Restriction map for
pFN21AE2339
(Circular) (Six-base) MAPSORT of: pFN21AE2339.seq Check: 389 from: 1 to: 5575
pFN21AE2339 5575 bp
With 182 enzymes: SgfI *
May 9, 2016 12:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 3638 5022 5290 1632
Size: 611 1395 1384 268 1917
Fragments arranged by size:
1917 1395 1384 611 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 2585 4406 2585
Size: 1821 3754
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2221 2320 3318 4890 2221
Size: 99 998 1572 2906
Fragments arranged by size:
2906 1572 998 99
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2900 1870
Size: 73 146 811 4545
Fragments arranged by size:
4545 811 146 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 2896 4351 618
Size: 325 217 627 741 368 1455 1842
Fragments arranged by size:
1842 1455 741 627 368 325 217
BanII G_rGCy'C
Cuts at: 1391 2211 2894 1391
Size: 820 683 4072
Fragments arranged by size:
4072 820 683
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 928
Size: 971 332 4272
Fragments arranged by size:
4272 971 332
BbvCI CC'TCA_GC
Cuts at: 2048 2048
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 4186 136
Size: 107 122 71 631 605 580 1934 1525
Fragments arranged by size:
1934 1525 631 605 580 122 107 71
BglII A'GATC_T
Cuts at: 2496 5570 2496
Size: 3074 2501
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 2473 3306
Size: 416 53 83 399 24 954 102 833
Cuts at: 3306 4264 442
Size: 958 1753
Fragments arranged by size:
1753 958 954 833 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2313 2326 4235 1239
Size: 1074 13 1909 2579
Fragments arranged by size:
2579 1909 1074 13
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2077 2610 3382 3764
Size: 53 83 186 936 544 533 772 382
Cuts at: 3764 4399 275
Size: 635 1451
Fragments arranged by size:
1451 936 772 635 544 533 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2585 2592 2817 4008 4406 4680 4827 5238 2585
Size: 7 225 1191 398 274 147 411 2922
Fragments arranged by size:
2922 1191 411 398 274 225 147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 1178
Size: 30 165 30 921 30 4399
Fragments arranged by size:
4399 921 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 4512
BseYI C'CCAG_C
Cuts at: 4778 5274 4778
Size: 496 5079
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2700 1163
Size: 573 964 4038
Fragments arranged by size:
4038 964 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2247 3025 3786 4814 1540
Size: 275 186 246 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2211 3636 3721 4792 2211
Size: 1425 85 1071 2994
Fragments arranged by size:
2994 1425 1071 85
BsmI GAATG_Cn'
Cuts at: 2743 3086 3179 2743
Size: 343 93 5139
Fragments arranged by size:
5139 343 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2894 3636 3721 4792 1391
Size: 399 421 683 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 421 399 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2612 2930 844
Size: 1768 318 3489
Fragments arranged by size:
3489 1768 318
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2465 2585 4219 4406 1700
Size: 110 457 198 120 1634 187 2869
Fragments arranged by size:
2869 1634 457 198 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 2588 1184
Size: 1404 4171
BstYI r'GATC_y
Cuts at: 1972 2496 2595 2905 3272 3657 3674 5126 5212
Size: 524 99 310 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 524 385 367 346 310 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2142 2247 513
Size: 552 939 34 104 105 3841
Fragments arranged by size:
3841 939 552 105 104 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2098 2205 2380 2582 2732 487
Size: 683 56 872 107 175 202 150 3330
Fragments arranged by size:
3330 872 683 202 175 150 107 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2244 2721
Size: 54 1425 50 275 18 177 237 477
Cuts at: 2721 3022 3911 5540 8
Size: 301 889 1629 43
Fragments arranged by size:
1629 1425 889 477 301 275 237 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2244 3022 1537
Size: 275 432 778 4090
Fragments arranged by size:
4090 778 432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3505 1353
Size: 2152 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2209
Size: 5575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2313 2326 3638 4235 5022 5290
Size: 393 611 70 13 1312 597 787 268
Cuts at: 5290 1239
Size: 1524
Fragments arranged by size:
1524 1312 787 611 597 393 268 70
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2375
Size: 5575
EcoO109I rG'GnC_Cy
Cuts at: 2052 2173 2218 2239 2052
Size: 121 45 21 5388
Fragments arranged by size:
5388 121 45 21
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
EcoRV GAT'ATC
Cuts at: 2302 2455 2302
Size: 153 5422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2642 2806 2878 2642
Size: 164 72 5339
Fragments arranged by size:
5339 164 72
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2286 2318 2324 2356 2582
Size: 32 135 32 714 32 6 32 226
Cuts at: 2582 2614 4216 4248 4290 4322 1373
Size: 32 1602 32 42 32 2626
Fragments arranged by size:
2626 1602 714 226 135 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2757 4689 4873 1305
Size: 874 578 1932 184 2007
Fragments arranged by size:
2007 1932 874 578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 2862
Fragments arranged by size:
2862 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2047 2291 2991 3668 4816 5061 1606
Size: 434 7 244 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 434 245 244 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 2239
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2291 2291
Size: 5575
SacI G_AGCT'C
Cuts at: 2211 2211
Size: 5575
SacII CC_GC'GG
Cuts at: 2041 2041
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SexAI A'CCwGG_T
Cuts at: 2871 2871
Size: 5575
SfcI C'TryA_G
Cuts at: 826 1045 2923 4058 4739 4930 826
Size: 219 1878 1135 681 191 1471
Fragments arranged by size:
1878 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2252 2252
Size: 5575
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 2267 2585 1700
Size: 567 318 4690
Fragments arranged by size:
4690 567 318
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2282 1517
Size: 765 4810
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 3014 513
Size: 552 939 165 845 3074
Fragments arranged by size:
3074 939 845 552 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2546 3602 3619 3772 3957 4402
Size: 1255 810 156 1056 17 153 185 445
Cuts at: 4402 325
Size: 1498
Fragments arranged by size:
1498 1255 1056 810 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 3821 5533
Size: 274 80 33 51 1443 129 1715 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1715 1712 1443 274 138 129 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 2228 3704 2228
Size: 1476 4099
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI
SfoI SrfI SwaI XcmI