################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AlwNI Aor51HI AscI AseI AsnI Asp700I AviII AvrII BcgI BciVI BclI BfaI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EarI Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I MaeI MamI MfeI MluI MroI MslI MunI NdeI NheI NruI NsiI NspI PacI PaeR7I PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1076 GGTACC G^GTACC AcsI 1645 GAATTT [GA]^AATT[CT] Alw44I 817 GTGCAC G^TGCAC AocI 1231 CCTGAGG CC^T[ACGT]AGG ApaLI 817 GTGCAC G^TGCAC ApoI 1645 GAATTT [GA]^AATT[CT] Asp718I 1076 GGTACC G^GTACC BalI 1344 TGGCCA TGG^CCA BamHI 101 GGATCC G^GATCC BbrPI 1726 CACGTG CAC^GTG BbsI 1140 GTCTTC GAAGAC(2/6) BbvCI 495 CCTCAGC CCTCAGC(-5/-2) BglII 761 AGATCT A^GATCT BlpI 552 GCTGAGC GC^T[ACGT]AGC BpmI 829 CTGGAG CTGGAG(16/14) Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC BpuAI 1140 GTCTTC GAAGAC(2/6) BstBI 361 TTCGAA TT^CGAA BstEII 1330 GGTGACC G^GT[ACGT]ACC BstPI 1330 GGTGACC G^GT[ACGT]ACC Bsu36I 1231 CCTGAGG CC^T[ACGT]AGG CelII 552 GCTGAGC GC^T[ACGT]AGC Eco81I 1231 CCTGAGG CC^T[ACGT]AGG EcoNI 1281 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1330 GGTGACC G^GT[ACGT]ACC KpnI 1076 GGTACC GGTAC^C MluNI 1344 TGGCCA TGG^CCA MscI 1344 TGGCCA TGG^CCA NotI 513 GCGGCCGC GC^GGCCGC NspV 361 TTCGAA TT^CGAA PflMI 1011 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1726 CACGTG CAC^GTG PmlI 1726 CACGTG CAC^GTG PshAI 1528 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfcI 768 CTACAG C^T[GA][CT]AG SfiI 130 GGCCTCTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 361 TTCGAA TT^CGAA SgrAI 1094 CGCCGGCG C[GA]^CCGG[CT]G Tsp509I 1646 AATT ^AATT TspRI 1254 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 1011 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 401 ACACGT A^C[GA][CT]GT 1725 ACACGT AhdI 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 907 GACCGGCCGTC ApaI 596 GGGCCC GGGCC^C 722 GGGCCC AspEI 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 907 GACCGGCCGTC AspI 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1602 GACTACGTC BglI 131 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1560 GCCGTCTTGGC BsaAI 402 CACGTA [CT]AC^GT[GA] 1726 CACGTG BsaI 283 GAGACC GGTCTC(1/5) 1581 GGTCTC BsrBI 85 GAGCGG CCGCTC(-3/-3) 1241 GAGCGG BsrI 1253 CCAGT ACTGG(1/-1) 1758 CCAGT Eam1105I 892 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 907 GACCGGCCGTC HincII 49 GTCAAC GT[CT]^[GA]AC 184 GTCAAC HindII 49 GTCAAC GT[CT]^[GA]AC 184 GTCAAC KspI 264 CCGCGG CCGC^GG 742 CCGCGG MaeIII 67 GTCAC ^GT[ACGT]AC 1331 GTGAC MboII 1674 GAAGA GAAGA(8/7) 1141 TCTTC MseI 1664 TTAA T^TAA 1753 TTAA NcoI 195 CCATGG C^CATGG 1494 CCATGG NlaIII 196 CATG CATG^ 1495 CATG PflFI 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1602 GACTACGTC PpuMI 737 AGGTCCC [GA]G^G[AT]CC[CT] 875 AGGACCC PvuII 235 CAGCTG CAG^CTG 1224 CAGCTG SacI 631 GAGCTC GAGCT^C 1747 GAGCTC SacII 264 CCGCGG CCGC^GG 742 CCGCGG SmaI 480 CCCGGG CCC^GGG 902 CCCGGG TaqI 170 TCGA T^CGA 362 TCGA Tru9I 1664 TTAA T^TAA 1753 TTAA Tsp45I 67 GTCAC ^GT[GC]AC 1331 GTGAC Tth111I 1474 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1602 GACTACGTC TthHB8I 170 TCGA T^CGA 362 TCGA XmaCI 480 CCCGGG C^CCGGG 902 CCCGGG XmaI 480 CCCGGG C^CCGGG 902 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssHII BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI StyI TseI XhoII