################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsiWI BsmAI BsmBI BsmI BspDI BspHI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MaeIII MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TfiI Tsp45I Tsp509I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 774 TCCGGA T^CCGGA AflIII 494 ACATGT A^C[GA][CT]GT AhdI 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 131 GGGCCC GGGCC^C AspEI 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 10 GGCGCC GGCGC^C BbrPI 957 CACGTG CAC^GTG BglI 74 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 779 AGATCT A^GATCT BsaAI 957 CACGTG [CT]AC^GT[GA] BsaWI 774 TCCGGA [AT]^CCGG[AT] BseAI 774 TCCGGA T^CCGGA Bsp1407I 584 TGTACA T^GTACA BspEI 774 TCCGGA T^CCGGA BspLU11I 494 ACATGT A^CATGT BsrDI 708 CATTGC GCAATG(2/0) BsrGI 584 TGTACA T^GTACA BstXI 965 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 965 CCAAGG C^C[AT][AT]GG HincII 796 GTCAAC GT[CT]^[GA]AC HindII 796 GTCAAC GT[CT]^[GA]AC KasI 10 GGCGCC G^GCGCC KspI 35 CCGCGG CCGC^GG MroI 774 TCCGGA T^CCGGA MseI 1307 TTAA T^TAA NarI 10 GGCGCC GG^CGCC PflFI 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 957 CACGTG CAC^GTG PmlI 957 CACGTG CAC^GTG PvuII 922 CAGCTG CAG^CTG SacII 35 CCGCGG CCGC^GG SbfI 432 CCTGCAGG CCTGCA^GG SfoI 10 GGCGCC GGC^GCC Sse8387I 432 CCTGCAGG CCTGCA^GG SspBI 584 TGTACA T^GTACA StyI 965 CCAAGG C^C[AT][AT]GG Tru9I 1307 TTAA T^TAA Tth111I 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 10 GGCGCC G[GA]^CG[CT]C 39 GGCGTC AspHI 445 GAGCAC G[AT]GC[AT]^C 1012 GAGCAC BalI 120 TGGCCA TGG^CCA 1205 TGGCCA BanI 10 GGCGCC G^G[CT][GA]CC 754 GGCACC BbsI 412 GTCTTC GAAGAC(2/6) 451 GTCTTC BpuAI 412 GTCTTC GAAGAC(2/6) 451 GTCTTC BsaHI 10 GGCGCC G[GA]^CG[CT]C 39 GGCGTC BsiEI 63 CGGCCG CG[GA][CT]^CG 1445 CGGCCG BsiHKAI 445 GAGCAC G[AT]GC[AT]^C 1012 GAGCAC BssHII 68 GCGCGC G^CGCGC 70 GCGCGC BstAPI 314 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 549 GCAGAACGTGC DdeI 1081 CTGAG C^T[ACGT]AG 1364 CTGAG DsaI 35 CCGCGG C^C[GA][CT]GG 905 CCGTGG EagI 63 CGGCCG C^GGCCG 1445 CGGCCG EclXI 63 CGGCCG C^GGCCG 1445 CGGCCG Eco52I 63 CGGCCG C^GGCCG 1445 CGGCCG Hin1I 10 GGCGCC G[GA]^CG[CT]C 39 GGCGTC MluNI 120 TGGCCA TGG^CCA 1205 TGGCCA MscI 120 TGGCCA TGG^CCA 1205 TGGCCA NaeI 79 GCCGGC GCC^GGC 1060 GCCGGC NgoMIV 79 GCCGGC G^CCGGC 1060 GCCGGC NspI 494 ACATGT [GA]CATG^[CT] 684 ACATGC PpuMI 527 GGGACCT [GA]G^G[AT]CC[CT] 1334 AGGACCT PstI 433 CTGCAG CTGCA^G 544 CTGCAG SfcI 433 CTGCAG C^T[GA][CT]AG 544 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TseI TspRI TthHB8I XcmI XhoII XmaCI XmaI