Restriction map for
pFN21AE2339
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoRI EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MluI MroI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 102 GTAC GT^AC
AgeI 580 ACCGGT A^CCGGT
Asp700I 219 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 202 GAGCTC G[AT]GC[AT]^C
AvaI 84 CTCGGG C^[CT]CG[GA]G
BalI 716 TGGCCA TGG^CCA
BanI 523 GGCACC G^G[CT][GA]CC
BanII 202 GAGCTC G[GA]GC[CT]^C
BbsI 219 GAAGAC GAAGAC(2/6)
BbvCI 42 GCTGAGG CCTCAGC(-5/-2)
BglI 241 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 491 AGATCT A^GATCT
BmyI 202 GAGCTC G[AGT]GC[ACT]^C
BpuAI 219 GAAGAC GAAGAC(2/6)
BseRI 229 GAGGAG GAGGAG(10/8)
BsgI 674 GTGCAG GTGCAG(16/14)
BsiEI 239 CGGCCG CG[GA][CT]^CG
BsiHKAI 202 GAGCTC G[AT]GC[AT]^C
BsmI 732 GAATGC GAATGC(1/-1)
BsoBI 84 CTCGGG C^[CT]CG[GA]G
Bsp1286I 202 GAGCTC G[AGT]GC[ACT]^C
BspMI 616 GCAGGT ACCTGC(4/8)
BstEII 583 GGTGACC G^GT[ACGT]ACC
BstPI 583 GGTGACC G^GT[ACGT]ACC
EagI 239 CGGCCG C^GGCCG
EclXI 239 CGGCCG C^GGCCG
Eco52I 239 CGGCCG C^GGCCG
EcoNI 366 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 583 GGTGACC G^GT[ACGT]ACC
EcoT14I 164 CCAAGG C^C[AT][AT]GG
KspI 33 CCGCGG CCGC^GG
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
NlaIII 438 CATG CATG^
PinAI 580 ACCGGT A^CCGGT
PleI 533 GACTC GAGTC(4/5)
PpuMI 233 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 284 CAGCTG CAG^CTG
RsaI 102 GTAC GT^AC
SacI 202 GAGCTC GAGCT^C
SacII 33 CCGCGG CCGC^GG
SfiI 240 GGCCGTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 275 AGGCCT AGG^CCT
StyI 164 CCAAGG C^C[AT][AT]GG
TaqI 227 TCGA T^CGA
TfiI 724 GAATC G^A[AT]TC
Tsp45I 584 GTGAC ^GT[GC]AC
TthHB8I 227 TCGA T^CGA
XmnI 219 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 34 CGCG CG^CG
831 CGCG
AcyI 71 GGCGTC G[GA]^CG[CT]C
604 GACGCC
AlwI 147 GATCC GGATC(4/5)
590 GGATC
AlwNI 211 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
310 CAGAACCTG
BcnI 51 CCCGG CC^[GC]GG
237 CCCGG
BpmI 336 CTCCAG CTGGAG(16/14)
287 CTGGAG
BsaHI 71 GGCGTC G[GA]^CG[CT]C
604 GACGCC
BsmAI 531 GAGAC GTCTC(1/5)
646 GAGAC
BstUI 34 CGCG CG^CG
831 CGCG
BstYI 491 AGATCT [GA]^GATC[CT]
590 GGATCT
EcoRV 295 GATATC GAT^ATC
448 GATATC
Hin1I 71 GGCGTC G[GA]^CG[CT]C
604 GACGCC
HinfI 533 GACTC G^A[ACGT]TC
724 GAATC
MaeIII 332 GTTAC ^GT[ACGT]AC
584 GTGAC
MflI 491 AGATCT [GA]^GATC[CT]
590 GGATCT
MseI 496 TTAA T^TAA
754 TTAA
MvnI 34 CGCG CG^CG
831 CGCG
NciI 51 CCCGG CC^[GC]GG
237 CCCGG
SgrAI 261 CACCGGCG C[GA]^CCGG[CT]G
579 CACCGGTG
Tru9I 496 TTAA T^TAA
754 TTAA
XhoII 491 AGATCT [GA]^GATC[CT]
590 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsaWI BsiYI BslI BsmFI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp509I TspRI