################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AfaI 36 GTAC GT^AC AlwI 117 GATCC GGATC(4/5) AvaI 488 CCCGGG C^[CT]CG[GA]G AvaII 713 GGACC G^G[AT]CC BalI 449 TGGCCA TGG^CCA BcgI 294 GCAGAGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsmBI 519 GAGACG CGTCTC(1/5) BsoBI 488 CCCGGG C^[CT]CG[GA]G BspDI 538 ATCGAT AT^CGAT BsrI 336 ACTGG ACTGG(1/-1) BstYI 116 AGATCC [GA]^GATC[CT] ClaI 538 ATCGAT AT^CGAT DsaI 422 CCATGG C^C[GA][CT]GG EaeI 449 TGGCCA [CT]^GGCC[GA] EcoNI 447 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 737 GCGGG CCCGC(4/6) MflI 116 AGATCC [GA]^GATC[CT] MluNI 449 TGGCCA TGG^CCA MscI 449 TGGCCA TGG^CCA MslI 544 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 601 CAGCTG C[AC]G^C[GT]G NcoI 422 CCATGG C^CATGG NlaIII 423 CATG CATG^ PpuMI 712 GGGACCC [GA]G^G[AT]CC[CT] PvuII 601 CAGCTG CAG^CTG RsaI 36 GTAC GT^AC SfaNI 370 GATGC GCATC(5/9) SfcI 435 CTACAG C^T[GA][CT]AG SmaI 488 CCCGGG CCC^GGG TspRI 105 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 116 AGATCC [GA]^GATC[CT] XmaCI 488 CCCGGG C^CCGGG XmaI 488 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AspHI 184 GAGCTC G[AT]GC[AT]^C 580 GAGCTC BanII 184 GAGCTC G[GA]GC[CT]^C 580 GAGCTC BbsI 553 GAAGAC GAAGAC(2/6) 417 GTCTTC BbvI 291 GCAGC GCAGC(8/12) 440 GCAGC BmyI 184 GAGCTC G[AGT]GC[ACT]^C 580 GAGCTC BpmI 187 CTCCAG CTGGAG(16/14) 604 CTGGAG BpuAI 553 GAAGAC GAAGAC(2/6) 417 GTCTTC BsaI 93 GAGACC GGTCTC(1/5) 589 GAGACC BsiHKAI 184 GAGCTC G[AT]GC[AT]^C 580 GAGCTC BsmFI 566 GGGAC GGGAC(10/14) 712 GGGAC Bsp1286I 184 GAGCTC G[AGT]GC[ACT]^C 580 GAGCTC BsrBI 529 CCGCTC CCGCTC(-3/-3) 284 GAGCGG BstF5I 150 CATCC GGATG(2/0) 369 GGATG Cac8I 524 GCAAGC GC[ACGT]^[ACGT]GC 737 GCGGGC DpnII 117 GATC ^GATC 541 GATC DraII 191 AGGCCCT [GA]G^G[ACGT]CC[CT] 712 GGGACCC EcoO109I 191 AGGCCCT [GA]G^G[ACGT]CC[CT] 712 GGGACCC FokI 150 CATCC GGATG(9/13) 369 GGATG HapII 219 CCGG C^CGG 489 CCGG HgaI 521 GACGC GACGC(5/10) 162 GCGTC HpaII 219 CCGG C^CGG 489 CCGG MboI 117 GATC ^GATC 541 GATC MspI 219 CCGG C^CGG 489 CCGG MwoI 277 GCAGAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 728 GCAAAGTCGGC NdeII 117 GATC ^GATC 541 GATC SacI 184 GAGCTC GAGCT^C 580 GAGCTC Sau3AI 117 GATC ^GATC 541 GATC TseI 291 GCAGC G^C[AT]GC 440 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BcnI BsaJI BseRI BsgI BsiYI BslI BsmAI BssKI BstNI CfoI Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MboII MnlI MvaI NciI NlaIV Sau96I ScrFI StyI TaqI TfiI TthHB8I