################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HgaI HincII HindII HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 157 GACGTC GACGT^C AciI 100 GCGG CCGC(-3/-1) AcyI 157 GACGTC G[GA]^CG[CT]C AfaI 193 GTAC GT^AC AvrII 33 CCTAGG C^CTAGG BbsI 154 GAAGAC GAAGAC(2/6) BfaI 34 CTAG C^TAG BglII 74 AGATCT A^GATCT BlnI 33 CCTAGG C^CTAGG BpuAI 154 GAAGAC GAAGAC(2/6) BsaHI 157 GACGTC G[GA]^CG[CT]C BsiEI 108 CGACCG CG[GA][CT]^CG BsmAI 217 GTCTC GTCTC(1/5) BsmBI 216 CGTCTC CGTCTC(1/5) BsrDI 162 CATTGC GCAATG(2/0) BsrFI 110 ACCGGC [GA]^CCGG[CT] BsrI 188 CCAGT ACTGG(1/-1) BstAPI 238 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 168 CCAGGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 74 AGATCT [GA]^GATC[CT] Cfr10I 110 ACCGGC [GA]^CCGG[CT] DpnII 75 GATC ^GATC EarI 307 GAAGAG CTCTTC(1/4) HaeII 55 AGCGCC [GA]GCGC^[CT] HaeIII 334 GGCC GG^CC HapII 111 CCGG C^CGG Hin1I 157 GACGTC G[GA]^CG[CT]C HindIII 181 AAGCTT A^AGCTT HpaII 111 CCGG C^CGG Ksp632I 307 GAAGAG CTCTTC(1/4) MaeI 34 CTAG C^TAG MboI 75 GATC ^GATC MflI 74 AGATCT [GA]^GATC[CT] MspI 111 CCGG C^CGG NdeII 75 GATC ^GATC NlaIII 324 CATG CATG^ RsaI 193 GTAC GT^AC Sau3AI 75 GATC ^GATC TaqI 95 TCGA T^CGA TthHB8I 95 TCGA T^CGA XhoII 74 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 134 AGCT AG^CT 182 AGCT AlwNI 230 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 239 CAGCCCCTG BsiYI 196 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 244 CCTGCTGCTGG BslI 196 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 244 CCTGCTGCTGG BspMI 20 ACCTGC ACCTGC(4/8) 195 ACCTGC BssKI 168 CCAGG ^CC[ACGT]GG 229 CCAGG BstNI 168 CCAGG CC^[AT]GG 229 CCAGG CfoI 3 GCGC GCG^C 56 GCGC EcoRII 168 CCAGG ^CC[AT]GG 229 CCAGG EcoT14I 33 CCTAGG C^C[AT][AT]GG 59 CCAAGG HhaI 3 GCGC GCG^C 56 GCGC HinP1I 3 GCGC G^CGC 56 GCGC HinfI 301 GAGTC G^A[ACGT]TC 310 GAGTC MaeII 8 ACGT A^CGT 158 ACGT MvaI 168 CCAGG CC^[AT]GG 229 CCAGG PleI 301 GAGTC GAGTC(4/5) 310 GAGTC PstI 236 CTGCAG CTGCA^G 266 CTGCAG ScrFI 168 CCAGG CC^[ACGT]GG 229 CCAGG StyI 33 CCTAGG C^C[AT][AT]GG 59 CCAAGG TspRI 129 CAGTG CA[GC]TG[ACGT][ACGT]^ 189 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BbvI BsaJI BseRI Cac8I Fnu4HI ItaI MboII MnlI MwoI SfcI TseI