(Circular) (Six-base) MAPSORT of: pFN21AE9618.seq Check: 5254 from: 1 to: 5902 pFN21AE9618 5902 bp With 182 enzymes: SgfI * March 28, 2012 12:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2777 2777 Size: 5902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2458 3965 5349 5617 1632 Size: 826 1507 1384 268 1917 Fragments arranged by size: 1917 1507 1384 826 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 2412 3645 5217 2412 Size: 1233 1572 3097 Fragments arranged by size: 3097 1572 1233 ApaI G_GGCC'C Cuts at: 2173 3054 2173 Size: 881 5021 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 3211 3437 3589 5698 1493 Size: 1718 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 226 152 AscI GG'CGCG_CC Cuts at: 2023 2195 2023 Size: 172 5730 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2222 2531 3227 1870 Size: 73 279 309 696 4545 Fragments arranged by size: 4545 696 309 279 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 3223 4678 618 Size: 325 217 627 440 996 1455 1842 Fragments arranged by size: 1842 1455 996 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2173 2184 3014 3054 3221 1391 Size: 782 11 830 40 167 4072 Fragments arranged by size: 4072 830 782 167 40 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2126 2160 4093 4127 1903 Size: 34 189 34 1933 34 3678 Fragments arranged by size: 3678 1933 189 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2941 5897 2941 Size: 2956 2946 BlpI GC'TnA_GC Cuts at: 2391 3319 2391 Size: 928 4974 Bme1580I G_kGCm'C Cuts at: 1790 2173 2232 3054 3963 5119 1790 Size: 383 59 822 909 1156 2573 Fragments arranged by size: 2573 1156 909 822 383 59 BmgBI CAC'GTC Cuts at: 1318 2818 3082 3100 1318 Size: 1500 264 18 4120 Fragments arranged by size: 4120 1500 264 18 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2262 2451 3186 Size: 416 53 83 399 24 845 189 735 Cuts at: 3186 3633 4591 442 Size: 447 958 1753 Fragments arranged by size: 1753 958 845 735 447 416 399 189 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2990 4562 1239 Size: 1253 498 1572 2579 Fragments arranged by size: 2579 1572 1253 498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4027 4892 5190 2723 Size: 1304 865 298 3435 Fragments arranged by size: 3435 1304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2241 4335 4733 5007 5154 5565 2241 Size: 2094 398 274 147 411 2578 Fragments arranged by size: 2578 2094 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5902 BseYI C'CCAG_C Cuts at: 3087 3091 5105 5601 3087 Size: 4 2014 496 3388 Fragments arranged by size: 3388 2014 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2755 2836 3185 1163 Size: 573 1019 81 349 3880 Fragments arranged by size: 3880 1019 573 349 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3014 3963 4048 5119 3014 Size: 949 85 1071 3797 Fragments arranged by size: 3797 1071 949 85 BsmI GAATG_Cn' Cuts at: 2237 2463 3413 3506 2237 Size: 226 950 93 4633 Fragments arranged by size: 4633 950 226 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 2184 2232 3014 3054 3221 3963 Size: 399 383 11 48 782 40 167 742 Cuts at: 3963 4048 5119 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 399 383 167 85 48 40 11 BspEI T'CCGG_A Cuts at: 2241 5565 2241 Size: 3324 2578 BspHI T'CATG_A Cuts at: 2653 3791 5521 2653 Size: 1138 1730 3034 Fragments arranged by size: 3034 1730 1138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 3257 844 Size: 1933 480 3489 Fragments arranged by size: 3489 1933 480 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2441 2855 4546 4733 1700 Size: 110 631 414 1691 187 2869 Fragments arranged by size: 2869 1691 631 414 187 110 BsrGI T'GTAC_A Cuts at: 96 2496 96 Size: 2400 3502 BssHII G'CGCG_C Cuts at: 1925 2023 2195 1925 Size: 98 172 5632 Fragments arranged by size: 5632 172 98 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 2778 794 Size: 1984 3918 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2941 3232 3599 3984 4001 5453 5539 5551 Size: 969 291 367 385 17 1452 86 12 Cuts at: 5551 5897 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 969 385 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2164 2173 2934 513 Size: 552 939 24 136 9 761 3481 Fragments arranged by size: 3481 939 761 552 136 24 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2362 3418 4212 4232 2362 Size: 1056 794 20 4032 Fragments arranged by size: 4032 1056 794 20 ClaI AT'CG_AT Cuts at: 1554 2694 1554 Size: 1140 4762 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DraIII CAC_nnn'GTG Cuts at: 2459 3112 2459 Size: 653 5249 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 3349 4238 Size: 54 1425 50 275 18 759 760 889 Cuts at: 4238 5867 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 760 759 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3141 3832 1353 Size: 1788 691 3423 Fragments arranged by size: 3423 1788 691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3012 3012 Size: 5902 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2458 2492 2990 3965 4562 5349 5617 Size: 393 826 34 498 975 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 975 826 787 597 498 393 268 34 EcoNI CCTnn'n_nnAGG Cuts at: 2206 2416 2206 Size: 210 5692 EcoO109I rG'GnC_Cy Cuts at: 2169 2961 3050 3051 3157 2169 Size: 792 89 1 106 4914 Fragments arranged by size: 4914 792 106 89 1 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 5049 1678 Size: 3371 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2696 2728 3015 Size: 32 135 32 890 32 202 32 287 Cuts at: 3015 3047 4543 4575 4617 4649 1373 Size: 32 1496 32 42 32 2626 Fragments arranged by size: 2626 1496 890 287 202 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 2369 3182 748 Size: 1621 813 3468 Fragments arranged by size: 3468 1621 813 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2320 5016 5200 1305 Size: 1015 2696 184 2007 Fragments arranged by size: 2696 2007 1015 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3321 Fragments arranged by size: 3321 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2175 2841 2896 3091 3169 3318 3995 Size: 424 145 666 55 195 78 149 677 Cuts at: 3995 5143 5388 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 424 245 195 149 145 78 55 NaeI GCC'GGC Cuts at: 2443 2443 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 2004 2164 2934 513 Size: 552 939 160 770 3481 Fragments arranged by size: 3481 939 770 552 160 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NgoMIV G'CCGG_C Cuts at: 2441 2441 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2921 3260 4805 1132 Size: 97 1692 339 1545 2229 Fragments arranged by size: 2229 1692 1545 339 97 PasI CC'CwG_GG Cuts at: 1348 2232 2795 1348 Size: 884 563 4455 Fragments arranged by size: 4455 884 563 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2422 1108 Size: 1314 4588 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2961 3157 2961 Size: 196 5706 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PspOMI G'GGCC_C Cuts at: 2169 3050 2169 Size: 881 5021 PstI C_TGCA'G Cuts at: 830 2382 3254 830 Size: 1552 872 3478 Fragments arranged by size: 3478 1552 872 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 3091 3091 Size: 5902 SacI G_AGCT'C Cuts at: 3014 3014 Size: 5902 SacII CC_GC'GG Cuts at: 2031 2176 2031 Size: 145 5757 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 2382 3254 2382 Size: 872 5030 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2378 3250 4385 5066 5257 826 Size: 219 1333 872 1135 681 191 1471 Fragments arranged by size: 1471 1333 1135 872 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 2855 1700 Size: 1155 4747 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 4006 4907 5169 820 Size: 197 926 759 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 759 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 2102 2212 1517 Size: 585 110 5207 Fragments arranged by size: 5207 585 110 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 2184 2592 2934 2956 3054 Size: 552 939 160 20 408 342 22 98 Cuts at: 3054 3341 513 Size: 287 3074 Fragments arranged by size: 3074 939 552 408 342 287 160 98 22 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 2470 3011 3929 3946 4099 4284 Size: 1255 732 158 541 918 17 153 185 Cuts at: 4284 4729 325 Size: 445 1498 Fragments arranged by size: 1498 1255 918 732 541 445 185 158 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4148 5860 Size: 274 80 33 51 1443 519 1652 1712 Cuts at: 5860 96 Size: 138 Fragments arranged by size: 1712 1652 1443 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI