GGTCTC(1/5)
BsiHKAI 8 GAGCTC G[AT]GC[AT]^C
BsiWI 530 CGTACG C^GTACG
Bsp1407I 1113 TGTACA T^GTACA
BsrBI 442 GAGCGG CCGCTC(-3/-3)
BsrDI 174 CATTGC GCAATG(2/0)
BsrGI 1113 TGTACA T^GTACA
BstAPI 390 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 725 GGATCT [GA]^GATC[CT]
CelII 891 GCTGAGC GC^T[ACGT]AGC
EagI 320 CGGCCG C^GGCCG
Eam1105I 1139 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 320 CGGCCG C^GGCCG
Eco52I 320 CGGCCG C^GGCCG
EcoNI 732 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1058 TGATCA T^GATCA
KspI 449 CCGCGG CCGC^GG
MaeI 58 CTAG C^TAG
MamI 411 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 725 GGATCT [GA]^GATC[CT]
NaeI 461 GCCGGC GCC^GGC
NgoMIV 461 GCCGGC G^CCGGC
NotI 319 GCGGCCGC GC^GGCCGC
NspI 1052 GCATGC [GA]CATG^[CT]
PflFI 1261 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 488 GAGTC GAGTC(4/5)
PmaCI 1192 CACGTG CAC^GTG
PmlI 1192 CACGTG CAC^GTG
PshAI 516 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 211 CTGCAG CTGCA^G
SacI 8 GAGCTC GAGCT^C
SacII 449 CCGCGG CCGC^GG
SfaNI 602 GCATC GCATC(5/9)
SfcI 211 CTGCAG C^T[GA][CT]AG
SfiI 460 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1052 GCATGC GCATG^C
SspBI 1113 TGTACA T^GTACA
TaqI 677 TCGA T^CGA
Tth111I 1261 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 677 TCGA T^CGA
XcmI 785 CCATCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 725 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Aor51HI 52 AGCGCT AGC^GCT
381 AGCGCT
BalI 566 TGGCCA TGG^CCA
959 TGGCCA
BanI 432 GGCACC G^G[CT][GA]CC
1002 GGTGCC
BglI 461 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
550 GCCATCCTGGC
BsaAI 88 TACGTG [CT]AC^GT[GA]
1192 CACGTG
BsgI 1026 CTGCAC GTGCAG(16/14)
1195 GTGCAG
BsiEI 320 CGGCCG CG[GA][CT]^CG
1145 CGGTCG
BstEII 864 GGTGACC G^GT[ACGT]ACC
1041 GGTCACC
BstPI 864 GGTGACC G^GT[ACGT]ACC
1041 GGTCACC
BstXI 569 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
929 CCAGCGTGCTGG
DdeI 589 CTCAG C^T[ACGT]AG
892 CTGAG
DpnII 726 GATC ^GATC
1059 GATC
Eco47III 52 AGCGCT AGC^GCT
381 AGCGCT
EcoO65I 864 GGTGACC G^GT[ACGT]ACC
1041 GGTCACC
MaeIII 865 GTGAC ^GT[ACGT]AC
1042 GTCAC
MboI 726 GATC ^GATC
1059 GATC
MluNI 566 TGGCCA TGG^CCA
959 TGGCCA
MscI 566 TGGCCA TGG^CCA
959 TGGCCA
NcoI 794 CCATGG C^CATGG
962 CCATGG
NdeII 726 GATC ^GATC
1059 GATC
PflMI 870 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCATGGCCTGG
PpuMI 514 GGGACCC [GA]G^G[AT]CC[CT]
1137 GGGACCC
SapI 357 GCTCTTC GCTCTTC(1/4)
765 GCTCTTC
Sau3AI 726 GATC ^GATC
1059 GATC
SmaI 343 CCCGGG CCC^GGG
662 CCCGGG
TfiI 169 GATTC G^A[AT]TC
537 GATTC
Tsp45I 865 GTGAC ^GT[GC]AC
1042 GTCAC
Van91I 870 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCATGGCCTGG
XmaCI 343 CCCGGG C^CCGGG
662 CCCGGG
XmaI 343 CCCGGG C^CCGGG
662 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PvuII
RsaI Sau96I ScrFI StyI TseI
TspRI