(Circular) (Six-base) MAPSORT of: pFN21AE5065.seq Check: 9713 from: 1 to: 6475 pFN21AE5065 6475 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 3081 3796 3081 Size: 715 5760 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3029 4538 5922 6190 1632 Size: 1397 1509 1384 268 1917 Fragments arranged by size: 1917 1509 1397 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 2406 3730 5374 6351 1203 Size: 1203 1324 1644 977 1327 Fragments arranged by size: 1644 1327 1324 1203 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 2902 2917 5204 2902 Size: 15 2287 4173 Fragments arranged by size: 4173 2287 15 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AlwNI CAG_nnn'CTG Cuts at: 4218 5790 4218 Size: 1572 4903 ApaI G_GGCC'C Cuts at: 2605 2731 2605 Size: 126 6349 ApaLI G'TGCA_C Cuts at: 2822 4532 5688 2822 Size: 1710 1156 3609 Fragments arranged by size: 3609 1710 1156 ApoI r'AATT_y Cuts at: 1493 3650 3784 4010 4162 6271 1493 Size: 2157 134 226 152 2109 1697 Fragments arranged by size: 2157 2109 1697 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2285 2300 2485 2885 2907 3800 Size: 73 270 72 15 185 400 22 893 Cuts at: 3800 1870 Size: 4545 Fragments arranged by size: 4545 893 400 270 185 73 72 22 15 BamHI G'GATC_C Cuts at: 2106 3805 4172 2106 Size: 1699 367 4409 Fragments arranged by size: 4409 1699 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2372 2422 2490 3081 3103 Size: 325 217 627 585 50 68 591 22 Cuts at: 3103 3397 3796 5251 618 Size: 294 399 1455 1842 Fragments arranged by size: 1842 1455 627 591 585 399 325 294 217 68 50 22 BanII G_rGCy'C Cuts at: 1391 2212 2564 2605 2640 2673 2731 2756 2841 Size: 821 352 41 35 33 58 25 85 Cuts at: 2841 3045 3756 3794 1391 Size: 204 711 38 4072 Fragments arranged by size: 4072 821 711 352 204 85 58 41 38 35 33 25 BbeI G_GCGC'C Cuts at: 2376 2494 3107 2376 Size: 118 613 5744 Fragments arranged by size: 5744 613 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3138 928 Size: 971 1239 4265 Fragments arranged by size: 4265 1239 971 BbvCI CC'TCA_GC Cuts at: 2501 2501 Size: 6475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 3571 5086 Size: 107 122 71 631 605 470 1429 1515 Cuts at: 5086 136 Size: 1525 Fragments arranged by size: 1525 1515 1429 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 2766 6470 2766 Size: 3704 2771 BlpI GC'TnA_GC Cuts at: 2558 3892 2558 Size: 1334 5141 Bme1580I G_kGCm'C Cuts at: 1790 2427 2605 2731 2826 4536 5692 1790 Size: 637 178 126 95 1710 1156 2573 Fragments arranged by size: 2573 1710 1156 637 178 126 95 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3757 4206 5164 Size: 416 53 83 399 24 2340 449 958 Cuts at: 5164 442 Size: 1753 Fragments arranged by size: 2340 1753 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 5135 1239 Size: 1616 2280 2579 Fragments arranged by size: 2579 2280 1616 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 3592 5138 882 Size: 617 783 1310 1546 2219 Fragments arranged by size: 2219 1546 1310 783 617 BsaAI yAC'GTr Cuts at: 493 1204 2409 3733 4266 493 Size: 711 1205 1324 533 2702 Fragments arranged by size: 2702 1324 1205 711 533 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 2464 2491 3104 Size: 53 83 186 936 840 91 27 613 Cuts at: 3104 3320 4282 4664 5299 275 Size: 216 962 382 635 1451 Fragments arranged by size: 1451 962 936 840 635 613 382 216 186 91 83 53 27 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2787 2817 1178 Size: 30 165 30 1384 30 4836 Fragments arranged by size: 4836 1384 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2666 2710 2732 1186 Size: 1045 435 44 22 4929 Fragments arranged by size: 4929 1045 435 44 22 BseYI C'CCAG_C Cuts at: 2242 2427 2587 2614 2850 5678 6174 2242 Size: 185 160 27 236 2828 496 2543 Fragments arranged by size: 2828 2543 496 236 185 160 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2104 3292 3404 1163 Size: 573 368 1188 112 4234 Fragments arranged by size: 4234 1188 573 368 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2522 2918 2976 3925 4686 5714 