(Linear) (Six-base) MAPSORT of: KIEE2513.seq Check: 2006 from: 1 to: 774 KIEE2513.orf With 182 enzymes: SgfI * February 25, 2014 15:45 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 774 Size: 442 332 AleI CACnn'nnGTG Cuts at: 0 572 774 Size: 572 202 AvaI C'yCGr_G Cuts at: 0 512 774 Size: 512 262 BanII G_rGCy'C Cuts at: 0 212 608 774 Size: 212 396 166 Fragments arranged by size: 396 212 166 BbsI GAAGACnn'nnnn_ Cuts at: 0 86 434 584 774 Size: 86 348 150 190 Fragments arranged by size: 348 190 150 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 307 341 774 Size: 307 34 433 Fragments arranged by size: 433 307 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 649 774 Size: 196 453 125 Fragments arranged by size: 453 196 125 Bpu10I CC'TnA_GC Cuts at: 0 691 774 Size: 691 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 111 607 774 Size: 111 496 167 Fragments arranged by size: 496 167 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 49 774 Size: 19 30 725 Fragments arranged by size: 725 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 106 121 268 271 274 400 403 Size: 43 63 15 147 3 3 126 3 Cuts at: 403 417 774 Size: 14 357 Fragments arranged by size: 357 147 126 63 43 15 14 3 3 3 BseYI C'CCAG_C Cuts at: 0 733 741 774 Size: 733 8 33 Fragments arranged by size: 733 33 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 350 604 774 Size: 320 30 254 170 Fragments arranged by size: 320 254 170 30 BsiHKAI G_wGCw'C Cuts at: 0 212 608 774 Size: 212 396 166 Fragments arranged by size: 396 212 166 BsmBI CGTCTCn'nnnn_ Cuts at: 0 537 774 Size: 537 237 Bsp1286I G_dGCh'C Cuts at: 0 212 608 774 Size: 212 396 166 Fragments arranged by size: 396 212 166 BsrBI CCG'CTC Cuts at: 0 310 555 774 Size: 310 245 219 Fragments arranged by size: 310 245 219 BstYI r'GATC_y Cuts at: 0 140 774 Size: 140 634 BtgI C'CryG_G Cuts at: 0 446 774 Size: 446 328 BtsI GCAGTG_nn' Cuts at: 0 128 774 Size: 128 646 ClaI AT'CG_AT Cuts at: 0 563 774 Size: 563 211 EaeI y'GGCC_r Cuts at: 0 473 774 Size: 473 301 EarI CTCTTCn'nnn_ Cuts at: 0 41 59 98 484 774 Size: 41 18 39 386 290 Fragments arranged by size: 386 290 41 39 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 379 774 Size: 379 395 EcoICRI GAG'CTC Cuts at: 0 210 606 774 Size: 210 396 168 Fragments arranged by size: 396 210 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 442 649 774 Size: 196 246 207 125 Fragments arranged by size: 246 207 196 125 EcoNI CCTnn'n_nnAGG Cuts at: 0 475 774 Size: 475 299 EcoO109I rG'GnC_Cy Cuts at: 0 216 737 774 Size: 216 521 37 Fragments arranged by size: 521 216 37 HaeII r_GCGC'y Cuts at: 0 297 351 768 774 Size: 297 54 417 6 Fragments arranged by size: 417 297 54 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 110 142 774 Size: 110 32 632 Fragments arranged by size: 632 110 32 MscI TGG'CCA Cuts at: 0 475 774 Size: 475 299 MslI CAynn'nnrTG Cuts at: 0 572 774 Size: 572 202 MspA1I CmG'CkG Cuts at: 0 627 774 Size: 627 147 NcoI C'CATG_G Cuts at: 0 446 774 Size: 446 328 PpuMI rG'GwC_Cy Cuts at: 0 737 774 Size: 737 37 PvuII CAG'CTG Cuts at: 0 627 774 Size: 627 147 SacI G_AGCT'C Cuts at: 0 212 608 774 Size: 212 396 166 Fragments arranged by size: 396 212 166 SanDI GG'GwC_CC Cuts at: 0 737 774 Size: 737 37 SfcI C'TryA_G Cuts at: 0 459 774 Size: 459 315 SmaI CCC'GGG Cuts at: 0 514 774 Size: 514 260 StyI C'CwwG_G Cuts at: 0 446 476 570 599 746 774 Size: 446 30 94 29 147 28 Fragments arranged by size: 446 147 94 30 29 28 XmaI C'CCGG_G Cuts at: 0 512 774 Size: 512 262 Enzymes that do cut: AcuI AleI AvaI BanII BbsI BcgI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BstYI BtgI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I NcoI PpuMI PvuII SacI SanDI SfcI SmaI StyI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI