(Circular) (Six-base) MAPSORT of: pFN21AE1679.seq Check: 3050 from: 1 to: 5284 pFN21AE1679 5284 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3094 278 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2627 1937 Size: 690 4594 Acc65I G'GTAC_C Cuts at: 2605 2605 Size: 5284 AclI AA'CG_TT Cuts at: 1563 3411 3784 1563 Size: 1848 373 3063 Fragments arranged by size: 3063 1848 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 4731 4999 1632 Size: 1715 1384 268 1917 Fragments arranged by size: 1917 1715 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5087 AflIII A'CryG_T Cuts at: 1203 4183 5160 1203 Size: 2980 977 1327 Fragments arranged by size: 2980 1327 977 AgeI A'CCGG_T Cuts at: 4115 4115 Size: 5284 AhdI GACnn_n'nnGTC Cuts at: 4013 4013 Size: 5284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5284 AlwNI CAG_nnn'CTG Cuts at: 2221 2317 2517 3027 4599 2221 Size: 96 200 510 1572 2906 Fragments arranged by size: 2906 1572 510 200 96 ApaLI G'TGCA_C Cuts at: 3341 4497 3341 Size: 1156 4128 ApoI r'AATT_y Cuts at: 1493 2593 2819 2971 5080 1493 Size: 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 226 152 AseI AT'TA_AT Cuts at: 160 1032 3838 160 Size: 872 2806 1606 Fragments arranged by size: 2806 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 AvaI C'yCGr_G Cuts at: 1870 1943 2452 2609 1870 Size: 73 509 157 4545 Fragments arranged by size: 4545 509 157 73 BamHI G'GATC_C Cuts at: 2614 2981 2614 Size: 367 4917 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2605 4060 618 Size: 325 217 627 459 359 1455 1842 Fragments arranged by size: 1842 1455 627 459 359 325 217 BanII G_rGCy'C Cuts at: 1391 2478 2603 1391 Size: 1087 125 4072 Fragments arranged by size: 4072 1087 125 BbeI G_GCGC'C Cuts at: 2250 2250 Size: 5284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4313 BbvCI CC'TCA_GC Cuts at: 2363 2363 Size: 5284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3475 3509 1903 Size: 34 1538 34 3678 Fragments arranged by size: 3678 1538 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3176 4386 3176 Size: 1210 4074 BclI T'GATC_A Cuts at: 1548 1548 Size: 5284 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2398 2463 3895 Size: 107 122 71 631 605 726 65 1432 Cuts at: 3895 136 Size: 1525 Fragments arranged by size: 1525 1432 726 631 605 122 107 71 65 BglII A'GATC_T Cuts at: 5279 5279 Size: 5284 BlpI GC'TnA_GC Cuts at: 2261 2284 2701 2261 Size: 23 417 4844 Fragments arranged by size: 4844 417 23 Bme1580I G_kGCm'C Cuts at: 1790 3345 4501 1790 Size: 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5284 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3015 3973 442 Size: 416 53 83 399 24 1598 958 1753 Fragments arranged by size: 1753 1598 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2497 2497 Size: 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2548 3944 1239 Size: 1309 1396 2579 Fragments arranged by size: 2579 1396 1309 Bpu10I CC'TnA_GC Cuts at: 2363 2363 Size: 5284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3409 4274 4572 3409 Size: 865 298 4121 Fragments arranged by size: 4121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 2129 3947 882 Size: 617 572 58 1818 2219 Fragments arranged by size: 2219 1818 617 572 58 BsaAI yAC'GTr Cuts at: 493 1204 2233 3075 493 Size: 711 1029 842 2702 Fragments arranged by size: 2702 1029 842 711 BsaBI GATnn'nnATC Cuts at: 5278 5278 Size: 5284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2247 2334 3091 3473 Size: 53 83 186 936 714 87 757 382 Cuts at: 3473 4108 275 Size: 635 1451 Fragments arranged by size: 1451 936 757 714 635 382 186 87 83 53 BsaWI w'CCGG_w Cuts at: 2138 3717 4115 4389 4536 4947 2138 Size: 1579 398 274 147 411 2475 Fragments arranged by size: 2475 1579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5059 Fragments arranged by size: 5059 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5284 BseYI C'CCAG_C Cuts at: 4487 4983 4487 Size: 496 4788 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4711 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2123 2157 2384 2421 2734 3495 Size: 275 186 122 34 227 37 313 761 Cuts at: 3495 4523 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 313 275 227 186 122 37 34 BsiHKAI G_wGCw'C Cuts at: 2331 3345 3430 4501 2331 Size: 1014 85 1071 3114 Fragments arranged by size: 3114 1071 1014 85 BsmI GAATG_Cn' Cuts at: 2795 2888 2795 Size: 93 5191 BsmBI CGTCTCn'nnnn_ Cuts at: 2267 2267 Size: 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2478 2603 3345 3430 4501 1391 Size: 399 541 147 125 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 147 125 85 BspEI T'CCGG_A Cuts at: 4947 4947 Size: 5284 BspHI T'CATG_A Cuts at: 3173 4903 3173 Size: 1730 3554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2639 844 Size: 1795 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3171 1394 Size: 429 1348 3507 Fragments arranged by size: 3507 1348 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 3779 3953 65 Size: 1183 928 1603 174 1396 Fragments arranged by size: 1603 1396 1183 928 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 2122 3928 4115 1700 Size: 110 299 13 1806 187 2869 Fragments arranged by size: 2869 1806 299 187 110 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5284 BssHII G'CGCG_C Cuts at: 1925 2304 2526 1925 Size: 379 222 4683 Fragments arranged by size: 4683 379 222 BssSI C'ACGA_G Cuts at: 3344 4356 3344 Size: 1012 4272 BstAPI GCAn_nnn'nTGC Cuts at: 794 2233 2317 2377 794 Size: 1439 84 60 3701 Fragments arranged by size: 3701 1439 84 60 BstBI TT'CG_AA Cuts at: 4088 4088 Size: 5284 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5284 BstYI r'GATC_y Cuts at: 1972 2614 2981 3366 3383 4835 4921 4933 5279 Size: 642 367 385 17 1452 86 12 346 Cuts at: 5279 1972 Size: 1977 Fragments arranged by size: 1977 1452 642 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2289 2395 2458 513 Size: 552 939 285 106 63 3339 Fragments arranged by size: 3339 939 552 285 106 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4545 Fragments arranged by size: 4545 683 56 BtsI GCAGTG_nn' Cuts at: 2018 2800 3594 3614 2018 Size: 782 794 20 3688 Fragments arranged by size: 3688 794 782 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5249 DraI TTT'AAA Cuts at: 2588 2588 Size: 5284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4291 809 Size: 507 2975 1802 Fragments arranged by size: 2975 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2154 2322 Size: 54 1425 50 275 18 294 30 168 Cuts at: 2322 2381 2731 3620 5249 8 Size: 59 350 889 1629 43 Fragments arranged by size: 1629 1425 889 350 294 275 168 59 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2154 2381 2731 1537 Size: 275 342 227 350 4090 Fragments arranged by size: 4090 350 342 275 227 EarI CTCTTCn'nnn_ Cuts at: 1353 3214 1353 Size: 1861 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2089 2482 3871 4245 4391 387 Size: 1064 138 500 393 1389 374 146 1280 Fragments arranged by size: 1389 1280 1064 500 393 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2548 3347 3944 4731 4999 1239 Size: 393 916 799 597 787 268 1524 Fragments arranged by size: 1524 916 799 787 597 393 268 EcoRI G'AATT_C Cuts at: 2593 2971 2593 Size: 378 4906 FspI TGC'GCA Cuts at: 2342 3790 2342 Size: 1448 3836 HaeII r_GCGC'y Cuts at: 1678 2222 2250 2317 4431 1678 Size: 544 28 67 2114 2531 Fragments arranged by size: 2531 2114 544 67 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3925 3957 3999 4031 1373 Size: 32 135 32 2353 32 42 32 2626 Fragments arranged by size: 2626 2353 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2874 669 Size: 988 281 690 246 3079 Fragments arranged by size: 3079 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5284 HpaI GTT'AAC Cuts at: 2874 2874 Size: 5284 KasI G'GCGC_C Cuts at: 2246 2246 Size: 5284 KpnI G_GTAC'C Cuts at: 2609 2609 Size: 5284 MfeI C'AATT_G Cuts at: 2883 2883 Size: 5284 MluI A'CGCG_T Cuts at: 5160 5160 Size: 5284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2484 4398 4582 1305 Size: 1179 1914 184 2007 Fragments arranged by size: 2007 1914 1179 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3462 Fragments arranged by size: 3462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 3601 3760 518 Size: 648 2076 359 159 2042 Fragments arranged by size: 2076 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2153 2397 2460 2514 2568 2700 3377 4525 Size: 547 244 63 54 54 132 677 1148 Cuts at: 4525 4770 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 547 245 244 132 63 54 54 NaeI GCC'GGC Cuts at: 2111 2111 Size: 5284 NarI GG'CG_CC Cuts at: 2247 2247 Size: 5284 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3793 Fragments arranged by size: 3793 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5284 NgoMIV G'CCGG_C Cuts at: 2109 2109 Size: 5284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5284 NotI GC'GGCC_GC Cuts at: 2731 2731 Size: 5284 NspI r_CATG'y Cuts at: 1132 1229 2642 4187 1132 Size: 97 1413 1545 2229 Fragments arranged by size: 2229 1545 1413 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5284 PciI A'CATG_T Cuts at: 4183 4183 Size: 5284 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5284 PmeI GTTT'AAAC Cuts at: 2588 2588 Size: 5284 PmlI CAC'GTG Cuts at: 2233 2233 Size: 5284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3385 4866 4898 3353 Size: 32 1481 32 3739 Fragments arranged by size: 3739 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2083 1613 Size: 470 4814 PsiI TTA'TAA Cuts at: 2854 2854 Size: 5284 PstI C_TGCA'G Cuts at: 830 2108 2313 2636 830 Size: 1278 205 323 3478 Fragments arranged by size: 3478 1278 323 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5284 PvuII CAG'CTG Cuts at: 2568 2568 Size: 5284 SacII CC_GC'GG Cuts at: 2398 2461 2398 Size: 63 5221 SalI G'TCGA_C Cuts at: 1936 2626 1936 Size: 690 4594 SbfI CC_TGCA'GG Cuts at: 2636 2636 Size: 5284 ScaI AGT'ACT Cuts at: 3532 5244 3532 Size: 1712 3572 SfcI C'TryA_G Cuts at: 826 1045 2104 2309 2632 3767 4448 4639 826 Size: 219 1059 205 323 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 681 323 219 205 191 SfoI GGC'GCC Cuts at: 2248 2248 Size: 5284 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5284 SmaI CCC'GGG Cuts at: 1872 2454 2611 1872 Size: 582 157 4545 Fragments arranged by size: 4545 582 157 SmlI C'TyrA_G Cuts at: 820 1017 1943 3388 4289 4551 820 Size: 197 926 1445 901 262 1553 Fragments arranged by size: 1553 1445 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3075 493 Size: 2582 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5284 SphI G_CATG'C Cuts at: 1132 2642 1132 Size: 1510 3774 SspI AAT'ATT Cuts at: 5 52 3208 5 Size: 47 3156 2081 Fragments arranged by size: 3156 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5284 StyI C'CwwG_G Cuts at: 513 1065 2004 2723 513 Size: 552 939 719 3074 Fragments arranged by size: 3074 939 719 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 3328 3481 3666 4111 325 Size: 1255 1731 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 3530 5242 Size: 274 80 33 51 1443 318 1235 1712 Cuts at: 5242 96 Size: 138 Fragments arranged by size: 1712 1443 1235 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5244 5276 5244 Size: 32 5252 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5284 XbaI T'CTAG_A Cuts at: 2620 2620 Size: 5284 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5284 XmaI C'CCGG_G Cuts at: 1870 2452 2609 1870 Size: 582 157 4545 Fragments arranged by size: 4545 582 157 XmnI GAAnn'nnTTC Cuts at: 3413 3413 Size: 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3092 276 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI