(Circular) (Six-base) MAPSORT of: pFN21AE6639.seq Check: 6884 from: 1 to: 5455 pFN21AE6639 5455 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 1937 Size: 861 4594 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2423 3518 4902 5170 1632 Size: 791 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1095 791 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2553 1166 Size: 1387 4068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2764 2990 3142 5251 1493 Size: 1271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1271 226 152 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2493 2780 1870 Size: 73 550 287 4545 Fragments arranged by size: 4545 550 287 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 4231 618 Size: 325 217 627 989 1455 1842 Fragments arranged by size: 1842 1455 989 627 325 217 BanII G_rGCy'C Cuts at: 1391 2193 2589 2774 1391 Size: 802 396 185 4072 Fragments arranged by size: 4072 802 396 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 2565 928 Size: 971 516 150 3818 Fragments arranged by size: 3818 971 516 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 3646 3680 1903 Size: 34 351 34 1324 34 3678 Fragments arranged by size: 3678 1324 351 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4066 136 Size: 107 122 71 631 605 2394 1525 Fragments arranged by size: 2394 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 3516 4672 1790 Size: 1726 1156 2573 Fragments arranged by size: 2573 1726 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4144 442 Size: 416 53 83 399 24 1769 958 1753 Fragments arranged by size: 1769 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2630 4115 1239 Size: 938 453 1485 2579 Fragments arranged by size: 2579 1485 938 453 Bpu10I CC'TnA_GC Cuts at: 2672 2672 Size: 5455 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3580 4445 4743 3580 Size: 865 298 4292 Fragments arranged by size: 4292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2092 2588 4118 882 Size: 617 593 496 1530 2219 Fragments arranged by size: 2219 1530 617 593 496 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3262 3644 4279 275 Size: 53 83 186 936 1729 382 635 1451 Fragments arranged by size: 1729 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 4579 Fragments arranged by size: 4579 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2102 2249 2252 2255 2381 2384 2398 Size: 862 54 147 3 3 126 3 14 Cuts at: 2398 1186 Size: 4243 Fragments arranged by size: 4243 862 147 126 54 14 3 3 3 BseYI C'CCAG_C Cuts at: 2714 2722 4658 5154 2714 Size: 8 1936 496 3015 Fragments arranged by size: 3015 1936 496 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2301 2331 2585 1163 Size: 573 565 30 254 4033 Fragments arranged by size: 4033 573 565 254 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2589 3516 3601 4672 2193 Size: 396 927 85 1071 2976 Fragments arranged by size: 2976 1071 927 396 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 BsmBI CGTCTCn'nnnn_ Cuts at: 2518 2518 Size: 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2589 2774 3516 3601 4672 1391 Size: 399 403 396 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 396 185 85 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2291 2536 3342 1394 Size: 429 468 245 806 3507 Fragments arranged by size: 3507 806 468 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 3950 4124 65 Size: 1183 2702 174 1396 Fragments arranged by size: 2702 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4099 4286 1700 Size: 110 2289 187 2869 Fragments arranged by size: 2869 2289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2121 2785 3152 3537 3554 5006 5092 5104 Size: 149 664 367 385 17 1452 86 12 Cuts at: 5104 5450 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 664 385 367 346 149 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2427 513 Size: 552 1362 3541 Fragments arranged by size: 3541 1362 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2109 2971 3765 3785 2109 Size: 862 794 20 3779 Fragments arranged by size: 3779 862 794 20 ClaI AT'CG_AT Cuts at: 1554 2544 1554 Size: 990 4465 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 2902 3791 Size: 54 1425 50 275 18 624 448 889 Cuts at: 3791 5420 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 624 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2064 2079 2465 3385 1353 Size: 693 18 15 386 920 3423 Fragments arranged by size: 3423 920 693 386 18 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 4042 4416 4562 387 Size: 1064 138 771 1682 374 146 1280 Fragments arranged by size: 1682 1280 1064 771 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2587 2191 Size: 396 5059 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2423 2630 3518 4115 4902 5170 Size: 393 545 246 207 888 597 787 268 Cuts at: 5170 1239 Size: 1524 Fragments arranged by size: 1524 888 787 597 545 393 268 246 207 EcoNI CCTnn'n_nnAGG Cuts at: 2456 2456 Size: 5455 EcoO109I rG'GnC_Cy Cuts at: 2197 2718 2197 Size: 521 4934 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2278 2332 2749 4602 1678 Size: 600 54 417 1853 2531 Fragments arranged by size: 2531 1853 600 417 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 4096 4128 4170 Size: 32 135 32 519 32 1973 32 42 Cuts at: 4170 4202 1373 Size: 32 2626 Fragments arranged by size: 2626 1973 519 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 10 Size: 54 1425 343 624 3009 Fragments arranged by size: 3009 1425 624 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 3413 3772 3931 518 Size: 648 1387 860 359 159 2042 Fragments arranged by size: 2042 1387 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 2871 3548 4696 4941 1606 Size: 1002 263 677 1148 245 2120 Fragments arranged by size: 2120 1148 1002 677 263 245 NcoI C'CATG_G Cuts at: 513 1065 2427 513 Size: 552 1362 3541 Fragments arranged by size: 3541 1362 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2813 4358 1132 Size: 97 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5455 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2718 2718 Size: 5455 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 PvuII CAG'CTG Cuts at: 2608 2608 Size: 5455 SacI G_AGCT'C Cuts at: 2193 2589 2193 Size: 396 5059 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SanDI GG'GwC_CC Cuts at: 2718 2718 Size: 5455 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2440 2803 3938 4619 4810 826 Size: 219 1395 363 1135 681 191 1471 Fragments arranged by size: 1471 1395 1135 681 363 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2495 2782 1872 Size: 623 287 4545 Fragments arranged by size: 4545 623 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3559 4460 4722 820 Size: 197 926 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2427 2457 2551 2580 2727 2894 513 Size: 552 1362 30 94 29 147 167 3074 Fragments arranged by size: 3074 1362 552 167 147 94 30 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3701 5413 96 Size: 274 80 33 51 1443 1724 1712 138 Fragments arranged by size: 1724 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2493 2780 1870 Size: 623 287 4545 Fragments arranged by size: 4545 623 287 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI