(Linear) (Six-base) MAPSORT of: KIEE0956.seq Check: 643 from: 1 to: 1458 KIEE0956.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 551 1458 Size: 551 907 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1342 1458 Size: 1342 116 AflIII A'CryG_T Cuts at: 0 494 1458 Size: 494 964 AhdI GACnn_n'nnGTC Cuts at: 0 834 1458 Size: 834 624 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 481 513 1458 Size: 481 32 945 Fragments arranged by size: 945 481 32 ApaI G_GGCC'C Cuts at: 0 135 1458 Size: 135 1323 AvaI C'yCGr_G Cuts at: 0 45 128 134 141 1009 1220 1341 1458 Size: 45 83 6 7 868 211 121 117 Fragments arranged by size: 868 211 121 117 83 45 7 6 BanI G'GyrC_C Cuts at: 0 10 754 1458 Size: 10 744 704 Fragments arranged by size: 744 704 10 BanII G_rGCy'C Cuts at: 0 52 135 208 1458 Size: 52 83 73 1250 Fragments arranged by size: 1250 83 73 52 BbeI G_GCGC'C Cuts at: 0 14 1458 Size: 14 1444 BbsI GAAGACnn'nnnn_ Cuts at: 0 405 444 1458 Size: 405 39 1014 Fragments arranged by size: 1014 405 39 BglI GCCn_nnn'nGGC Cuts at: 0 80 1458 Size: 80 1378 BglII A'GATC_T Cuts at: 0 779 1458 Size: 779 679 Bme1580I G_kGCm'C Cuts at: 0 135 1115 1458 Size: 135 980 343 Fragments arranged by size: 980 343 135 BmgBI CAC'GTC Cuts at: 0 261 450 1038 1458 Size: 261 189 588 420 Fragments arranged by size: 588 420 261 189 BmrI ACTGGGnnnn_n' Cuts at: 0 529 1239 1458 Size: 529 710 219 Fragments arranged by size: 710 529 219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1367 1458 Size: 1367 91 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 879 1008 1015 1174 1189 1458 Size: 445 434 129 7 159 15 269 Fragments arranged by size: 445 434 269 159 129 15 7 Bpu10I CC'TnA_GC Cuts at: 0 1081 1458 Size: 1081 377 BsaAI yAC'GTr Cuts at: 0 959 1458 Size: 959 499 BsaHI Gr'CG_yC Cuts at: 0 11 40 1458 Size: 11 29 1418 Fragments arranged by size: 1418 29 11 BsaWI w'CCGG_w Cuts at: 0 774 1458 Size: 774 684 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 337 367 481 511 877 907 1006 1036 Size: 337 30 114 30 366 30 99 30 Cuts at: 1036 1458 Size: 422 Fragments arranged by size: 422 366 337 114 99 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 502 985 1171 1341 1458 Size: 18 484 483 186 170 117 Fragments arranged by size: 484 483 186 170 117 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 577 940 1060 1114 1168 1458 Size: 349 228 363 120 54 54 290 Fragments arranged by size: 363 349 290 228 120 54 54 BsiEI CG_ry'CG Cuts at: 0 66 1448 1458 Size: 66 1382 10 Fragments arranged by size: 1382 66 10 BsiHKAI G_wGCw'C Cuts at: 0 449 1016 1458 Size: 449 567 442 Fragments arranged by size: 567 449 442 Bsp1286I G_dGCh'C Cuts at: 0 52 135 208 449 1016 1115 1458 Size: 52 83 73 241 567 99 343 Fragments arranged by size: 567 343 241 99 83 73 52 BspEI T'CCGG_A Cuts at: 0 774 1458 Size: 774 684 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 521 539 551 626 1458 Size: 521 18 12 75 832 Fragments arranged by size: 832 521 75 18 12 BsrBI CCG'CTC Cuts at: 0 155 735 1454 1458 Size: 155 580 719 4 Fragments arranged by size: 719 580 155 4 BsrDI GCAATG_nn' Cuts at: 0 707 1458 Size: 707 751 BsrFI r'CCGG_y Cuts at: 0 79 237 1016 1060 1458 Size: 79 158 779 44 398 Fragments arranged by size: 779 398 158 79 44 BsrGI T'GTAC_A Cuts at: 0 584 1458 Size: 584 874 BssHII G'CGCG_C Cuts at: 0 68 70 1458 Size: 68 2 1388 Fragments arranged by size: 1388 68 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 320 555 1458 Size: 320 235 903 Fragments arranged by size: 903 320 235 BstXI CCAn_nnnn'nTGG Cuts at: 0 972 1458 Size: 972 486 BstYI r'GATC_y Cuts at: 0 281 428 779 1458 Size: 281 147 351 679 Fragments arranged by size: 679 351 281 147 BtgI C'CryG_G Cuts at: 0 35 905 1458 Size: 35 870 553 Fragments arranged by size: 870 553 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 161 1458 Size: 161 1297 BtsI GCAGTG_nn' Cuts at: 0 388 1131 1458 Size: 388 743 327 Fragments arranged by size: 743 388 327 EaeI y'GGCC_r Cuts at: 0 63 120 221 624 1058 1205 1445 1458 Size: 63 57 101 403 434 147 240 13 Fragments arranged by size: 434 403 240 147 101 63 57 13 EagI C'GGCC_G Cuts at: 0 63 1445 1458 Size: 63 1382 13 Fragments arranged by size: 1382 63 13 EarI CTCTTCn'nnn_ Cuts at: 0 190 1084 1318 1458 Size: 190 894 234 140 Fragments arranged by size: 894 234 190 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1401 1417 1458 Size: 1401 16 41 Fragments arranged by size: 1401 41 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 879 1008 1015 1174 1189 1342 1458 Size: 445 434 129 7 159 15 153 116 Fragments arranged by size: 445 434 159 153 129 116 15 7 EcoO109I rG'GnC_Cy Cuts at: 0 528 878 1178 1335 1458 Size: 528 350 300 157 123 Fragments arranged by size: 528 350 300 157 123 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 324 1458 Size: 324 1134 HaeII r_GCGC'y Cuts at: 0 14 35 1057 1203 1458 Size: 14 21 1022 146 255 Fragments arranged by size: 1022 255 146 21 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 139 171 820 852 877 909 Size: 79 32 28 32 649 32 25 32 Cuts at: 909 1458 Size: 549 Fragments arranged by size: 649 549 79 32 32 32 32 28 25 HincII GTy'rAC Cuts at: 0 798 1458 Size: 798 660 KasI G'GCGC_C Cuts at: 0 10 1458 Size: 10 1448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 110 1458 Size: 110 1348 MscI TGG'CCA Cuts at: 0 122 1207 1458 Size: 122 1085 251 Fragments arranged by size: 1085 251 122 MslI CAynn'nnrTG Cuts at: 0 499 505 644 1458 Size: 499 6 139 814 Fragments arranged by size: 814 499 139 6 MspA1I CmG'CkG Cuts at: 0 37 115 924 1458 Size: 37 78 809 534 Fragments arranged by size: 809 534 78 37 NaeI GCC'GGC Cuts at: 0 81 1062 1458 Size: 81 981 396 Fragments arranged by size: 981 396 81 NarI GG'CG_CC Cuts at: 0 11 1458 Size: 11 1447 NgoMIV G'CCGG_C Cuts at: 0 79 1060 1458 Size: 79 981 398 Fragments arranged by size: 981 398 79 NspI r_CATG'y Cuts at: 0 498 688 1458 Size: 498 190 770 Fragments arranged by size: 770 498 190 PciI A'CATG_T Cuts at: 0 494 1458 Size: 494 964 PfoI T'CCnGG_A Cuts at: 0 416 479 668 1031 1166 1458 Size: 416 63 189 363 135 292 Fragments arranged by size: 416 363 292 189 135 63 PmlI CAC'GTG Cuts at: 0 959 1458 Size: 959 499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 481 513 1458 Size: 481 32 945 Fragments arranged by size: 945 481 32 PpuMI rG'GwC_Cy Cuts at: 0 528 1335 1458 Size: 528 807 123 Fragments arranged by size: 807 528 123 PspOMI G'GGCC_C Cuts at: 0 131 1458 Size: 131 1327 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 454 486 1458 Size: 454 32 972 Fragments arranged by size: 972 454 32 PstI C_TGCA'G Cuts at: 0 437 548 1458 Size: 437 111 910 Fragments arranged by size: 910 437 111 PvuII CAG'CTG Cuts at: 0 924 1458 Size: 924 534 SacII CC_GC'GG Cuts at: 0 38 1458 Size: 38 1420 SbfI CC_TGCA'GG Cuts at: 0 437 1458 Size: 437 1021 SfcI C'TryA_G Cuts at: 0 433 544 1458 Size: 433 111 914 Fragments arranged by size: 914 433 111 SfoI GGC'GCC Cuts at: 0 12 1458 Size: 12 1446 SmaI CCC'GGG Cuts at: 0 47 130 136 1222 1458 Size: 47 83 6 1086 236 Fragments arranged by size: 1086 236 83 47 6 StyI C'CwwG_G Cuts at: 0 965 1458 Size: 965 493 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 712 1458 Size: 712 746 TatI w'GTAC_w Cuts at: 0 365 584 1458 Size: 365 219 874 Fragments arranged by size: 874 365 219 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1006 1038 1458 Size: 1006 32 420 Fragments arranged by size: 1006 420 32 Tth111I GACn'n_nGTC Cuts at: 0 400 1458 Size: 400 1058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 765 903 972 1149 1458 Size: 765 138 69 177 309 Fragments arranged by size: 765 309 177 138 69 XmaI C'CCGG_G Cuts at: 0 45 128 134 1220 1458 Size: 45 83 6 1086 238 Fragments arranged by size: 1086 238 83 45 6 Enzymes that do cut: AarI AcuI AflIII AhdI AloI ApaI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacII SbfI SfcI SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BpuEI BsaI BsaBI BseYI BsiWI BsmI BsmBI BspHI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI