Restriction map for
pFN21AE1730
(Circular) (Six-base) MAPSORT of: pFN21AE1730.seq Check: 5307 from: 1 to: 5419
pFN21AE1730 5419 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2497 2740 2497
Size: 243 5176
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2582 3482 4866 5134 1632
Size: 749 201 900 1384 268 1917
Fragments arranged by size:
1917 1384 900 749 268 201
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 4318 5295 1203
Size: 3115 977 1327
Fragments arranged by size:
3115 1327 977
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5419
AlwNI CAG_nnn'CTG
Cuts at: 2656 3162 4734 2656
Size: 506 1572 3341
Fragments arranged by size:
3341 1572 506
ApaLI G'TGCA_C
Cuts at: 2635 3476 4632 2635
Size: 841 1156 3422
Fragments arranged by size:
3422 1156 841
ApoI r'AATT_y
Cuts at: 1493 2728 2954 3106 5215 1493
Size: 1235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2744 1870
Size: 73 801 4545
Fragments arranged by size:
4545 801 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2481 2514 2481
Size: 33 5386
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2186 2225 2497 2552 2566
Size: 325 217 627 399 39 272 55 14
Cuts at: 2566 2740 4195 618
Size: 174 1455 1842
Fragments arranged by size:
1842 1455 627 399 325 272 217 174
55 39 14
BanII G_rGCy'C
Cuts at: 1391 2738 1391
Size: 1347 4072
BbeI G_GCGC'C
Cuts at: 2190 2570 2190
Size: 380 5039
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 1903
Size: 34 1673 34 3678
Fragments arranged by size:
3678 1673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4030 136
Size: 107 122 71 631 605 2358 1525
Fragments arranged by size:
2358 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5414 5414
Size: 5419
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 2639 3480 4636 1790
Size: 849 841 1156 2573
Fragments arranged by size:
2573 1156 849 841
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 4108 442
Size: 416 53 83 399 24 1733 958 1753
Fragments arranged by size:
1753 1733 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 2312 2437 4079 1239
Size: 767 306 125 1642 2579
Fragments arranged by size:
2579 1642 767 306 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 4409 4707 3544
Size: 865 298 4256
Fragments arranged by size:
4256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3210 493
Size: 711 2006 2702
Fragments arranged by size:
2702 2006 711
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2451 2567 3226
Size: 53 83 186 936 654 264 116 659
Cuts at: 3226 3608 4243 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 659 654 635 382 264 186
116 83 53
BsaWI w'CCGG_w
Cuts at: 3852 4250 4524 4671 5082 3852
Size: 398 274 147 411 4189
Fragments arranged by size:
4189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4339
Fragments arranged by size:
4339 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5419
BseYI C'CCAG_C
Cuts at: 2068 2086 2349 4622 5118 2068
Size: 18 263 2273 496 2369
Fragments arranged by size:
2369 2273 496 263 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2669 1163
Size: 573 789 144 3913
Fragments arranged by size:
3913 789 573 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 3630 4658 1540
Size: 275 186 868 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2639 3480 3565 4636 2639
Size: 841 85 1071 3422
Fragments arranged by size:
3422 1071 841 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
BsmBI CGTCTCn'nnnn_
Cuts at: 2458 2573 2458
Size: 115 5304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2639 2738 3480 3565 4636 1391
Size: 399 849 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 849 742 399 99 85
BspEI T'CCGG_A
Cuts at: 5082 5082
Size: 5419
BspHI T'CATG_A
Cuts at: 3308 5038 3308
Size: 1730 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2178 2616 2774 844
Size: 1220 114 438 158 3489
Fragments arranged by size:
3489 1220 438 158 114
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 1394
Size: 429 1483 3507
Fragments arranged by size:
3507 1483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2183 4063 4250 1700
Size: 110 373 1880 187 2869
Fragments arranged by size:
2869 1880 373 187 110
BsrGI T'GTAC_A
Cuts at: 96 2481 96
Size: 2385 3034
BssHII G'CGCG_C
Cuts at: 1925 2009 2011 1925
Size: 84 2 5333
Fragments arranged by size:
5333 84 2
BssSI C'ACGA_G
Cuts at: 3479 4491 3479
Size: 1012 4407
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5419
BstBI TT'CG_AA
Cuts at: 4223 4223
Size: 5419
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5419
BstYI r'GATC_y
Cuts at: 1972 2652 2749 3116 3501 3518 4970 5056 5068
Size: 680 97 367 385 17 1452 86 12
Cuts at: 5068 5414 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 680 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2287 487
Size: 683 56 1061 3619
Fragments arranged by size:
3619 1061 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3729 3749 2935
Size: 794 20 4605
Fragments arranged by size:
4605 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DraIII CAC_nnn'GTG
Cuts at: 2559 2559
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2199 2866
Size: 54 1425 50 275 18 276 93 667
Cuts at: 2866 3755 5384 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 667 276 275 93 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2866 1537
Size: 275 1054 4090
Fragments arranged by size:
4090 1054 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2146 2318 3349 1353
Size: 713 80 172 1031 3423
Fragments arranged by size:
3423 1031 713 172 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4006 4380 4526 387
Size: 1064 138 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2312 2381 2437 2582 3482 4079
Size: 393 374 306 69 56 145 900 597
Cuts at: 4079 4866 5134 1239
Size: 787 268 1524
Fragments arranged by size:
1524 900 787 597 393 374 306 268
145 69 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 2291
Size: 5419
EcoRI G'AATT_C
Cuts at: 2728 3106 2728
Size: 378 5041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 5419
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 2190 2570 2645 4566 1678
Size: 512 380 75 1921 2531
Fragments arranged by size:
2531 1921 512 380 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373
Size: 32 135 32 2488 32 42 32 2626
Fragments arranged by size:
2626 2488 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5419
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KasI G'GCGC_C
Cuts at: 2186 2566 2186
Size: 380 5039
KpnI G_GTAC'C
Cuts at: 2501 2744 2501
Size: 243 5176
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 4533 4717 1305
Size: 1176 2052 184 2007
Fragments arranged by size:
2052 2007 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 10
Size: 54 1425 343 369 3228
Fragments arranged by size:
3228 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3377 3736 3895 518
Size: 648 1512 699 359 159 2042
Fragments arranged by size:
2042 1512 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2442 2835 3512 4660 4905 1606
Size: 836 393 677 1148 245 2120
Fragments arranged by size:
2120 1148 836 677 393 245
NarI GG'CG_CC
Cuts at: 2187 2567 2187
Size: 380 5039
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2777 4322 1132
Size: 97 1548 1545 2229
Fragments arranged by size:
2229 1548 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2415 1108
Size: 1307 4112
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2027 2059 2027
Size: 32 5387
PstI C_TGCA'G
Cuts at: 830 2771 830
Size: 1941 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2066
Size: 5419
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 3667 5379 3667
Size: 1712 3707
SexAI A'CCwGG_T
Cuts at: 2555 2555
Size: 5419
SfcI C'TryA_G
Cuts at: 826 1045 2767 3902 4583 4774 826
Size: 219 1722 1135 681 191 1471
Fragments arranged by size:
1722 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2188 2568 2188
Size: 380 5039
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 2183 1700
Size: 483 4936
SmaI CCC'GGG
Cuts at: 1872 2746 1872
Size: 874 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3523 4424 4686 820
Size: 197 926 1580 901 262 1553
Fragments arranged by size:
1580 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2777 1132
Size: 1645 3774
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5419
StyI C'CwwG_G
Cuts at: 513 1065 2004 2858 513
Size: 552 939 854 3074
Fragments arranged by size:
3074 939 854 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2213 2447 3446 3463 3616 3801
Size: 1255 498 135 234 999 17 153 185
Cuts at: 3801 4246 325
Size: 445 1498
Fragments arranged by size:
1498 1255 999 498 445 234 185 153
135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 2682 3665
Size: 274 80 33 51 1443 504 201 983
Cuts at: 3665 5377 96
Size: 1712 138
Fragments arranged by size:
1712 1443 983 504 274 201 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 5379 5411 2228
Size: 32 3119 32 2236
Fragments arranged by size:
3119 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2755 2755
Size: 5419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2209
Size: 5419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2744 1870
Size: 874 4545
XmnI GAAnn'nnTTC
Cuts at: 3548 3548
Size: 5419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SfiI SrfI SwaI