(Circular) (Six-base) MAPSORT of: pFN21AE0956.seq Check: 9567 from: 1 to: 6163 pFN21AE0956 6163 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2556 2556 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 4226 5610 5878 1632 Size: 1715 879 1384 268 1917 Fragments arranged by size: 1917 1715 1384 879 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 2499 5062 6039 1203 Size: 1296 2563 977 1327 Fragments arranged by size: 2563 1327 1296 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 2839 4892 2839 Size: 2053 4110 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 2486 Size: 32 6131 AlwNI CAG_nnn'CTG Cuts at: 3906 5478 3906 Size: 1572 4591 ApaI G_GGCC'C Cuts at: 2140 2140 Size: 6163 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2133 2139 2146 3014 3225 3346 Size: 73 107 83 6 7 868 211 121 Cuts at: 3346 3488 1870 Size: 142 4545 Fragments arranged by size: 4545 868 211 142 121 107 83 73 7 6 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2759 3484 4939 618 Size: 325 217 627 228 744 725 1455 1842 Fragments arranged by size: 1842 1455 744 725 627 325 228 217 BanII G_rGCy'C Cuts at: 1391 2057 2140 2213 3482 1391 Size: 666 83 73 1269 4072 Fragments arranged by size: 4072 1269 666 83 73 BbeI G_GCGC'C Cuts at: 2019 2019 Size: 6163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 2449 928 Size: 971 511 39 4642 Fragments arranged by size: 4642 971 511 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2085 4774 136 Size: 107 122 71 631 605 413 2689 1525 Fragments arranged by size: 2689 1525 631 605 413 122 107 71 BglII A'GATC_T Cuts at: 2784 6158 2784 Size: 3374 2789 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2140 3120 4224 5380 1790 Size: 350 980 1104 1156 2573 Fragments arranged by size: 2573 1156 1104 980 350 BmgBI CAC'GTC Cuts at: 1318 2266 2455 3043 1318 Size: 948 189 588 4438 Fragments arranged by size: 4438 948 588 189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 3244 3894 Size: 416 53 83 399 24 1117 710 650 Cuts at: 3894 4852 442 Size: 958 1753 Fragments arranged by size: 1753 1117 958 710 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3372 3372 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2884 3013 3020 3179 3194 4823 1239 Size: 1211 434 129 7 159 15 1629 2579 Fragments arranged by size: 2579 1629 1211 434 159 129 15 7 Bpu10I CC'TnA_GC Cuts at: 3086 3086 Size: 6163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2964 3954 493 Size: 711 1760 990 2702 Fragments arranged by size: 2702 1760 990 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2045 3970 4352 Size: 53 83 186 936 483 29 1925 382 Cuts at: 4352 4987 275 Size: 635 1451 Fragments arranged by size: 1925 1451 936 635 483 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 2779 4596 4994 5268 5415 5826 2779 Size: 1817 398 274 147 411 3116 Fragments arranged by size: 3116 1817 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2372 2486 2516 2882 Size: 30 165 30 939 30 114 30 366 Cuts at: 2882 2912 3011 3041 1178 Size: 30 99 30 4300 Fragments arranged by size: 4300 939 366 165 114 99 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2507 2990 3176 3346 1186 Size: 837 484 483 186 170 4003 Fragments arranged by size: 4003 837 484 483 186 170 BseYI C'CCAG_C Cuts at: 5366 5862 5366 Size: 496 5667 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2582 2945 3065 3119 3173 1163 Size: 573 618 228 363 120 54 54 4153 Fragments arranged by size: 4153 618 573 363 228 120 54 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2071 3453 3613 4374 5402 1540 Size: 275 186 70 1382 160 761 1028 2301 Fragments arranged by size: 2301 1382 1028 761 275 186 160 70 BsiHKAI G_wGCw'C Cuts at: 2454 3021 4224 4309 5380 2454 Size: 567 1203 85 1071 3237 Fragments arranged by size: 3237 1203 1071 567 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2140 2213 2454 3021 3120 3482 Size: 399 267 83 73 241 567 99 362 Cuts at: 3482 4224 4309 5380 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 362 267 241 99 85 83 73 BspEI T'CCGG_A Cuts at: 2779 5826 2779 Size: 3047 3116 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 2544 2556 2631 3518 844 Size: 1682 18 12 75 887 3489 Fragments arranged by size: 3489 1682 887 75 18 12 BsrBI CCG'CTC Cuts at: 1394 1823 2160 2740 3459 4050 1394 Size: 429 337 580 719 591 3507 Fragments arranged by size: 3507 719 591 580 429 337 BsrDI GCAATG_nn' Cuts at: 65 1248 2712 4658 4832 65 Size: 1183 1464 1946 174 1396 Fragments arranged by size: 1946 1464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2242 3021 3065 4807 4994 1700 Size: 110 274 158 779 44 1742 187 2869 Fragments arranged by size: 2869 1742 779 274 187 158 110 44 BsrGI T'GTAC_A Cuts at: 96 2589 96 Size: 2493 3670 BssHII G'CGCG_C Cuts at: 1925 2073 2075 1925 Size: 148 2 6013 Fragments arranged by size: 6013 148 2 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 2325 2560 794 Size: 1531 235 4397 Fragments arranged by size: 4397 1531 235 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstXI CCAn_nnnn'nTGG Cuts at: 2977 2977 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2286 2433 2784 3493 3860 4245 4262 5714 Size: 314 147 351 709 367 385 17 1452 Cuts at: 5714 5800 5812 6158 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 351 346 314 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2910 513 Size: 552 975 870 3766 Fragments arranged by size: 3766 975 870 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2166 487 Size: 683 56 940 4484 Fragments arranged by size: 4484 940 683 56 BtsI GCAGTG_nn' Cuts at: 2393 3136 3679 4473 4493 2393 Size: 743 543 794 20 4063 Fragments arranged by size: 4063 794 743 543 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2125 2226 Size: 54 1425 50 275 18 238 57 101 Cuts at: 2226 2629 3063 3210 3450 3610 4499 6128 8 Size: 403 434 147 240 160 889 1629 43 Fragments arranged by size: 1629 1425 889 434 403 275 240 238 160 147 101 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2068 3450 3610 1537 Size: 275 256 1382 160 4090 Fragments arranged by size: 4090 1382 275 256 160 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 3089 3323 4093 1353 Size: 842 894 234 770 3423 Fragments arranged by size: 3423 894 842 770 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3406 3422 4750 5124 5270 387 Size: 1064 138 1817 16 1328 374 146 1280 Fragments arranged by size: 1817 1328 1280 1064 374 146 138 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2884 3013 3020 3179 3194 3347 Size: 393 818 434 129 7 159 15 153 Cuts at: 3347 4226 4823 5610 5878 1239 Size: 879 597 787 268 1524 Fragments arranged by size: 1524 879 818 787 597 434 393 268 159 153 129 15 7 EcoO109I rG'GnC_Cy Cuts at: 2533 2883 3183 3340 2533 Size: 350 300 157 5356 Fragments arranged by size: 5356 350 300 157 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2329 2329 Size: 6163 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2019 2040 3062 3208 5310 1678 Size: 341 21 1022 146 2102 2531 Fragments arranged by size: 2531 2102 1022 341 146 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2144 2176 2825 Size: 32 135 32 512 32 28 32 649 Cuts at: 2825 2857 2882 2914 4804 4836 4878 4910 1373 Size: 32 25 32 1890 32 42 32 2626 Fragments arranged by size: 2626 1890 649 512 135 42 32 32 32 32 32 32 32 32 28 25 HincII GTy'rAC Cuts at: 669 1657 1938 2803 3507 3753 669 Size: 988 281 865 704 246 3079 Fragments arranged by size: 3079 988 865 704 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KasI G'GCGC_C Cuts at: 2015 2015 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 5277 5461 1305 Size: 810 3162 184 2007 Fragments arranged by size: 3162 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2127 3212 10 Size: 54 1425 343 295 1085 2961 Fragments arranged by size: 2961 1425 1085 343 295 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 2510 2649 4121 4480 4639 518 Size: 648 1338 6 139 1472 359 159 2042 Fragments arranged by size: 2042 1472 1338 648 359 159 139 6 MspA1I CmG'CkG Cuts at: 1606 2042 2120 2929 3579 4256 5404 5649 1606 Size: 436 78 809 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 650 436 245 78 NaeI GCC'GGC Cuts at: 2086 3067 2086 Size: 981 5182 NarI GG'CG_CC Cuts at: 2016 2016 Size: 6163 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5611 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 2084 3065 2084 Size: 981 5182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 2503 2693 3521 5066 1132 Size: 97 1274 190 828 1545 2229 Fragments arranged by size: 2229 1545 1274 828 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6163 PciI A'CATG_T Cuts at: 2499 5062 2499 Size: 2563 3600 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6163 PfoI T'CCnGG_A Cuts at: 2421 2484 2673 3036 3171 2421 Size: 63 189 363 135 5413 Fragments arranged by size: 5413 363 189 135 63 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PmlI CAC'GTG Cuts at: 2964 2964 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2486 2518 4232 4264 5745 5777 2486 Size: 32 1714 32 1481 32 2872 Fragments arranged by size: 2872 1714 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2533 3340 2533 Size: 807 5356 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2136 2136 Size: 6163 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2459 2491 2459 Size: 32 6131 PstI C_TGCA'G Cuts at: 830 2442 2553 3515 830 Size: 1612 111 962 3478 Fragments arranged by size: 3478 1612 962 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 2929 2929 Size: 6163 SacII CC_GC'GG Cuts at: 2043 2043 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SbfI CC_TGCA'GG Cuts at: 2442 3515 2442 Size: 1073 5090 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2438 2549 3511 4646 5327 5518 826 Size: 219 1393 111 962 1135 681 191 1471 Fragments arranged by size: 1471 1393 1135 962 681 219 191 111 SfoI GGC'GCC Cuts at: 2017 2017 Size: 6163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 2052 2135 2141 3227 3490 1872 Size: 180 83 6 1086 263 4545 Fragments arranged by size: 4545 1086 263 180 83 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 4267 5168 5430 820 Size: 197 926 2324 901 262 1553 Fragments arranged by size: 2324 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2970 3602 513 Size: 552 1905 632 3074 Fragments arranged by size: 3074 1905 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 4190 4207 4360 4545 4990 325 Size: 1255 1137 1473 17 153 185 445 1498 Fragments arranged by size: 1498 1473 1255 1137 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 2589 4409 Size: 274 80 33 51 1443 393 219 1820 Cuts at: 4409 6121 96 Size: 1712 138 Fragments arranged by size: 1820 1712 1443 393 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 6123 6155 3011 Size: 32 3080 32 3019 Fragments arranged by size: 3080 3019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2405 1316 Size: 1089 5074 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 2908 2977 3154 2770 Size: 138 69 177 5779 Fragments arranged by size: 5779 177 138 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 2050 2133 2139 3225 3488 1870 Size: 180 83 6 1086 263 4545 Fragments arranged by size: 4545 1086 263 180 83 6 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI