(Linear) (Six-base) MAPSORT of: KIEE6639.seq Check: 656 from: 1 to: 750 KIEE6639.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 750 Size: 418 332 AleI CACnn'nnGTG Cuts at: 0 548 750 Size: 548 202 AvaI C'yCGr_G Cuts at: 0 488 750 Size: 488 262 BanII G_rGCy'C Cuts at: 0 188 584 750 Size: 188 396 166 Fragments arranged by size: 396 188 166 BbsI GAAGACnn'nnnn_ Cuts at: 0 410 560 750 Size: 410 150 190 Fragments arranged by size: 410 190 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 750 Size: 283 34 433 Fragments arranged by size: 433 283 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 625 750 Size: 172 453 125 Fragments arranged by size: 453 172 125 Bpu10I CC'TnA_GC Cuts at: 0 667 750 Size: 667 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 87 583 750 Size: 87 496 167 Fragments arranged by size: 496 167 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 49 750 Size: 19 30 701 Fragments arranged by size: 701 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 97 244 247 250 376 379 393 Size: 43 54 147 3 3 126 3 14 Cuts at: 393 750 Size: 357 Fragments arranged by size: 357 147 126 54 43 14 3 3 3 BseYI C'CCAG_C Cuts at: 0 709 717 750 Size: 709 8 33 Fragments arranged by size: 709 33 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 326 580 750 Size: 296 30 254 170 Fragments arranged by size: 296 254 170 30 BsiHKAI G_wGCw'C Cuts at: 0 188 584 750 Size: 188 396 166 Fragments arranged by size: 396 188 166 BsmBI CGTCTCn'nnnn_ Cuts at: 0 513 750 Size: 513 237 Bsp1286I G_dGCh'C Cuts at: 0 188 584 750 Size: 188 396 166 Fragments arranged by size: 396 188 166 BsrBI CCG'CTC Cuts at: 0 286 531 750 Size: 286 245 219 Fragments arranged by size: 286 245 219 BstYI r'GATC_y Cuts at: 0 116 750 Size: 116 634 BtgI C'CryG_G Cuts at: 0 422 750 Size: 422 328 BtsI GCAGTG_nn' Cuts at: 0 104 750 Size: 104 646 ClaI AT'CG_AT Cuts at: 0 539 750 Size: 539 211 EaeI y'GGCC_r Cuts at: 0 449 750 Size: 449 301 EarI CTCTTCn'nnn_ Cuts at: 0 41 59 74 460 750 Size: 41 18 15 386 290 Fragments arranged by size: 386 290 41 18 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 355 750 Size: 355 395 EcoICRI GAG'CTC Cuts at: 0 186 582 750 Size: 186 396 168 Fragments arranged by size: 396 186 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 418 625 750 Size: 172 246 207 125 Fragments arranged by size: 246 207 172 125 EcoNI CCTnn'n_nnAGG Cuts at: 0 451 750 Size: 451 299 EcoO109I rG'GnC_Cy Cuts at: 0 192 713 750 Size: 192 521 37 Fragments arranged by size: 521 192 37 HaeII r_GCGC'y Cuts at: 0 273 327 744 750 Size: 273 54 417 6 Fragments arranged by size: 417 273 54 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 86 118 750 Size: 86 32 632 Fragments arranged by size: 632 86 32 MscI TGG'CCA Cuts at: 0 451 750 Size: 451 299 MslI CAynn'nnrTG Cuts at: 0 548 750 Size: 548 202 MspA1I CmG'CkG Cuts at: 0 603 750 Size: 603 147 NcoI C'CATG_G Cuts at: 0 422 750 Size: 422 328 PpuMI rG'GwC_Cy Cuts at: 0 713 750 Size: 713 37 PvuII CAG'CTG Cuts at: 0 603 750 Size: 603 147 SacI G_AGCT'C Cuts at: 0 188 584 750 Size: 188 396 166 Fragments arranged by size: 396 188 166 SanDI GG'GwC_CC Cuts at: 0 713 750 Size: 713 37 SfcI C'TryA_G Cuts at: 0 435 750 Size: 435 315 SmaI CCC'GGG Cuts at: 0 490 750 Size: 490 260 StyI C'CwwG_G Cuts at: 0 422 452 546 575 722 750 Size: 422 30 94 29 147 28 Fragments arranged by size: 422 147 94 30 29 28 XmaI C'CCGG_G Cuts at: 0 488 750 Size: 488 262 Enzymes that do cut: AcuI AleI AvaI BanII BbsI BcgI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BstYI BtgI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I NcoI PpuMI PvuII SacI SanDI SfcI SmaI StyI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI