(Linear) (Six-base) MAPSORT of: KIEE5564.seq Check: 6409 from: 1 to: 1272 KIEE5564.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 607 1272 Size: 607 665 AatII G_ACGT'C Cuts at: 0 719 1272 Size: 719 553 AccI GT'mk_AC Cuts at: 0 350 1272 Size: 350 922 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1272 Size: 703 569 AfeI AGC'GCT Cuts at: 0 54 383 1272 Size: 54 329 889 Fragments arranged by size: 889 329 54 AhdI GACnn_n'nnGTC Cuts at: 0 1144 1272 Size: 1144 128 AlwNI CAG_nnn'CTG Cuts at: 0 219 876 1025 1272 Size: 219 657 149 247 Fragments arranged by size: 657 247 219 149 AvaI C'yCGr_G Cuts at: 0 11 343 436 662 720 1272 Size: 11 332 93 226 58 552 Fragments arranged by size: 552 332 226 93 58 11 BanI G'GyrC_C Cuts at: 0 432 1002 1272 Size: 432 570 270 Fragments arranged by size: 570 432 270 BanII G_rGCy'C Cuts at: 0 12 18 767 854 1272 Size: 12 6 749 87 418 Fragments arranged by size: 749 418 87 12 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 933 975 1176 1272 Size: 933 42 201 96 Fragments arranged by size: 933 201 96 42 BbvCI CC'TCA_GC Cuts at: 0 589 1272 Size: 589 683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 26 1272 Size: 26 1246 BclI T'GATC_A Cuts at: 0 1058 1272 Size: 1058 214 BglI GCCn_nnn'nGGC Cuts at: 0 467 556 1272 Size: 467 89 716 Fragments arranged by size: 716 467 89 BlpI GC'TnA_GC Cuts at: 0 892 1272 Size: 892 380 Bme1580I G_kGCm'C Cuts at: 0 547 1007 1272 Size: 547 460 265 Fragments arranged by size: 547 460 265 BmrI ACTGGGnnnn_n' Cuts at: 0 169 1272 Size: 169 1103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 511 604 1272 Size: 511 93 668 Fragments arranged by size: 668 511 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 312 832 841 1254 1272 Size: 312 520 9 413 18 Fragments arranged by size: 520 413 312 18 9 Bpu10I CC'TnA_GC Cuts at: 0 589 1272 Size: 589 683 BsaI GGTCTCn'nnnn_ Cuts at: 0 528 1272 Size: 528 744 BsaAI yAC'GTr Cuts at: 0 90 1194 1272 Size: 90 1104 78 Fragments arranged by size: 1104 90 78 BsaBI GATnn'nnATC Cuts at: 0 415 1272 Size: 415 857 BsaHI Gr'CG_yC Cuts at: 0 482 635 716 1175 1272 Size: 482 153 81 459 97 Fragments arranged by size: 482 459 153 97 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1109 1139 1272 Size: 1109 30 133 Fragments arranged by size: 1109 133 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 454 844 925 1272 Size: 454 390 81 347 Fragments arranged by size: 454 390 347 81 BseYI C'CCAG_C Cuts at: 0 219 465 507 1095 1272 Size: 219 246 42 588 177 Fragments arranged by size: 588 246 219 177 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1011 1216 1272 Size: 1011 205 56 Fragments arranged by size: 1011 205 56 BsiEI CG_ry'CG Cuts at: 0 323 1148 1272 Size: 323 825 124 Fragments arranged by size: 825 323 124 BsiHKAI G_wGCw'C Cuts at: 0 12 1272 Size: 12 1260 BsiWI C'GTAC_G Cuts at: 0 530 1272 Size: 530 742 Bsp1286I G_dGCh'C Cuts at: 0 12 18 547 767 854 1007 1272 Size: 12 6 529 220 87 153 265 Fragments arranged by size: 529 265 220 153 87 12 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 121 197 230 317 362 607 1188 1272 Size: 121 76 33 87 45 245 581 84 Fragments arranged by size: 581 245 121 87 84 76 45 33 BsrBI CCG'CTC Cuts at: 0 444 1272 Size: 444 828 BsrDI GCAATG_nn' Cuts at: 0 173 1272 Size: 173 1099 BsrFI r'CCGG_y Cuts at: 0 62 228 461 1272 Size: 62 166 233 811 Fragments arranged by size: 811 233 166 62 BsrGI T'GTAC_A Cuts at: 0 1113 1272 Size: 1113 159 BstAPI GCAn_nnn'nTGC Cuts at: 0 396 1272 Size: 396 876 BstEII G'GTnAC_C Cuts at: 0 864 1041 1272 Size: 864 177 231 Fragments arranged by size: 864 231 177 BstXI CCAn_nnnn'nTGG Cuts at: 0 576 936 1272 Size: 576 360 336 Fragments arranged by size: 576 360 336 BstYI r'GATC_y Cuts at: 0 725 1272 Size: 725 547 BtgI C'CryG_G Cuts at: 0 449 794 962 1142 1272 Size: 449 345 168 180 130 Fragments arranged by size: 449 345 180 168 130 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 675 1272 Size: 675 597 BtsI GCAGTG_nn' Cuts at: 0 646 1120 1272 Size: 646 474 152 Fragments arranged by size: 646 474 152 EaeI y'GGCC_r Cuts at: 0 95 320 548 566 959 1188 1272 Size: 95 225 228 18 393 229 84 Fragments arranged by size: 393 229 228 225 95 84 18 EagI C'GGCC_G Cuts at: 0 320 1272 Size: 320 952 EarI CTCTTCn'nnn_ Cuts at: 0 124 364 772 1272 Size: 124 240 408 500 Fragments arranged by size: 500 408 240 124 EcoICRI GAG'CTC Cuts at: 0 10 1272 Size: 10 1262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 312 703 832 841 1254 1272 Size: 312 391 129 9 413 18 Fragments arranged by size: 413 391 312 129 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 736 1272 Size: 736 536 EcoO109I rG'GnC_Cy Cuts at: 0 515 873 1138 1272 Size: 515 358 265 134 Fragments arranged by size: 515 358 265 134 HaeII r_GCGC'y Cuts at: 0 56 274 304 385 1272 Size: 56 218 30 81 887 Fragments arranged by size: 887 218 81 56 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 39 71 1130 1162 1272 Size: 39 32 1059 32 110 Fragments arranged by size: 1059 110 39 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 465 773 1272 Size: 465 308 499 Fragments arranged by size: 499 465 308 MscI TGG'CCA Cuts at: 0 568 961 1272 Size: 568 393 311 Fragments arranged by size: 568 393 311 MslI CAynn'nnrTG Cuts at: 0 775 793 949 1272 Size: 775 18 156 323 Fragments arranged by size: 775 323 156 18 MspA1I CmG'CkG Cuts at: 0 216 451 810 891 1272 Size: 216 235 359 81 381 Fragments arranged by size: 381 359 235 216 81 NaeI GCC'GGC Cuts at: 0 463 1272 Size: 463 809 NcoI C'CATG_G Cuts at: 0 794 962 1272 Size: 794 168 310 Fragments arranged by size: 794 310 168 NgoMIV G'CCGG_C Cuts at: 0 461 1272 Size: 461 811 NotI GC'GGCC_GC Cuts at: 0 320 1272 Size: 320 952 NspI r_CATG'y Cuts at: 0 1056 1272 Size: 1056 216 PasI CC'CwG_GG Cuts at: 0 20 456 870 1272 Size: 20 436 414 402 Fragments arranged by size: 436 414 402 20 PflMI CCAn_nnn'nTGG Cuts at: 0 876 968 1272 Size: 876 92 304 Fragments arranged by size: 876 304 92 PmlI CAC'GTG Cuts at: 0 1194 1272 Size: 1194 78 PpuMI rG'GwC_Cy Cuts at: 0 515 1138 1272 Size: 515 623 134 Fragments arranged by size: 623 515 134 PshAI GACnn'nnGTC Cuts at: 0 520 1272 Size: 520 752 PstI C_TGCA'G Cuts at: 0 215 1272 Size: 215 1057 PvuII CAG'CTG Cuts at: 0 216 810 891 1272 Size: 216 594 81 381 Fragments arranged by size: 594 381 216 81 SacI G_AGCT'C Cuts at: 0 12 1272 Size: 12 1260 SacII CC_GC'GG Cuts at: 0 452 1272 Size: 452 820 SanDI GG'GwC_CC Cuts at: 0 515 1138 1272 Size: 515 623 134 Fragments arranged by size: 623 515 134 SapI GCTCTTCn'nnn_ Cuts at: 0 364 772 1272 Size: 364 408 500 Fragments arranged by size: 500 408 364 SfcI C'TryA_G Cuts at: 0 211 1272 Size: 211 1061 SfiI GGCCn_nnn'nGGCC Cuts at: 0 467 1272 Size: 467 805 SmaI CCC'GGG Cuts at: 0 345 664 1272 Size: 345 319 608 Fragments arranged by size: 608 345 319 SphI G_CATG'C Cuts at: 0 1056 1272 Size: 1056 216 StyI C'CwwG_G Cuts at: 0 296 794 962 1272 Size: 296 498 168 310 Fragments arranged by size: 498 310 296 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 60 1272 Size: 60 1212 TatI w'GTAC_w Cuts at: 0 1113 1272 Size: 1113 159 Tth111I GACn'n_nGTC Cuts at: 0 1264 1272 Size: 1264 8 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 792 1272 Size: 792 480 XmaI C'CCGG_G Cuts at: 0 343 662 1272 Size: 343 319 610 Fragments arranged by size: 610 343 319 ZraI GAC'GTC Cuts at: 0 717 1272 Size: 717 555 Enzymes that do cut: AarI AatII AccI AcuI AfeI AhdI AlwNI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PflMI PmlI PpuMI PshAI PstI PvuII SacI SacII SanDI SapI SfcI SfiI SmaI SphI StyI TaqII TatI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BmgBI BmtI BpuEI BsaWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI