(Linear) (Six-base) MAPSORT of: KIEE5065.seq Check: 9694 from: 1 to: 1770 KIEE5065.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. Acc65I G'GTAC_C Cuts at: 0 1076 1770 Size: 1076 694 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1024 1770 Size: 1024 746 AflIII A'CryG_T Cuts at: 0 401 1725 1770 Size: 401 1324 45 Fragments arranged by size: 1324 401 45 AhdI GACnn_n'nnGTC Cuts at: 0 897 912 1770 Size: 897 15 858 Fragments arranged by size: 897 858 15 ApaI G_GGCC'C Cuts at: 0 600 726 1770 Size: 600 126 1044 Fragments arranged by size: 1044 600 126 ApaLI G'TGCA_C Cuts at: 0 817 1770 Size: 817 953 ApoI r'AATT_y Cuts at: 0 1645 1770 Size: 1645 125 AvaI C'yCGr_G Cuts at: 0 208 280 295 480 880 902 1770 Size: 208 72 15 185 400 22 868 Fragments arranged by size: 868 400 208 185 72 22 15 BamHI G'GATC_C Cuts at: 0 101 1770 Size: 101 1669 BanI G'GyrC_C Cuts at: 0 367 417 485 1076 1098 1392 1770 Size: 367 50 68 591 22 294 378 Fragments arranged by size: 591 378 367 294 68 50 22 BanII G_rGCy'C Cuts at: 0 207 559 600 635 668 726 751 836 Size: 207 352 41 35 33 58 25 85 Cuts at: 836 1040 1751 1770 Size: 204 711 19 Fragments arranged by size: 711 352 207 204 85 58 41 35 33 25 19 BbeI G_GCGC'C Cuts at: 0 371 489 1102 1770 Size: 371 118 613 668 Fragments arranged by size: 668 613 371 118 BbsI GAAGACnn'nnnn_ Cuts at: 0 1133 1770 Size: 1133 637 BbvCI CC'TCA_GC Cuts at: 0 496 1770 Size: 496 1274 BglI GCCn_nnn'nGGC Cuts at: 0 137 1566 1770 Size: 137 1429 204 Fragments arranged by size: 1429 204 137 BglII A'GATC_T Cuts at: 0 761 1770 Size: 761 1009 BlpI GC'TnA_GC Cuts at: 0 553 1770 Size: 553 1217 Bme1580I G_kGCm'C Cuts at: 0 422 600 726 821 1770 Size: 422 178 126 95 949 Fragments arranged by size: 949 422 178 126 95 BmrI ACTGGGnnnn_n' Cuts at: 0 1752 1770 Size: 1752 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1770 Size: 850 920 Bpu10I CC'TnA_GC Cuts at: 0 496 1770 Size: 496 1274 BsaI GGTCTCn'nnnn_ Cuts at: 0 277 1587 1770 Size: 277 1310 183 Fragments arranged by size: 1310 277 183 BsaAI yAC'GTr Cuts at: 0 404 1728 1770 Size: 404 1324 42 Fragments arranged by size: 1324 404 42 BsaHI Gr'CG_yC Cuts at: 0 368 459 486 1099 1315 1770 Size: 368 91 27 613 216 455 Fragments arranged by size: 613 455 368 216 91 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 782 812 1770 Size: 782 30 958 Fragments arranged by size: 958 782 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 226 661 705 727 1770 Size: 226 435 44 22 1043 Fragments arranged by size: 1043 435 226 44 22 BseYI C'CCAG_C Cuts at: 0 237 422 582 609 845 1770 Size: 237 185 160 27 236 925 Fragments arranged by size: 925 237 236 185 160 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 1287 1399 1770 Size: 99 1188 112 371 Fragments arranged by size: 1188 371 112 99 BsiEI CG_ry'CG Cuts at: 0 517 913 971 1770 Size: 517 396 58 799 Fragments arranged by size: 799 517 396 58 BsiHKAI G_wGCw'C Cuts at: 0 530 550 635 821 1034 1751 1770 Size: 530 20 85 186 213 717 19 Fragments arranged by size: 717 530 213 186 85 20 19 Bsp1286I G_dGCh'C Cuts at: 0 207 422 530 550 559 600 635 668 Size: 207 215 108 20 9 41 35 33 Cuts at: 668 726 751 821 836 1034 1040 1751 1770 Size: 58 25 70 15 198 6 711 19 Fragments arranged by size: 711 215 207 198 108 70 58 41 35 33 25 20 19 15 9 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 33 726 989 1088 1770 Size: 33 693 263 99 682 Fragments arranged by size: 693 682 263 99 33 BsrBI CCG'CTC Cuts at: 0 87 1243 1770 Size: 87 1156 527 Fragments arranged by size: 1156 527 87 BsrFI r'CCGG_y Cuts at: 0 13 20 27 908 966 970 1095 1120 Size: 13 7 7 881 58 4 125 25 Cuts at: 1120 1317 1512 1770 Size: 197 195 258 Fragments arranged by size: 881 258 197 195 125 58 25 13 7 7 4 BssHII G'CGCG_C Cuts at: 0 9 56 920 1770 Size: 9 47 864 850 Fragments arranged by size: 864 850 47 9 BssSI C'ACGA_G Cuts at: 0 313 1033 1584 1770 Size: 313 720 551 186 Fragments arranged by size: 720 551 313 186 BstBI TT'CG_AA Cuts at: 0 362 1770 Size: 362 1408 BstEII G'GTnAC_C Cuts at: 0 1330 1770 Size: 1330 440 BstYI r'GATC_y Cuts at: 0 101 761 803 995 1490 1770 Size: 101 660 42 192 495 280 Fragments arranged by size: 660 495 280 192 101 42 Bsu36I CC'TnA_GG Cuts at: 0 1232 1770 Size: 1232 538 BtgI C'CryG_G Cuts at: 0 195 264 668 742 1347 1466 1494 1770 Size: 195 69 404 74 605 119 28 276 Fragments arranged by size: 605 404 276 195 119 74 69 28 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1709 1744 1770 Size: 1709 35 26 Fragments arranged by size: 1709 35 26 EaeI y'GGCC_r Cuts at: 0 198 514 677 910 968 1344 1463 1510 Size: 198 316 163 233 58 376 119 47 Cuts at: 1510 1539 1770 Size: 29 231 Fragments arranged by size: 376 316 233 231 198 163 119 58 47 29 EagI C'GGCC_G Cuts at: 0 514 910 968 1770 Size: 514 396 58 802 Fragments arranged by size: 802 514 396 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 496 988 1147 1770 Size: 496 492 159 623 Fragments arranged by size: 623 496 492 159 EcoICRI GAG'CTC Cuts at: 0 633 1749 1770 Size: 633 1116 21 Fragments arranged by size: 1116 633 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1024 1770 Size: 850 174 746 Fragments arranged by size: 850 746 174 EcoNI CCTnn'n_nnAGG Cuts at: 0 1285 1770 Size: 1285 485 EcoO109I rG'GnC_Cy Cuts at: 0 596 722 723 738 852 876 1770 Size: 596 126 1 15 114 24 894 Fragments arranged by size: 894 596 126 114 24 15 1 HaeII r_GCGC'y Cuts at: 0 371 489 1102 1363 1770 Size: 371 118 613 261 407 Fragments arranged by size: 613 407 371 261 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 782 814 883 898 915 930 1279 1311 Size: 782 32 69 15 17 15 349 32 Cuts at: 1311 1650 1682 1770 Size: 339 32 88 Fragments arranged by size: 782 349 339 88 69 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 0 51 186 1770 Size: 51 135 1584 Fragments arranged by size: 1584 135 51 KasI G'GCGC_C Cuts at: 0 367 485 1098 1770 Size: 367 118 613 672 Fragments arranged by size: 672 613 367 118 KpnI G_GTAC'C Cuts at: 0 1080 1770 Size: 1080 690 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1412 1624 1770 Size: 1412 212 146 Fragments arranged by size: 1412 212 146 MscI TGG'CCA Cuts at: 0 1346 1770 Size: 1346 424 MspA1I CmG'CkG Cuts at: 0 159 237 266 324 387 617 744 1226 Size: 159 78 29 58 63 230 127 482 Cuts at: 1226 1770 Size: 544 Fragments arranged by size: 544 482 230 159 127 78 63 58 29 NaeI GCC'GGC Cuts at: 0 15 22 968 972 1097 1319 1514 1770 Size: 15 7 946 4 125 222 195 256 Fragments arranged by size: 946 256 222 195 125 15 7 4 NarI GG'CG_CC Cuts at: 0 368 486 1099 1770 Size: 368 118 613 671 Fragments arranged by size: 671 613 368 118 NcoI C'CATG_G Cuts at: 0 195 1494 1770 Size: 195 1299 276 Fragments arranged by size: 1299 276 195 NgoMIV G'CCGG_C Cuts at: 0 13 20 966 970 1095 1317 1512 1770 Size: 13 7 946 4 125 222 195 258 Fragments arranged by size: 946 258 222 195 125 13 7 4 NotI GC'GGCC_GC Cuts at: 0 514 1770 Size: 514 1256 PasI CC'CwG_GG Cuts at: 0 593 780 1185 1770 Size: 593 187 405 585 Fragments arranged by size: 593 585 405 187 PflMI CCAn_nnn'nTGG Cuts at: 0 1017 1770 Size: 1017 753 PfoI T'CCnGG_A Cuts at: 0 395 1770 Size: 395 1375 PmlI CAC'GTG Cuts at: 0 1728 1770 Size: 1728 42 PpuMI rG'GwC_Cy Cuts at: 0 738 876 1770 Size: 738 138 894 Fragments arranged by size: 894 738 138 PshAI GACnn'nnGTC Cuts at: 0 1532 1770 Size: 1532 238 PspOMI G'GGCC_C Cuts at: 0 596 722 1770 Size: 596 126 1048 Fragments arranged by size: 1048 596 126 PvuII CAG'CTG Cuts at: 0 237 1226 1770 Size: 237 989 544 Fragments arranged by size: 989 544 237 SacI G_AGCT'C Cuts at: 0 635 1751 1770 Size: 635 1116 19 Fragments arranged by size: 1116 635 19 SacII CC_GC'GG Cuts at: 0 267 745 1770 Size: 267 478 1025 Fragments arranged by size: 1025 478 267 SfcI C'TryA_G Cuts at: 0 768 1770 Size: 768 1002 SfiI GGCCn_nnn'nGGCC Cuts at: 0 137 1770 Size: 137 1633 SfoI GGC'GCC Cuts at: 0 369 487 1100 1770 Size: 369 118 613 670 Fragments arranged by size: 670 613 369 118 SgrAI Cr'CCGG_yG Cuts at: 0 1095 1770 Size: 1095 675 SmaI CCC'GGG Cuts at: 0 482 904 1770 Size: 482 422 866 Fragments arranged by size: 866 482 422 StyI C'CwwG_G Cuts at: 0 195 1494 1504 1770 Size: 195 1299 10 266 Fragments arranged by size: 1299 266 195 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 405 1770 Size: 405 1365 Tth111I GACn'n_nGTC Cuts at: 0 1477 1605 1770 Size: 1477 128 165 Fragments arranged by size: 1477 165 128 XmaI C'CCGG_G Cuts at: 0 480 902 1770 Size: 480 422 868 Fragments arranged by size: 868 480 422 Enzymes that do cut: Acc65I AcuI AflIII AhdI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI Bsu36I BtgI CspCI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PvuII SacI SacII SfcI SfiI SfoI SgrAI SmaI StyI TaqII Tth111I XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstXI BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI MslI NdeI NheI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XhoI XmnI ZraI