Size: 275 186 521 396 58 949 761 1028 Cuts at: 5714 1540 Size: 2301 Fragments arranged by size: 2301 1028 949 761 521 396 275 186 58 BsiHKAI G_wGCw'C Cuts at: 2535 2555 2640 2826 3039 3756 4536 4621 5692 Size: 20 85 186 213 717 780 85 1071 Cuts at: 5692 2535 Size: 3318 Fragments arranged by size: 3318 1071 780 717 213 186 85 85 20 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2212 2427 2535 2555 2564 2605 2640 Size: 399 422 215 108 20 9 41 35 Cuts at: 2640 2673 2731 2756 2826 2841 3039 3045 3756 Size: 33 58 25 70 15 198 6 711 Cuts at: 3756 3794 4536 4621 5692 1391 Size: 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 422 399 215 198 108 85 70 58 41 38 35 33 25 20 15 9 6 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 2731 2994 3093 3830 844 Size: 1194 693 263 99 737 3489 Fragments arranged by size: 3489 1194 737 693 263 99 BsrBI CCG'CTC Cuts at: 1394 1823 2092 3248 4362 1394 Size: 429 269 1156 1114 3507 Fragments arranged by size: 3507 1156 1114 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2025 2032 2913 2971 2975 3100 Size: 110 208 7 7 881 58 4 125 Cuts at: 3100 3125 3322 3517 5119 5306 1700 Size: 25 197 195 1602 187 2869 Fragments arranged by size: 2869 1602 881 208 197 195 187 125 110 58 25 7 7 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 2014 2061 2925 1925 Size: 89 47 864 5475 Fragments arranged by size: 5475 864 89 47 BssSI C'ACGA_G Cuts at: 2318 3038 3589 4535 5547 2318 Size: 720 551 946 1012 3246 Fragments arranged by size: 3246 1012 946 720 551 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 2367 5279 2367 Size: 2912 3563 BstEII G'GTnAC_C Cuts at: 1184 3335 1184 Size: 2151 4324 BstYI r'GATC_y Cuts at: 1972 2106 2766 2808 3000 3495 3805 4172 4557 Size: 134 660 42 192 495 310 367 385 Cuts at: 4557 4574 6026 6112 6124 6470 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 660 495 385 367 346 310 192 134 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3237 3237 Size: 6475 BtgI C'CryG_G Cuts at: 513 1065 2004 2200 2269 2673 2747 3352 3471 Size: 552 939 196 69 404 74 605 119 Cuts at: 3471 3499 513 Size: 28 3489 Fragments arranged by size: 3489 939 605 552 404 196 119 74 69 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 3991 4785 4805 3991 Size: 794 20 5661 Fragments arranged by size: 5661 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3714 3749 529 Size: 35 3150 35 3255 Fragments arranged by size: 3255 3150 35 35 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2519 2682 Size: 54 1425 50 275 18 373 316 163 Cuts at: 2682 2915 2973 3349 3468 3515 3544 3922 4811 Size: 233 58 376 119 47 29 378 889 Cuts at: 4811 6440 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 378 376 373 316 275 233 163 119 58 54 50 47 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2519 2915 2973 3922 1537 Size: 275 707 396 58 949 4090 Fragments arranged by size: 4090 949 707 396 275 58 EarI CTCTTCn'nnn_ Cuts at: 1353 4405 1353 Size: 3052 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2501 2993 3152 5062 5436 5582 Size: 1064 138 912 492 159 1910 374 146 Cuts at: 5582 387 Size: 1280 Fragments arranged by size: 1910 1280 1064 912 492 374 159 146 138 EcoICRI GAG'CTC Cuts at: 2638 3754 2638 Size: 1116 5359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2855 3029 4538 5135 5922 6190 1239 Size: 393 1223 174 1509 597 787 268 1524 Fragments arranged by size: 1524 1509 1223 787 597 393 268 174 EcoNI CCTnn'n_nnAGG Cuts at: 3290 3290 Size: 6475 EcoO109I rG'GnC_Cy Cuts at: 2601 2727 2728 2743 2857 2881 2601 Size: 126 1 15 114 24 6195 Fragments arranged by size: 6195 126 114 24 15 1 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 2376 2494 3107 3368 5622 1678 Size: 698 118 613 261 2254 2531 Fragments arranged by size: 2531 2254 698 613 261 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2787 2819 2888 2903 2920 Size: 32 135 32 1215 32 69 15 17 Cuts at: 2920 2935 3284 3316 3655 3687 5116 5148 5190 Size: 15 349 32 339 32 1429 32 42 Cuts at: 5190 5222 1373 Size: 32 2626 Fragments arranged by size: 2626 1429 1215 349 339 135 69 42 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2056 2191 3819 4065 669 Size: 988 281 118 135 1628 246 3079 Fragments arranged by size: 3079 1628 988 281 246 135 118 HindIII A'AGCT_T Cuts at: 748 748 Size: 6475 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KasI G'GCGC_C Cuts at: 2372 2490 3103 2372 Size: 118 613 5744 Fragments arranged by size: 5744 613 118 KpnI G_GTAC'C Cuts at: 3085 3800 3085 Size: 715 5760 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3417 3629 5589 5773 1305 Size: 2112 212 1960 184 2007 Fragments arranged by size: 2112 2007 1960 212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3351 10 Size: 54 1425 343 1519 3134 Fragments arranged by size: 3134 1519 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4433 4792 4951 518 Size: 648 3267 359 159 2042 Fragments arranged by size: 3267 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2242 2271 2329 2392 2622 2749 3231 Size: 558 78 29 58 63 230 127 482 Cuts at: 3231 3891 4568 5716 5961 1606 Size: 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 558 482 245 230 127 78 63 58 29 NaeI GCC'GGC Cuts at: 2020 2027 2973 2977 3102 3324 3519 2020 Size: 7 946 4 125 222 195 4976 Fragments arranged by size: 4976 946 222 195 125 7 4 NarI GG'CG_CC Cuts at: 2373 2491 3104 2373 Size: 118 613 5744 Fragments arranged by size: 5744 613 118 NcoI C'CATG_G Cuts at: 513 1065 2004 2200 3499 513 Size: 552 939 196 1299 3489 Fragments arranged by size: 3489 1299 939 552 196 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NgoMIV G'CCGG_C Cuts at: 2018 2025 2971 2975 3100 3322 3517 2018 Size: 7 946 4 125 222 195 4976 Fragments arranged by size: 4976 946 222 195 125 7 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 2519 3922 2519 Size: 1403 5072 NspI r_CATG'y Cuts at: 1132 1229 3833 5378 1132 Size: 97 2604 1545 2229 Fragments arranged by size: 2604 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2598 2785 3190 1348 Size: 1250 187 405 4633 Fragments arranged by size: 4633 1250 405 187 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 3022 1108 Size: 1914 4561 PfoI T'CCnGG_A Cuts at: 2400 2400 Size: 6475 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PmlI CAC'GTG Cuts at: 3733 3733 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2743 2881 2743 Size: 138 6337 PshAI GACnn'nnGTC Cuts at: 1613 3537 1613 Size: 1924 4551 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PspOMI G'GGCC_C Cuts at: 2601 2727 2601 Size: 126 6349 PstI C_TGCA'G Cuts at: 830 3827 830 Size: 2997 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2242 3231 2242 Size: 989 5486 SacI G_AGCT'C Cuts at: 2640 3756 2640 Size: 1116 5359 SacII CC_GC'GG Cuts at: 2272 2750 2272 Size: 478 5997 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2773 3823 4958 5639 5830 826 Size: 219 1728 1050 1135 681 191 1471 Fragments arranged by size: 1728 1471 1135 1050 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2142 2142 Size: 6475 SfoI GGC'GCC Cuts at: 2374 2492 3105 2374 Size: 118 613 5744 Fragments arranged by size: 5744 613 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 3100 1700 Size: 1400 5075 SmaI CCC'GGG Cuts at: 1872 2487 2909 3802 1872 Size: 615 422 893 4545 Fragments arranged by size: 4545 893 615 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 5480 5742 820 Size: 197 926 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6475 StyI C'CwwG_G Cuts at: 513 1065 2004 2200 3499 3509 3914 513 Size: 552 939 196 1299 10 405 3074 Fragments arranged by size: 3074 1299 939 552 405 196 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2410 4502 4519 4672 4857 5302 325 Size: 1255 830 2092 17 153 185 445 1498 Fragments arranged by size: 2092 1498 1255 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 3482 3610 1316 Size: 2166 128 4181 Fragments arranged by size: 4181 2166 128 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 2485 2907 3800 1870 Size: 615 422 893 4545 Fragments arranged by size: 4545 893 615 422 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI XcmI