################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmAI BsmBI BspLU11I BsrBI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MslI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI SspI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 236 TCCGGA T^CCGGA AlwNI 402 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1005 GAGCTC G[AT]GC[AT]^C BalI 584 TGGCCA TGG^CCA BanI 222 GGTGCC G^G[CT][GA]CC BcgI 132 CGATGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1117 CTAG C^TAG BglII 936 AGATCT A^GATCT BlpI 385 GCTGAGC GC^T[ACGT]AGC Bpu1102I 385 GCTGAGC GC^T[ACGT]AGC BsaWI 236 TCCGGA [AT]^CCGG[AT] BseAI 236 TCCGGA T^CCGGA BsiHKAI 1005 GAGCTC G[AT]GC[AT]^C Bsp1407I 491 TGTACA T^GTACA BspDI 688 ATCGAT AT^CGAT BspEI 236 TCCGGA T^CCGGA BspHI 648 TCATGA T^CATGA BspMI 763 ACCTGC ACCTGC(4/8) BsrGI 491 TGTACA T^GTACA BstAPI 767 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 936 AGATCT [GA]^GATC[CT] CelII 385 GCTGAGC GC^T[ACGT]AGC ClaI 688 ATCGAT AT^CGAT EaeI 584 TGGCCA [CT]^GGCC[GA] EarI 1141 GAAGAG CTCTTC(1/4) HinfI 393 GATTC G^A[ACGT]TC HphI 172 GGTGA GGTGA(8/7) Ksp632I 1141 GAAGAG CTCTTC(1/4) MaeI 1117 CTAG C^TAG MflI 936 AGATCT [GA]^GATC[CT] MluNI 584 TGGCCA TGG^CCA MroI 236 TCCGGA T^CCGGA MscI 584 TGGCCA TGG^CCA MseI 284 TTAA T^TAA NaeI 436 GCCGGC GCC^GGC NgoMIV 436 GCCGGC G^CCGGC NspI 912 GCATGT [GA]CATG^[CT] PflMI 411 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 373 CTGCAG CTGCA^G PvuII 1084 CAGCTG CAG^CTG RcaI 648 TCATGA T^CATGA SacI 1005 GAGCTC GAGCT^C SbfI 372 CCTGCAGG CCTGCA^GG SfcI 373 CTGCAG C^T[GA][CT]AG SgrAI 849 CACCGGCG C[GA]^CCGG[CT]G SmlI 697 CTTGAG C^T[CT][GA]AG Sse8387I 372 CCTGCAGG CCTGCA^GG SspBI 491 TGTACA T^GTACA TfiI 393 GATTC G^A[AT]TC Tru9I 284 TTAA T^TAA Van91I 411 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 936 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 164 GGGCCC GGGCC^C 1045 GGGCCC AscI 17 GGCGCGCC GG^CGCGCC 189 GGCGCGCC AvaI 217 CCCGAG C^[CT]CG[GA]G 526 CCCGAG BpmI 502 CTCCAG CTGGAG(16/14) 964 CTGGAG BsmI 232 GCATTC GAATGC(1/-1) 458 GCATTC BsoBI 217 CCCGAG C^[CT]CG[GA]G 526 CCCGAG BsrFI 436 GCCGGC [GA]^CCGG[CT] 850 ACCGGC BssHII 18 GCGCGC G^CGCGC 190 GCGCGC Cfr10I 436 GCCGGC [GA]^CCGG[CT] 850 ACCGGC DraIII 449 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1102 CACGGCGTG EcoNI 197 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 407 CCTGCCAGAGG HindIII 364 AAGCTT A^AGCTT 1177 AAGCTT KspI 23 CCGCGG CCGC^GG 168 CCGCGG MaeIII 353 GTGAC ^GT[ACGT]AC 622 GTCAC NcoI 159 CCATGG C^CATGG 929 CCATGG PpuMI 955 GGGACCT [GA]G^G[AT]CC[CT] 1151 GGGACCT SacII 23 CCGCGG CCGC^GG 168 CCGCGG StuI 95 AGGCCT AGG^CCT 205 AGGCCT Tsp45I 353 GTGAC ^GT[GC]AC 622 GTCAC TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^ 453 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TthHB8I