################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 99 CGCG CG^CG AfaI 36 GTAC GT^AC AlwI 141 GATCC GGATC(4/5) AvaI 512 CCCGGG C^[CT]CG[GA]G AvaII 737 GGACC G^G[AT]CC BalI 473 TGGCCA TGG^CCA BcgI 318 GCAGAGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsmBI 543 GAGACG CGTCTC(1/5) BsoBI 512 CCCGGG C^[CT]CG[GA]G BspDI 562 ATCGAT AT^CGAT BsrI 360 ACTGG ACTGG(1/-1) BstUI 99 CGCG CG^CG BstYI 140 AGATCC [GA]^GATC[CT] ClaI 562 ATCGAT AT^CGAT DsaI 446 CCATGG C^C[GA][CT]GG EaeI 473 TGGCCA [CT]^GGCC[GA] EcoNI 471 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 761 GCGGG CCCGC(4/6) MflI 140 AGATCC [GA]^GATC[CT] MluNI 473 TGGCCA TGG^CCA MscI 473 TGGCCA TGG^CCA MslI 568 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 625 CAGCTG C[AC]G^C[GT]G MvnI 99 CGCG CG^CG NcoI 446 CCATGG C^CATGG NlaIII 447 CATG CATG^ PpuMI 736 GGGACCC [GA]G^G[AT]CC[CT] PvuII 625 CAGCTG CAG^CTG RsaI 36 GTAC GT^AC SfaNI 394 GATGC GCATC(5/9) SfcI 459 CTACAG C^T[GA][CT]AG SmaI 512 CCCGGG CCC^GGG TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 140 AGATCC [GA]^GATC[CT] XmaCI 512 CCCGGG C^CCGGG XmaI 512 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 208 GAGCTC G[AT]GC[AT]^C 604 GAGCTC BanII 208 GAGCTC G[GA]GC[CT]^C 604 GAGCTC BbvI 315 GCAGC GCAGC(8/12) 464 GCAGC BmyI 208 GAGCTC G[AGT]GC[ACT]^C 604 GAGCTC BpmI 211 CTCCAG CTGGAG(16/14) 628 CTGGAG BsaI 117 GAGACC GGTCTC(1/5) 613 GAGACC BsiHKAI 208 GAGCTC G[AT]GC[AT]^C 604 GAGCTC BsmFI 590 GGGAC GGGAC(10/14) 736 GGGAC Bsp1286I 208 GAGCTC G[AGT]GC[ACT]^C 604 GAGCTC BsrBI 553 CCGCTC CCGCTC(-3/-3) 308 GAGCGG BstF5I 174 CATCC GGATG(2/0) 393 GGATG Cac8I 548 GCAAGC GC[ACGT]^[ACGT]GC 761 GCGGGC DpnII 141 GATC ^GATC 565 GATC DraII 215 AGGCCCT [GA]G^G[ACGT]CC[CT] 736 GGGACCC EcoO109I 215 AGGCCCT [GA]G^G[ACGT]CC[CT] 736 GGGACCC FokI 174 CATCC GGATG(9/13) 393 GGATG HapII 243 CCGG C^CGG 513 CCGG HpaII 243 CCGG C^CGG 513 CCGG MboI 141 GATC ^GATC 565 GATC MspI 243 CCGG C^CGG 513 CCGG MwoI 301 GCAGAGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 752 GCAAAGTCGGC NdeII 141 GATC ^GATC 565 GATC SacI 208 GAGCTC GAGCT^C 604 GAGCTC Sau3AI 141 GATC ^GATC 565 GATC TseI 315 GCAGC G^C[AT]GC 464 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BbsI BcnI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BssKI BstNI CfoI Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI HaeII HaeIII HgaI HhaI HinP1I HinfI HphI ItaI Ksp632I MboII MnlI MvaI NciI NlaIV Sau96I ScrFI StyI TaqI TfiI TthHB8I