(Circular) (Six-base) MAPSORT of: pFN21AE2256.seq Check: 2136 from: 1 to: 5962 pFN21AE2256 5962 bp With 182 enzymes: SgfI * July 20, 2018 18:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2352 3772 278 Size: 53 83 186 936 816 1420 2468 Fragments arranged by size: 2468 1420 936 816 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 2586 3305 1937 Size: 343 306 719 4594 Fragments arranged by size: 4594 719 343 306 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AfeI AGC'GCT Cuts at: 2666 2666 Size: 5962 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3263 1184 Size: 2079 3883 AlwNI CAG_nnn'CTG Cuts at: 2464 3115 3705 5277 2464 Size: 651 590 1572 3149 Fragments arranged by size: 3149 1572 651 590 ApaLI G'TGCA_C Cuts at: 2359 3098 4019 5175 2359 Size: 739 921 1156 3146 Fragments arranged by size: 3146 1156 921 739 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2814 3287 1870 Size: 73 871 473 4545 Fragments arranged by size: 4545 871 473 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 2506 3283 4738 618 Size: 325 217 627 687 32 777 1455 1842 Fragments arranged by size: 1842 1455 777 687 627 325 217 32 BanII G_rGCy'C Cuts at: 1391 2318 3281 1391 Size: 927 963 4072 Fragments arranged by size: 4072 963 927 BbeI G_GCGC'C Cuts at: 2478 2510 2478 Size: 32 5930 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3202 3226 928 Size: 971 1303 24 3664 Fragments arranged by size: 3664 1303 971 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 2339 3142 Size: 107 122 71 631 605 394 273 803 Cuts at: 3142 4573 136 Size: 1431 1525 Fragments arranged by size: 1525 1431 803 631 605 394 273 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2312 3379 2312 Size: 1067 4895 Bme1580I G_kGCm'C Cuts at: 1790 2363 3102 3138 4023 5179 1790 Size: 573 739 36 885 1156 2573 Fragments arranged by size: 2573 1156 885 739 573 36 BmgBI CAC'GTC Cuts at: 1318 2533 2681 1318 Size: 1215 148 4599 Fragments arranged by size: 4599 1215 148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 3693 4651 Size: 416 53 83 399 24 1233 1043 958 Cuts at: 4651 442 Size: 1753 Fragments arranged by size: 1753 1233 1043 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 4622 1239 Size: 1346 2037 2579 Fragments arranged by size: 2579 2037 1346 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2481 2581 3753 493 Size: 711 1277 100 1172 2702 Fragments arranged by size: 2702 1277 1172 711 100 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 2331 2349 2475 Size: 53 83 186 936 671 127 18 126 Cuts at: 2475 2507 2946 3083 3769 4151 4786 275 Size: 32 439 137 686 382 635 1451 Fragments arranged by size: 1451 936 686 671 635 439 382 186 137 127 126 83 53 32 18 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2989 3019 1178 Size: 30 165 30 1586 30 4121 Fragments arranged by size: 4121 1586 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2240 1186 Size: 1051 3 4908 Fragments arranged by size: 4908 1051 3 BseYI C'CCAG_C Cuts at: 2045 2096 2667 2717 2852 3166 5165 5661 2045 Size: 51 571 50 135 314 1999 496 2346 Fragments arranged by size: 2346 1999 571 496 314 135 51 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 1163 Size: 573 942 4447 Fragments arranged by size: 4447 942 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2238 2542 2701 3022 3412 Size: 275 186 60 177 304 159 321 390 Cuts at: 3412 4173 5201 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 390 321 304 275 186 177 159 60 BsiHKAI G_wGCw'C Cuts at: 2151 2363 2603 2938 3102 4023 4108 5179 2151 Size: 212 240 335 164 921 85 1071 2934 Fragments arranged by size: 2934 1071 921 335 240 212 164 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2318 2363 2603 2938 3102 3138 Size: 399 361 167 45 240 335 164 36 Cuts at: 3138 3281 4023 4108 5179 1391 Size: 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 335 240 167 164 143 85 45 36 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3317 844 Size: 2473 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2272 2623 3849 1394 Size: 429 449 351 1226 3507 Fragments arranged by size: 3507 1226 449 429 351 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2436 2454 3017 3049 4606 4793 Size: 110 246 380 18 563 32 1557 187 Cuts at: 4793 1700 Size: 2869 Fragments arranged by size: 2869 1557 563 380 246 187 110 32 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 2191 2703 1925 Size: 266 512 5184 Fragments arranged by size: 5184 512 266 BssSI C'ACGA_G Cuts at: 2165 4022 5034 2165 Size: 1857 1012 3093 Fragments arranged by size: 3093 1857 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2464 794 Size: 1670 4292 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2395 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957 Size: 1320 367 385 17 1452 86 12 346 Cuts at: 5957 1972 Size: 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 2061 2343 2412 2493 2653 Size: 552 939 18 39 282 69 81 160 Cuts at: 2653 3140 513 Size: 487 3335 Fragments arranged by size: 3335 939 552 487 282 160 81 69 39 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 4969 809 Size: 507 1266 2387 1802 Fragments arranged by size: 2387 1802 1266 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2181 2235 Size: 54 1425 50 275 18 228 123 54 Cuts at: 2235 2340 2409 2415 2539 2698 3019 3409 4298 Size: 105 69 6 124 159 321 390 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 390 321 275 228 159 124 123 105 69 54 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2058 2235 2539 2698 3019 3409 1537 Size: 275 246 177 304 159 321 390 4090 Fragments arranged by size: 4090 390 321 304 275 246 177 159 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 3892 1353 Size: 926 1613 3423 Fragments arranged by size: 3423 1613 926 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 4025 4622 5409 5677 1239 Size: 393 953 1440 597 787 268 1524 Fragments arranged by size: 1524 1440 953 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2861 2765 Size: 96 5866 EcoO109I rG'GnC_Cy Cuts at: 2770 2819 2866 3043 3063 3244 2770 Size: 49 47 177 20 181 5488 Fragments arranged by size: 5488 181 177 49 47 20 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FseI GG_CCGG'CC Cuts at: 2060 2060 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2117 2478 2510 2668 3116 3156 3186 5109 Size: 439 361 32 158 448 40 30 1923 Cuts at: 5109 1678 Size: 2531 Fragments arranged by size: 2531 1923 448 439 361 158 40 32 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4603 4635 4677 4709 1373 Size: 32 135 32 3031 32 42 32 2626 Fragments arranged by size: 3031 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3306 3552 669 Size: 988 281 1240 128 246 3079 Fragments arranged by size: 3079 1240 988 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KasI G'GCGC_C Cuts at: 2474 2506 2474 Size: 32 5930 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2952 5076 5260 1305 Size: 1647 2124 184 2007 Fragments arranged by size: 2124 2007 1647 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 2342 10 Size: 54 1425 343 351 159 3630 Fragments arranged by size: 3630 1425 351 343 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2393 3920 4279 4438 518 Size: 648 1176 51 1527 359 159 2042 Fragments arranged by size: 2042 1527 1176 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2024 2063 2425 2461 2495 2655 2721 3142 Size: 418 39 362 36 34 160 66 421 Cuts at: 3142 3378 4055 5203 5448 1606 Size: 236 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 421 418 362 245 236 160 66 39 36 34 NaeI GCC'GGC Cuts at: 2058 2456 2058 Size: 398 5564 NarI GG'CG_CC Cuts at: 2475 2507 2475 Size: 32 5930 NcoI C'CATG_G Cuts at: 513 1065 2004 2343 513 Size: 552 939 339 4132 Fragments arranged by size: 4132 939 552 339 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 2056 2454 2056 Size: 398 5564 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 2235 2539 3409 2235 Size: 304 870 4788 Fragments arranged by size: 4788 870 304 NspI r_CATG'y Cuts at: 1132 1229 3320 4865 1132 Size: 97 2091 1545 2229 Fragments arranged by size: 2229 2091 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PmlI CAC'GTG Cuts at: 2481 2481 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2770 2819 2866 3043 2770 Size: 49 47 177 5689 Fragments arranged by size: 5689 177 49 47 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2202 2664 3314 830 Size: 1372 462 650 3478 Fragments arranged by size: 3478 1372 650 462 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5962 SacII CC_GC'GG Cuts at: 2025 2064 2496 2656 3143 2025 Size: 39 432 160 487 4844 Fragments arranged by size: 4844 487 432 160 39 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SanDI GG'GwC_CC Cuts at: 2819 2819 Size: 5962 SapI GCTCTTCn'nnn_ Cuts at: 2279 2279 Size: 5962 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2198 2660 3310 4445 5126 5317 826 Size: 219 1153 462 650 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 681 650 462 219 191 SfoI GGC'GCC Cuts at: 2476 2508 2476 Size: 32 5930 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2816 3289 1872 Size: 944 473 4545 Fragments arranged by size: 4545 944 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2343 3401 513 Size: 552 939 339 1058 3074 Fragments arranged by size: 3074 1058 939 552 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2989 3021 5922 5954 2989 Size: 32 2901 32 2997 Fragments arranged by size: 2997 2901 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2948 1316 Size: 1632 4330 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2870 2870 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2814 3287 1870 Size: 944 473 4545 Fragments arranged by size: 4545 944 473 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2350 3770 276 Size: 53 83 186 936 816 1420 2468 Fragments arranged by size: 2468 1420 936 816 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI