(Linear) (Six-base) MAPSORT of: KIBB7566.seq Check: 5158 from: 1 to: 831 KIBB7566.orf With 182 enzymes: SgfI * March 22, 2012 15:37 .. AatII G_ACGT'C Cuts at: 0 82 831 Size: 82 749 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 831 Size: 694 137 ApaI G_GGCC'C Cuts at: 0 284 526 831 Size: 284 242 305 Fragments arranged by size: 305 284 242 AscI GG'CGCG_CC Cuts at: 0 371 831 Size: 371 460 AvaI C'yCGr_G Cuts at: 0 15 160 465 831 Size: 15 145 305 366 Fragments arranged by size: 366 305 145 15 BanI G'GyrC_C Cuts at: 0 123 335 831 Size: 123 212 496 Fragments arranged by size: 496 212 123 BanII G_rGCy'C Cuts at: 0 254 284 526 831 Size: 254 30 242 305 Fragments arranged by size: 305 254 242 30 BbeI G_GCGC'C Cuts at: 0 127 339 831 Size: 127 212 492 Fragments arranged by size: 492 212 127 BbsI GAAGACnn'nnnn_ Cuts at: 0 82 831 Size: 82 749 BglI GCCn_nnn'nGGC Cuts at: 0 46 295 806 831 Size: 46 249 511 25 Fragments arranged by size: 511 249 46 25 BglII A'GATC_T Cuts at: 0 476 831 Size: 476 355 BlpI GC'TnA_GC Cuts at: 0 31 831 Size: 31 800 Bme1580I G_kGCm'C Cuts at: 0 42 284 526 831 Size: 42 242 242 305 Fragments arranged by size: 305 242 242 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 399 508 831 Size: 399 109 323 Fragments arranged by size: 399 323 109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 748 831 Size: 748 83 BsaHI Gr'CG_yC Cuts at: 0 79 124 336 419 634 662 824 831 Size: 79 45 212 83 215 28 162 7 Fragments arranged by size: 215 212 162 83 79 45 28 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 619 649 831 Size: 619 30 182 Fragments arranged by size: 619 182 30 BseYI C'CCAG_C Cuts at: 0 132 552 831 Size: 132 420 279 Fragments arranged by size: 420 279 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 385 831 Size: 40 345 446 Fragments arranged by size: 446 345 40 BsiEI CG_ry'CG Cuts at: 0 113 290 413 831 Size: 113 177 123 418 Fragments arranged by size: 418 177 123 113 BsmBI CGTCTCn'nnnn_ Cuts at: 0 544 831 Size: 544 287 Bsp1286I G_dGCh'C Cuts at: 0 42 254 284 526 831 Size: 42 212 30 242 305 Fragments arranged by size: 305 242 212 42 30 BsrBI CCG'CTC Cuts at: 0 159 831 Size: 159 672 BsrFI r'CCGG_y Cuts at: 0 112 194 265 412 716 831 Size: 112 82 71 147 304 115 Fragments arranged by size: 304 147 115 112 82 71 BssHII G'CGCG_C Cuts at: 0 146 298 371 531 533 831 Size: 146 152 73 160 2 298 Fragments arranged by size: 298 160 152 146 73 2 BstXI CCAn_nnnn'nTGG Cuts at: 0 318 516 831 Size: 318 198 315 Fragments arranged by size: 318 315 198 BstYI r'GATC_y Cuts at: 0 476 831 Size: 476 355 BtgI C'CryG_G Cuts at: 0 200 284 831 Size: 200 84 547 Fragments arranged by size: 547 200 84 BtsI GCAGTG_nn' Cuts at: 0 42 831 Size: 42 789 EaeI y'GGCC_r Cuts at: 0 47 65 110 287 320 410 638 831 Size: 47 18 45 177 33 90 228 193 Fragments arranged by size: 228 193 177 90 47 45 33 18 EagI C'GGCC_G Cuts at: 0 110 287 410 831 Size: 110 177 123 421 Fragments arranged by size: 421 177 123 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 252 640 831 Size: 252 388 191 Fragments arranged by size: 388 252 191 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 399 508 694 831 Size: 399 109 186 137 Fragments arranged by size: 399 186 137 109 EcoNI CCTnn'n_nnAGG Cuts at: 0 427 831 Size: 427 404 EcoO109I rG'GnC_Cy Cuts at: 0 138 164 219 384 831 Size: 138 26 55 165 447 Fragments arranged by size: 447 165 138 55 26 FseI GG_CCGG'CC Cuts at: 0 198 831 Size: 198 633 HaeII r_GCGC'y Cuts at: 0 127 339 793 831 Size: 127 212 454 38 Fragments arranged by size: 454 212 127 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 619 651 718 750 831 Size: 619 32 67 32 81 Fragments arranged by size: 619 81 67 32 32 KasI G'GCGC_C Cuts at: 0 123 335 831 Size: 123 212 496 Fragments arranged by size: 496 212 123 MspA1I CmG'CkG Cuts at: 0 45 286 292 831 Size: 45 241 6 539 Fragments arranged by size: 539 241 45 6 NaeI GCC'GGC Cuts at: 0 114 196 267 414 831 Size: 114 82 71 147 417 Fragments arranged by size: 417 147 114 82 71 NarI GG'CG_CC Cuts at: 0 124 336 831 Size: 124 212 495 Fragments arranged by size: 495 212 124 NgoMIV G'CCGG_C Cuts at: 0 112 194 265 412 831 Size: 112 82 71 147 419 Fragments arranged by size: 419 147 112 82 71 NotI GC'GGCC_GC Cuts at: 0 287 831 Size: 287 544 PflMI CCAn_nnn'nTGG Cuts at: 0 350 831 Size: 350 481 PfoI T'CCnGG_A Cuts at: 0 422 831 Size: 422 409 PpuMI rG'GwC_Cy Cuts at: 0 164 384 831 Size: 164 220 447 Fragments arranged by size: 447 220 164 PshAI GACnn'nnGTC Cuts at: 0 389 831 Size: 389 442 PspOMI G'GGCC_C Cuts at: 0 280 522 831 Size: 280 242 309 Fragments arranged by size: 309 280 242 PstI C_TGCA'G Cuts at: 0 104 831 Size: 104 727 SacII CC_GC'GG Cuts at: 0 287 831 Size: 287 544 SanDI GG'GwC_CC Cuts at: 0 164 384 831 Size: 164 220 447 Fragments arranged by size: 447 220 164 SfcI C'TryA_G Cuts at: 0 100 831 Size: 100 731 SfoI GGC'GCC Cuts at: 0 125 337 831 Size: 125 212 494 Fragments arranged by size: 494 212 125 SgrAI Cr'CCGG_yG Cuts at: 0 265 831 Size: 265 566 SmaI CCC'GGG Cuts at: 0 17 831 Size: 17 814 SmlI C'TyrA_G Cuts at: 0 763 831 Size: 763 68 SrfI GCCC'GGGC Cuts at: 0 17 831 Size: 17 814 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 577 831 Size: 577 254 XmaI C'CCGG_G Cuts at: 0 15 831 Size: 15 816 XmnI GAAnn'nnTTC Cuts at: 0 496 831 Size: 496 335 ZraI GAC'GTC Cuts at: 0 80 831 Size: 80 751 Enzymes that do cut: AatII AcuI ApaI AscI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BpmI BpuEI BsaHI BsaXI BseYI BsgI BsiEI BsmBI Bsp1286I BsrBI BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV NotI PflMI PfoI PpuMI PshAI PspOMI PstI SacII SanDI SfcI SfoI SgrAI SmaI SmlI SrfI TaqII XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI