Restriction map for
pFN21AE2256
(Linear) (Six-base) MAPSORT of: KIEE2256.seq Check: 1637 from: 1 to: 1257
KIEE2256.orf
With 182 enzymes: SgfI *
July 20, 2018 17:51 ..
AatII G_ACGT'C
Cuts at: 0 347 1257
Size: 347 910
AccI GT'mk_AC
Cuts at: 0 275 581 1257
Size: 275 306 676
Fragments arranged by size:
676 306 275
AfeI AGC'GCT
Cuts at: 0 661 1257
Size: 661 596
AlwNI CAG_nnn'CTG
Cuts at: 0 459 1110 1257
Size: 459 651 147
Fragments arranged by size:
651 459 147
ApaLI G'TGCA_C
Cuts at: 0 354 1093 1257
Size: 354 739 164
Fragments arranged by size:
739 354 164
AvaI C'yCGr_G
Cuts at: 0 809 1257
Size: 809 448
BanI G'GyrC_C
Cuts at: 0 469 501 1257
Size: 469 32 756
Fragments arranged by size:
756 469 32
BanII G_rGCy'C
Cuts at: 0 313 1257
Size: 313 944
BbeI G_GCGC'C
Cuts at: 0 473 505 1257
Size: 473 32 752
Fragments arranged by size:
752 473 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1197 1221 1257
Size: 1197 24 36
Fragments arranged by size:
1197 36 24
BglI GCCn_nnn'nGGC
Cuts at: 0 61 334 1137 1257
Size: 61 273 803 120
Fragments arranged by size:
803 273 120 61
BlpI GC'TnA_GC
Cuts at: 0 307 1257
Size: 307 950
Bme1580I G_kGCm'C
Cuts at: 0 358 1097 1133 1257
Size: 358 739 36 124
Fragments arranged by size:
739 358 124 36
BmgBI CAC'GTC
Cuts at: 0 528 676 1257
Size: 528 148 581
Fragments arranged by size:
581 528 148
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 1257
Size: 645 612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1257
Size: 580 677
BsaAI yAC'GTr
Cuts at: 0 476 576 1257
Size: 476 100 681
Fragments arranged by size:
681 476 100
BsaHI Gr'CG_yC
Cuts at: 0 199 326 344 470 502 941 1078 1257
Size: 199 127 18 126 32 439 137 179
Fragments arranged by size:
439 199 179 137 127 126 32 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 984 1014 1257
Size: 984 30 243
Fragments arranged by size:
984 243 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 235 1257
Size: 232 3 1022
Fragments arranged by size:
1022 232 3
BseYI C'CCAG_C
Cuts at: 0 40 91 662 712 847 1161 1257
Size: 40 51 571 50 135 314 96
Fragments arranged by size:
571 314 135 96 51 50 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1257
Size: 673 584
BsiEI CG_ry'CG
Cuts at: 0 56 233 537 696 1017 1257
Size: 56 177 304 159 321 240
Fragments arranged by size:
321 304 240 177 159 56
BsiHKAI G_wGCw'C
Cuts at: 0 146 358 598 933 1097 1257
Size: 146 212 240 335 164 160
Fragments arranged by size:
335 240 212 164 160 146
Bsp1286I G_dGCh'C
Cuts at: 0 146 313 358 598 933 1097 1133 1257
Size: 146 167 45 240 335 164 36 124
Fragments arranged by size:
335 240 167 164 146 124 45 36
BsrBI CCG'CTC
Cuts at: 0 267 618 1257
Size: 267 351 639
Fragments arranged by size:
639 351 267
BsrFI r'CCGG_y
Cuts at: 0 51 431 449 1012 1044 1257
Size: 51 380 18 563 32 213
Fragments arranged by size:
563 380 213 51 32 18
BssHII G'CGCG_C
Cuts at: 0 186 698 1257
Size: 186 512 559
Fragments arranged by size:
559 512 186
BssSI C'ACGA_G
Cuts at: 0 160 1257
Size: 160 1097
BstAPI GCAn_nnn'nTGC
Cuts at: 0 459 1257
Size: 459 798
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 1257
Size: 390 867
BtgI C'CryG_G
Cuts at: 0 17 56 338 407 488 648 1135 1257
Size: 17 39 282 69 81 160 487 122
Fragments arranged by size:
487 282 160 122 81 69 39 17
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 1257
Size: 577 680
EaeI y'GGCC_r
Cuts at: 0 53 176 230 335 404 410 534 693
Size: 53 123 54 105 69 6 124 159
Cuts at: 693 1014 1257
Size: 321 243
Fragments arranged by size:
321 243 159 124 123 105 69 54
53 6
EagI C'GGCC_G
Cuts at: 0 53 230 534 693 1014 1257
Size: 53 177 304 159 321 243
Fragments arranged by size:
321 304 243 177 159 53
EarI CTCTTCn'nnn_
Cuts at: 0 274 1257
Size: 274 983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1257
Size: 580 677
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 856 1257
Size: 760 96 401
Fragments arranged by size:
760 401 96
EcoO109I rG'GnC_Cy
Cuts at: 0 765 814 861 1038 1058 1239 1257
Size: 765 49 47 177 20 181 18
Fragments arranged by size:
765 181 177 49 47 20 18
FseI GG_CCGG'CC
Cuts at: 0 55 1257
Size: 55 1202
HaeII r_GCGC'y
Cuts at: 0 112 473 505 663 1111 1151 1181 1257
Size: 112 361 32 158 448 40 30 76
Fragments arranged by size:
448 361 158 112 76 40 32 30
HincII GTy'rAC
Cuts at: 0 1173 1257
Size: 1173 84
KasI G'GCGC_C
Cuts at: 0 469 501 1257
Size: 469 32 756
Fragments arranged by size:
756 469 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1257
Size: 947 310
MscI TGG'CCA
Cuts at: 0 178 337 1257
Size: 178 159 920
Fragments arranged by size:
920 178 159
MslI CAynn'nnrTG
Cuts at: 0 337 388 1257
Size: 337 51 869
Fragments arranged by size:
869 337 51
MspA1I CmG'CkG
Cuts at: 0 19 58 420 456 490 650 716 1137
Size: 19 39 362 36 34 160 66 421
Cuts at: 1137 1257
Size: 120
Fragments arranged by size:
421 362 160 120 66 39 36 34
19
NaeI GCC'GGC
Cuts at: 0 53 451 1257
Size: 53 398 806
Fragments arranged by size:
806 398 53
NarI GG'CG_CC
Cuts at: 0 470 502 1257
Size: 470 32 755
Fragments arranged by size:
755 470 32
NcoI C'CATG_G
Cuts at: 0 338 1257
Size: 338 919
NgoMIV G'CCGG_C
Cuts at: 0 51 449 1257
Size: 51 398 808
Fragments arranged by size:
808 398 51
NotI GC'GGCC_GC
Cuts at: 0 230 534 1257
Size: 230 304 723
Fragments arranged by size:
723 304 230
PmlI CAC'GTG
Cuts at: 0 476 1257
Size: 476 781
PpuMI rG'GwC_Cy
Cuts at: 0 765 814 861 1038 1257
Size: 765 49 47 177 219
Fragments arranged by size:
765 219 177 49 47
PstI C_TGCA'G
Cuts at: 0 197 659 1257
Size: 197 462 598
Fragments arranged by size:
598 462 197
PvuII CAG'CTG
Cuts at: 0 456 1257
Size: 456 801
SacII CC_GC'GG
Cuts at: 0 20 59 491 651 1138 1257
Size: 20 39 432 160 487 119
Fragments arranged by size:
487 432 160 119 39 20
SanDI GG'GwC_CC
Cuts at: 0 814 1257
Size: 814 443
SapI GCTCTTCn'nnn_
Cuts at: 0 274 1257
Size: 274 983
SfcI C'TryA_G
Cuts at: 0 193 655 1257
Size: 193 462 602
Fragments arranged by size:
602 462 193
SfoI GGC'GCC
Cuts at: 0 471 503 1257
Size: 471 32 754
Fragments arranged by size:
754 471 32
SmaI CCC'GGG
Cuts at: 0 811 1257
Size: 811 446
StyI C'CwwG_G
Cuts at: 0 338 1257
Size: 338 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1257
Size: 984 32 241
Fragments arranged by size:
984 241 32
Tth111I GACn'n_nGTC
Cuts at: 0 943 1257
Size: 943 314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 865 1257
Size: 865 392
XmaI C'CCGG_G
Cuts at: 0 809 1257
Size: 809 448
ZraI GAC'GTC
Cuts at: 0 345 1257
Size: 345 912
Enzymes that do cut:
AatII AccI AfeI AlwNI ApaLI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstXI BtgI
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI
HaeII HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI PmlI PpuMI PstI PvuII
SacII SanDI SapI SfcI SfoI SmaI StyI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FalI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI XbaI XhoI XmnI