Restriction map for
pFN21AE2256
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FspI HindIII HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 343 GACGTC GACGT^C
AfaI 508 GTAC GT^AC
Aor51HI 659 AGCGCT AGC^GCT
AspI 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 809 CCCGGG C^[CT]CG[GA]G
BanII 309 GAGCCC G[GA]GC[CT]^C
BbrPI 474 CACGTG CAC^GTG
BlpI 306 GCTGAGC GC^T[ACGT]AGC
BpmI 559 CTGGAG CTGGAG(16/14)
Bpu1102I 306 GCTGAGC GC^T[ACGT]AGC
BsgI 688 CTGCAC GTGCAG(16/14)
BsmFI 815 GTCCC GGGAC(10/14)
BsoBI 809 CCCGGG C^[CT]CG[GA]G
BsrI 635 ACTGG ACTGG(1/-1)
BssSI 160 CTCGTG CACGAG(-5/-1)
BstAPI 453 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 383 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 306 GCTGAGC GC^T[ACGT]AGC
DdeI 307 CTGAG C^T[ACGT]AG
DpnII 1008 GATC ^GATC
DrdI 571 GACTACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 268 CTCTTC CTCTTC(1/4)
Eco47III 659 AGCGCT AGC^GCT
EcoT14I 338 CCATGG C^C[AT][AT]GG
FseI 50 GGCCGGCC GGCCGG^CC
HincII 1171 GTCAAC GT[CT]^[GA]AC
HindII 1171 GTCAAC GT[CT]^[GA]AC
HinfI 972 GACTC G^A[ACGT]TC
Ksp632I 268 CTCTTC CTCTTC(1/4)
MaeIII 1153 GTCAC ^GT[ACGT]AC
MboI 1008 GATC ^GATC
NcoI 338 CCATGG C^CATGG
NdeII 1008 GATC ^GATC
PflFI 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 972 GACTC GAGTC(4/5)
PmaCI 474 CACGTG CAC^GTG
PmlI 474 CACGTG CAC^GTG
PvuII 454 CAGCTG CAG^CTG
RsaI 508 GTAC GT^AC
SapI 267 GCTCTTC GCTCTTC(1/4)
Sau3AI 1008 GATC ^GATC
SmaI 809 CCCGGG CCC^GGG
StyI 338 CCATGG C^C[AT][AT]GG
Tsp45I 1153 GTCAC ^GT[GC]AC
TspRI 1200 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 858 CCAGGACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 809 CCCGGG C^CCGGG
XmaI 809 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 274 GTCTAC GT^[AC][GT]AC
580 GTCTAC
AluI 455 AGCT AG^CT
686 AGCT
Alw44I 354 GTGCAC G^TGCAC
1093 GTGCAC
AlwNI 454 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1105 CAGGCGCTG
ApaLI 354 GTGCAC G^TGCAC
1093 GTGCAC
BalI 176 TGGCCA TGG^CCA
335 TGGCCA
BanI 469 GGCGCC G^G[CT][GA]CC
501 GGCGCC
BbeI 469 GGCGCC GGCGC^C
501 GGCGCC
BbsI 1204 GTCTTC GAAGAC(2/6)
1228 GTCTTC
BpuAI 1204 GTCTTC GAAGAC(2/6)
1228 GTCTTC
BsaAI 474 CACGTG [CT]AC^GT[GA]
574 TACGTG
BseRI 241 CTCCTC GAGGAG(10/8)
244 CTCCTC
BsrBI 265 CCGCTC CCGCTC(-3/-3)
616 CCGCTC
BssHII 186 GCGCGC G^CGCGC
698 GCGCGC
EcoNI 756 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
852 CCTCCCCCAGG
HphI 1010 TCACC GGTGA(8/7)
1154 TCACC
KasI 469 GGCGCC G^GCGCC
501 GGCGCC
MluNI 176 TGGCCA TGG^CCA
335 TGGCCA
MscI 176 TGGCCA TGG^CCA
335 TGGCCA
MslI 333 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
384 CATCAGCGTG
NaeI 51 GCCGGC GCC^GGC
449 GCCGGC
NarI 469 GGCGCC GG^CGCC
501 GGCGCC
NgoMIV 51 GCCGGC G^CCGGC
449 GCCGGC
NotI 229 GCGGCCGC GC^GGCCGC
533 GCGGCCGC
PstI 193 CTGCAG CTGCA^G
655 CTGCAG
SfaNI 64 GCATC GCATC(5/9)
76 GCATC
SfcI 193 CTGCAG C^T[GA][CT]AG
655 CTGCAG
SfoI 469 GGCGCC GGC^GCC
501 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AspHI AvaII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KspI MaeII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI SacII Sau96I ScrFI TseI