################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MaeI MaeIII MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 492 GGTACC G^GTACC Alw44I 630 GTGCAC G^TGCAC AlwI 648 GATCC GGATC(4/5) AlwNI 646 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 630 GTGCAC G^TGCAC Asp718I 492 GGTACC G^GTACC AspHI 630 GTGCAC G[AT]GC[AT]^C BalI 194 TGGCCA TGG^CCA BcnI 544 CCCGG CC^[GC]GG BmyI 630 GTGCAC G[AGT]GC[ACT]^C BsiHKAI 630 GTGCAC G[AT]GC[AT]^C BsmFI 147 GTCCC GGGAC(10/14) Bsp1286I 630 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 476 TGTACA T^GTACA BsrFI 178 ACCGGC [GA]^CCGG[CT] BsrGI 476 TGTACA T^GTACA BsrI 464 ACTGG ACTGG(1/-1) BstYI 647 AGATCC [GA]^GATC[CT] Cfr10I 178 ACCGGC [GA]^CCGG[CT] DpnII 648 GATC ^GATC DraIII 549 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 282 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 489 GCGGG CCCGC(4/6) HgaI 446 GCGTC GACGC(5/10) HinfI 499 GACTC G^A[ACGT]TC KpnI 492 GGTACC GGTAC^C MaeII 392 ACGT A^CGT MboI 648 GATC ^GATC MflI 647 AGATCC [GA]^GATC[CT] MluNI 194 TGGCCA TGG^CCA MscI 194 TGGCCA TGG^CCA MslI 669 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 435 CCGCTG C[AC]G^C[GT]G NciI 544 CCCGG CC^[GC]GG NdeII 648 GATC ^GATC PflMI 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 499 GACTC GAGTC(4/5) SapI 54 GCTCTTC GCTCTTC(1/4) Sau3AI 648 GATC ^GATC SexAI 550 ACCTGGT A^CC[AT]GGT SgrAI 177 CACCGGCG C[GA]^CCGG[CT]G SspBI 476 TGTACA T^GTACA TaqI 470 TCGA T^CGA TthHB8I 470 TCGA T^CGA Van91I 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 197 CCATCGGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 647 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 118 AGCT AG^CT 526 AGCT BbeI 181 GGCGCC GGCGC^C 561 GGCGCC BsgI 535 CTGCAC GTGCAG(16/14) 643 GTGCAG BsmAI 574 GAGAC GTCTC(1/5) 448 GTCTC BsmBI 447 CGTCTC CGTCTC(1/5) 574 GAGACG BssHII 4 GCGCGC G^CGCGC 6 GCGCGC BstF5I 303 CATCC GGATG(2/0) 420 CATCC Cfr13I 396 GGGCC G^G[ACGT]CC 654 GGGCC EaeI 101 TGGCCG [CT]^GGCC[GA] 194 TGGCCA FokI 303 CATCC GGATG(9/13) 420 CATCC HapII 179 CCGG C^CGG 545 CCGG HpaII 179 CCGG C^CGG 545 CCGG KasI 181 GGCGCC G^GCGCC 561 GGCGCC MspI 179 CCGG C^CGG 545 CCGG NarI 181 GGCGCC GG^CGCC 561 GGCGCC Sau96I 396 GGGCC G^G[ACGT]CC 654 GGGCC SfoI 181 GGCGCC GGC^GCC 561 GGCGCC TspRI 459 CACTG CA[GC]TG[ACGT][ACGT]^ 519 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AcyI AfaI BanI BbvI BpmI BsaHI BsaJI BsiYI BslI BspMI BssKI BstNI BstUI Cac8I CfoI EarI EcoRII Fnu4HI HaeII HaeIII HhaI Hin1I HinP1I HphI ItaI Ksp632I MboII MnlI MvaI MvnI MwoI NlaIII NlaIV RsaI ScrFI SfaNI TseI