################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BseRI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 291 GTAC GT^AC AspHI 322 GAGCAC G[AT]GC[AT]^C AvaI 447 CCCGGG C^[CT]CG[GA]G AviII 335 TGCGCA TGC^GCA BanI 241 GGCGCC G^G[CT][GA]CC BanII 469 GAGCCC G[GA]GC[CT]^C BbeI 241 GGCGCC GGCGC^C BbrPI 226 CACGTG CAC^GTG BbvCI 357 GCTGAGG CCTCAGC(-5/-2) BpmI 558 CTCCAG CTGGAG(16/14) BsaAI 226 CACGTG [CT]AC^GT[GA] BsaWI 133 ACCGGA [AT]^CCGG[AT] BsiHKAI 322 GAGCAC G[AT]GC[AT]^C BsmBI 268 GAGACG CGTCTC(1/5) BsoBI 447 CCCGGG C^[CT]CG[GA]G BsrDI 164 GCAATG GCAATG(2/0) BstF5I 294 CATCC GGATG(2/0) FokI 294 CATCC GGATG(9/13) FspI 335 TGCGCA TGC^GCA HinfI 528 GAGTC G^A[ACGT]TC HphI 195 GGTGA GGTGA(8/7) KasI 241 GGCGCC G^GCGCC MaeII 227 ACGT A^CGT MboII 439 TCTTC GAAGA(8/7) NaeI 104 GCCGGC GCC^GGC NarI 241 GGCGCC GG^CGCC NgoMIV 104 GCCGGC G^CCGGC PleI 528 GAGTC GAGTC(4/5) PmaCI 226 CACGTG CAC^GTG PmlI 226 CACGTG CAC^GTG PshAI 74 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 561 CAGCTG CAG^CTG RsaI 291 GTAC GT^AC SfoI 241 GGCGCC GGC^GCC SmaI 447 CCCGGG CCC^GGG TaqI 551 TCGA T^CGA TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 551 TCGA T^CGA XmaCI 447 CCCGGG C^CCGGG XmaI 447 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 241 GGCGCC G[GA]^CG[CT]C 328 GACGCC AvaII 23 GGTCC G^G[AT]CC 404 GGACC BglI 387 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 452 GCCGCGGGGGC BlpI 255 GCTGAGC GC^T[ACGT]AGC 278 GCTCAGC BmyI 322 GAGCAC G[AGT]GC[ACT]^C 469 GAGCCC Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC 278 GCTCAGC BsaHI 241 GGCGCC G[GA]^CG[CT]C 328 GACGCC BsaI 72 GAGACC GGTCTC(1/5) 130 GAGACC Bsp1286I 322 GAGCAC G[AGT]GC[ACT]^C 469 GAGCCC BsrFI 104 GCCGGC [GA]^CCGG[CT] 117 ACCGGC BssHII 299 GCGCGC G^CGCGC 521 GCGCGC CelII 255 GCTGAGC GC^T[ACGT]AGC 278 GCTCAGC Cfr10I 104 GCCGGC [GA]^CCGG[CT] 117 ACCGGC EagI 149 CGGCCG C^GGCCG 376 CGGCCG EclXI 149 CGGCCG C^GGCCG 376 CGGCCG Eco52I 149 CGGCCG C^GGCCG 376 CGGCCG Hin1I 241 GGCGCC G[GA]^CG[CT]C 328 GACGCC KspI 390 CCGCGG CCGC^GG 453 CCGCGG PstI 99 CTGCAG CTGCA^G 304 CTGCAG SacII 390 CCGCGG CCGC^GG 453 CCGCGG SfcI 99 CTGCAG C^T[GA][CT]AG 304 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AluI AlwNI BbvI BcnI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI