(Linear) (Six-base) MAPSORT of: KIEE5532.seq Check: 4085 from: 1 to: 1179 KIEE5532.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1080 1179 Size: 1080 99 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 994 1099 1179 Size: 358 636 105 80 Fragments arranged by size: 636 358 105 80 AhdI GACnn_n'nnGTC Cuts at: 0 861 1179 Size: 861 318 AlwNI CAG_nnn'CTG Cuts at: 0 200 852 972 1155 1179 Size: 200 652 120 183 24 Fragments arranged by size: 652 200 183 120 24 AvaI C'yCGr_G Cuts at: 0 215 697 1109 1179 Size: 215 482 412 70 Fragments arranged by size: 482 412 215 70 BanII G_rGCy'C Cuts at: 0 896 1179 Size: 896 283 BbsI GAAGACnn'nnnn_ Cuts at: 0 1056 1179 Size: 1056 123 BglII A'GATC_T Cuts at: 0 314 1179 Size: 314 865 BlpI GC'TnA_GC Cuts at: 0 517 592 1179 Size: 517 75 587 Fragments arranged by size: 587 517 75 BmgBI CAC'GTC Cuts at: 0 346 384 1059 1179 Size: 346 38 675 120 Fragments arranged by size: 675 346 120 38 BmrI ACTGGGnnnn_n' Cuts at: 0 253 1179 Size: 253 926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 1120 1179 Size: 124 996 59 Fragments arranged by size: 996 124 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 1179 Size: 205 974 BsaI GGTCTCn'nnnn_ Cuts at: 0 1134 1179 Size: 1134 45 BsaWI w'CCGG_w Cuts at: 0 479 1179 Size: 479 700 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 907 937 1179 Size: 907 30 242 Fragments arranged by size: 907 242 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 526 1179 Size: 10 516 653 Fragments arranged by size: 653 516 10 BseYI C'CCAG_C Cuts at: 0 232 711 1116 1143 1179 Size: 232 479 405 27 36 Fragments arranged by size: 479 405 232 36 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 631 1179 Size: 415 216 548 Fragments arranged by size: 548 415 216 BsiHKAI G_wGCw'C Cuts at: 0 1022 1179 Size: 1022 157 BsmBI CGTCTCn'nnnn_ Cuts at: 0 336 1179 Size: 336 843 Bsp1286I G_dGCh'C Cuts at: 0 896 1022 1179 Size: 896 126 157 Fragments arranged by size: 896 157 126 BspEI T'CCGG_A Cuts at: 0 479 1179 Size: 479 700 BspHI T'CATG_A Cuts at: 0 773 1179 Size: 773 406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1080 1179 Size: 1080 99 BsrFI r'CCGG_y Cuts at: 0 129 1179 Size: 129 1050 BstAPI GCAn_nnn'nTGC Cuts at: 0 200 438 1179 Size: 200 238 741 Fragments arranged by size: 741 238 200 BstYI r'GATC_y Cuts at: 0 314 1179 Size: 314 865 BtgI C'CryG_G Cuts at: 0 259 270 527 1179 Size: 259 11 257 652 Fragments arranged by size: 652 259 257 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 175 1179 Size: 175 1004 BtsI GCAGTG_nn' Cuts at: 0 1053 1179 Size: 1053 126 DraIII CAC_nnn'GTG Cuts at: 0 384 438 1179 Size: 384 54 741 Fragments arranged by size: 741 384 54 EaeI y'GGCC_r Cuts at: 0 348 635 1179 Size: 348 287 544 Fragments arranged by size: 544 348 287 EarI CTCTTCn'nnn_ Cuts at: 0 16 103 334 1033 1179 Size: 16 87 231 699 146 Fragments arranged by size: 699 231 146 87 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 358 994 1099 1120 1179 Size: 124 234 636 105 21 59 Fragments arranged by size: 636 234 124 105 59 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 454 1179 Size: 454 725 EcoO109I rG'GnC_Cy Cuts at: 0 426 849 986 1152 1179 Size: 426 423 137 166 27 Fragments arranged by size: 426 423 166 137 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 827 847 859 879 1179 Size: 827 20 12 20 300 Fragments arranged by size: 827 300 20 20 12 MslI CAynn'nnrTG Cuts at: 0 557 619 1179 Size: 557 62 560 Fragments arranged by size: 560 557 62 MspA1I CmG'CkG Cuts at: 0 336 588 711 744 1179 Size: 336 252 123 33 435 Fragments arranged by size: 435 336 252 123 33 NcoI C'CATG_G Cuts at: 0 259 270 1179 Size: 259 11 909 Fragments arranged by size: 909 259 11 NspI r_CATG'y Cuts at: 0 421 624 720 1179 Size: 421 203 96 459 Fragments arranged by size: 459 421 203 96 PflMI CCAn_nnn'nTGG Cuts at: 0 384 852 1155 1179 Size: 384 468 303 24 Fragments arranged by size: 468 384 303 24 PfoI T'CCnGG_A Cuts at: 0 851 1179 Size: 851 328 PpuMI rG'GwC_Cy Cuts at: 0 849 1179 Size: 849 330 PshAI GACnn'nnGTC Cuts at: 0 529 1179 Size: 529 650 PstI C_TGCA'G Cuts at: 0 43 335 1091 1179 Size: 43 292 756 88 Fragments arranged by size: 756 292 88 43 PvuII CAG'CTG Cuts at: 0 336 588 711 744 1179 Size: 336 252 123 33 435 Fragments arranged by size: 435 336 252 123 33 SapI GCTCTTCn'nnn_ Cuts at: 0 1033 1179 Size: 1033 146 SbfI CC_TGCA'GG Cuts at: 0 1091 1179 Size: 1091 88 SfcI C'TryA_G Cuts at: 0 39 331 791 1087 1179 Size: 39 292 460 296 92 Fragments arranged by size: 460 296 292 92 39 SmaI CCC'GGG Cuts at: 0 217 699 1111 1179 Size: 217 482 412 68 Fragments arranged by size: 482 412 217 68 SmlI C'TyrA_G Cuts at: 0 184 1179 Size: 184 995 SphI G_CATG'C Cuts at: 0 421 1179 Size: 421 758 StuI AGG'CCT Cuts at: 0 1012 1179 Size: 1012 167 StyI C'CwwG_G Cuts at: 0 259 270 287 390 422 926 1179 Size: 259 11 17 103 32 504 253 Fragments arranged by size: 504 259 253 103 32 17 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 643 718 1179 Size: 643 75 461 Fragments arranged by size: 643 461 75 TatI w'GTAC_w Cuts at: 0 240 1031 1179 Size: 240 791 148 Fragments arranged by size: 791 240 148 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 379 411 1179 Size: 379 32 768 Fragments arranged by size: 768 379 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 556 1179 Size: 556 623 XmaI C'CCGG_G Cuts at: 0 215 697 1109 1179 Size: 215 482 412 70 Fragments arranged by size: 482 412 215 70 Enzymes that do cut: AarI AcuI AhdI AlwNI AvaI BanII BbsI BglII BlpI BmgBI BmrI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BstAPI BstYI BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MslI MspA1I NcoI NspI PflMI PfoI PpuMI PshAI PstI PvuII SapI SbfI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI