(Circular) (Six-base) MAPSORT of: pFN21AE5564.seq Check: 8386 from: 1 to: 5977 pFN21AE5564 5977 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2612 2612 Size: 5977 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2724 3787 278 Size: 53 83 186 936 1188 1063 2468 Fragments arranged by size: 2468 1188 1063 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 3320 1937 Size: 418 965 4594 Fragments arranged by size: 4594 965 418 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 4040 5424 5692 1632 Size: 1076 1332 1384 268 1917 Fragments arranged by size: 1917 1384 1332 1076 268 AfeI AGC'GCT Cuts at: 2059 2388 2059 Size: 329 5648 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 3149 4706 3149 Size: 1557 4420 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2224 2881 3030 3720 5292 2224 Size: 657 149 690 1572 2909 Fragments arranged by size: 2909 1572 690 657 149 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2348 2441 2667 2725 3302 1870 Size: 73 73 332 93 226 58 577 4545 Fragments arranged by size: 4545 577 332 226 93 73 73 58 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2437 3007 3298 4753 618 Size: 325 217 627 650 570 291 1455 1842 Fragments arranged by size: 1842 1455 650 627 570 325 291 217 BanII G_rGCy'C Cuts at: 1391 2017 2023 2772 2859 3296 1391 Size: 626 6 749 87 437 4072 Fragments arranged by size: 4072 749 626 437 87 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2938 2980 3181 928 Size: 971 1039 42 201 3724 Fragments arranged by size: 3724 1039 971 201 42 BbvCI CC'TCA_GC Cuts at: 2594 2594 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 1997 2021 2031 4168 4202 1903 Size: 34 50 10 24 10 2137 34 3678 Fragments arranged by size: 3678 2137 50 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 3063 1548 Size: 1515 4462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2472 2561 4588 Size: 107 122 71 631 605 800 89 2027 Cuts at: 4588 136 Size: 1525 Fragments arranged by size: 2027 1525 800 631 605 122 107 89 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2897 3394 2897 Size: 497 5480 Bme1580I G_kGCm'C Cuts at: 1790 2552 3012 4038 5194 1790 Size: 762 460 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 762 460 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2174 3708 4666 Size: 416 53 83 399 24 757 1534 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1534 958 757 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2516 2609 2516 Size: 93 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 2837 2846 3259 4637 1239 Size: 1078 520 9 413 1378 2579 Fragments arranged by size: 2579 1378 1078 520 413 9 Bpu10I CC'TnA_GC Cuts at: 2594 2594 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2533 4640 882 Size: 617 1034 2107 2219 Fragments arranged by size: 2219 2107 1034 617 BsaAI yAC'GTr Cuts at: 493 1204 2095 3199 3768 493 Size: 711 891 1104 569 2702 Fragments arranged by size: 2702 1104 891 711 569 BsaBI GATnn'nnATC Cuts at: 2420 5971 2420 Size: 3551 2426 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2487 2640 2721 3180 Size: 53 83 186 936 954 153 81 459 Cuts at: 3180 3784 4166 4801 275 Size: 604 382 635 1451 Fragments arranged by size: 1451 954 936 635 604 459 382 186 153 83 81 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3114 3144 1178 Size: 30 165 30 1711 30 4011 Fragments arranged by size: 4011 1711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 2849 2930 1186 Size: 1273 390 81 4233 Fragments arranged by size: 4233 1273 390 81 BseYI C'CCAG_C Cuts at: 2224 2470 2512 3100 5180 5676 2224 Size: 246 42 588 2080 496 2525 Fragments arranged by size: 2525 2080 588 496 246 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3016 3221 1163 Size: 573 1280 205 3919 Fragments arranged by size: 3919 1280 573 205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2328 3153 3427 4188 5216 1540 Size: 275 186 327 825 274 761 1028 2301 Fragments arranged by size: 2301 1028 825 761 327 275 274 186 BsiHKAI G_wGCw'C Cuts at: 2017 4038 4123 5194 2017 Size: 2021 85 1071 2800 Fragments arranged by size: 2800 2021 1071 85 BsiWI C'GTAC_G Cuts at: 2535 2535 Size: 5977 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2023 2552 2772 2859 3012 3296 Size: 399 227 6 529 220 87 153 284 Cuts at: 3296 4038 4123 5194 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 529 399 284 227 220 153 87 85 6 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 2202 2235 2322 2367 2612 3193 3332 Size: 1282 76 33 87 45 245 581 139 Cuts at: 3332 844 Size: 3489 Fragments arranged by size: 3489 1282 581 245 139 87 76 45 33 BsrBI CCG'CTC Cuts at: 1394 1823 2449 3864 1394 Size: 429 626 1415 3507 Fragments arranged by size: 3507 1415 626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2178 4472 4646 65 Size: 1183 930 2294 174 1396 Fragments arranged by size: 2294 1396 1183 930 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 2233 2466 4621 4808 1700 Size: 110 257 166 233 2155 187 2869 Fragments arranged by size: 2869 2155 257 233 187 166 110 BsrGI T'GTAC_A Cuts at: 96 3118 96 Size: 3022 2955 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 794 Size: 1607 4370 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 2869 3046 1184 Size: 1685 177 4115 Fragments arranged by size: 4115 1685 177 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2941 2581 Size: 360 5617 BstYI r'GATC_y Cuts at: 1972 2730 3307 3674 4059 4076 5528 5614 5626 Size: 758 577 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 758 577 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2454 2799 2967 3147 513 Size: 552 1389 345 168 180 3343 Fragments arranged by size: 3343 1389 552 345 180 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2680 487 Size: 683 56 1454 3784 Fragments arranged by size: 3784 1454 683 56 BtsI GCAGTG_nn' Cuts at: 2651 3125 3493 4287 4307 2651 Size: 474 368 794 20 4321 Fragments arranged by size: 4321 794 474 368 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2325 2553 Size: 54 1425 50 275 18 270 225 228 Cuts at: 2553 2571 2964 3193 3424 4313 5942 8 Size: 18 393 229 231 889 1629 43 Fragments arranged by size: 1629 1425 889 393 275 270 231 229 228 225 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2325 3424 1537 Size: 275 513 1099 4090 Fragments arranged by size: 4090 1099 513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2369 2777 3907 1353 Size: 776 240 408 1130 3423 Fragments arranged by size: 3423 1130 776 408 240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2015 2015 Size: 5977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2708 2837 2846 3259 4040 4637 Size: 393 685 391 129 9 413 781 597 Cuts at: 4637 5424 5692 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 781 685 597 413 393 391 268 129 9 EcoNI CCTnn'n_nnAGG Cuts at: 2741 2741 Size: 5977 EcoO109I rG'GnC_Cy Cuts at: 2520 2878 3143 2520 Size: 358 265 5354 Fragments arranged by size: 5354 358 265 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2061 2279 2309 2390 5124 1678 Size: 383 218 30 81 2734 2531 Fragments arranged by size: 2734 2531 383 218 81 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 3135 3167 4618 Size: 32 135 32 472 32 1059 32 1451 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1451 1059 472 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2470 2778 5091 5275 1305 Size: 1165 308 2313 184 2007 Fragments arranged by size: 2313 2007 1165 308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 2966 10 Size: 54 1425 343 741 393 3021 Fragments arranged by size: 3021 1425 741 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2780 2798 2954 3935 4294 4453 518 Size: 648 1614 18 156 981 359 159 2042 Fragments arranged by size: 2042 1614 981 648 359 159 156 18 MspA1I CmG'CkG Cuts at: 1606 2221 2456 2815 2896 3393 4070 5218 5463 Size: 615 235 359 81 497 677 1148 245 Cuts at: 5463 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 615 497 359 245 235 81 NaeI GCC'GGC Cuts at: 2468 2468 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 2799 2967 513 Size: 552 1734 168 3523 Fragments arranged by size: 3523 1734 552 168 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 2466 2466 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 2325 3424 2325 Size: 1099 4878 NspI r_CATG'y Cuts at: 1132 1229 3061 3335 4880 1132 Size: 97 1832 274 1545 2229 Fragments arranged by size: 2229 1832 1545 274 97 PasI CC'CwG_GG Cuts at: 1348 2025 2461 2875 1348 Size: 677 436 414 4450 Fragments arranged by size: 4450 677 436 414 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 2973 1108 Size: 1773 92 4112 Fragments arranged by size: 4112 1773 92 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 3199 3199 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2520 3143 2520 Size: 623 5354 PshAI GACnn'nnGTC Cuts at: 1613 2525 1613 Size: 912 5065 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 2220 3329 830 Size: 1390 1109 3478 Fragments arranged by size: 3478 1390 1109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2221 2815 2896 2221 Size: 594 81 5302 Fragments arranged by size: 5302 594 81 SacI G_AGCT'C Cuts at: 2017 2017 Size: 5977 SacII CC_GC'GG Cuts at: 2457 2457 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 2520 3143 2520 Size: 623 5354 SapI GCTCTTCn'nnn_ Cuts at: 2369 2777 2369 Size: 408 5569 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2216 3325 4460 5141 5332 826 Size: 219 1171 1109 1135 681 191 1471 Fragments arranged by size: 1471 1171 1135 1109 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2472 2472 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 2350 2669 3304 1872 Size: 478 319 635 4545 Fragments arranged by size: 4545 635 478 319 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3061 3335 1132 Size: 1929 274 3774 Fragments arranged by size: 3774 1929 274 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2301 2799 2967 3416 513 Size: 552 1236 498 168 449 3074 Fragments arranged by size: 3074 1236 552 498 449 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2065 4004 4021 4174 4359 4804 325 Size: 1255 485 1939 17 153 185 445 1498 Fragments arranged by size: 1939 1498 1255 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3118 4223 5935 Size: 274 80 33 51 1443 1141 1105 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1712 1443 1141 1105 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 3269 1316 Size: 1953 4024 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 2797 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 2348 2667 3302 1870 Size: 478 319 635 4545 Fragments arranged by size: 4545 635 478 319 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2722 3785 276 Size: 53 83 186 936 1188 1063 2468 Fragments arranged by size: 2468 1188 1063 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SexAI SfoI SrfI SwaI