################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspLU11I BsrGI BssHII BstBI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HindIII KpnI KspI MfeI MluI MunI NcoI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 124 GGCGCC G[GA]^CG[CT]C AlwNI 133 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 603 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 124 GGCGCC GGCGC^C BbvCI 1926 CCTCAGC CCTCAGC(-5/-2) BcgI 69 CGAGACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 521 CTAG C^TAG BlpI 303 GCTCAGC GC^T[ACGT]AGC Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC BsaBI 369 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 124 GGCGCC G[GA]^CG[CT]C BspDI 1042 ATCGAT AT^CGAT BspHI 1313 TCATGA T^CATGA BsrBI 43 CCGCTC CCGCTC(-3/-3) BsrDI 422 CATTGC GCAATG(2/0) BsrFI 1344 GCCGGC [GA]^CCGG[CT] BssSI 178 CTCGTG CACGAG(-5/-1) Bst1107I 1694 GTATAC GTA^TAC BstAPI 1233 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 311 GGTCACC G^GT[ACGT]ACC BstPI 311 GGTCACC G^GT[ACGT]ACC BstZ17I 1694 GTATAC GTA^TAC CelII 303 GCTCAGC GC^T[ACGT]AGC Cfr10I 1344 GCCGGC [GA]^CCGG[CT] ClaI 1042 ATCGAT AT^CGAT DrdI 1702 GACTTTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1986 GAAGAG CTCTTC(1/4) EcoNI 357 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 311 GGTCACC G^GT[ACGT]ACC EcoRV 491 GATATC GAT^ATC EcoT14I 750 CCAAGG C^C[AT][AT]GG HgaI 963 GACGC GACGC(5/10) Hin1I 124 GGCGCC G[GA]^CG[CT]C KasI 124 GGCGCC G^GCGCC Ksp632I 1986 GAAGAG CTCTTC(1/4) MaeI 521 CTAG C^TAG MamI 369 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1344 GCCGGC GCC^GGC NarI 124 GGCGCC GG^CGCC NdeI 2011 CATATG CA^TATG NgoMIV 1344 GCCGGC G^CCGGC PaeR7I 1174 CTCGAG C^TCGAG PpuMI 624 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 1313 TCATGA T^CATGA SalI 808 GTCGAC G^TCGAC SfoI 124 GGCGCC GGC^GCC SmaI 120 CCCGGG CCC^GGG StyI 750 CCAAGG C^C[AT][AT]GG XcmI 260 CCAACGGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1174 CTCGAG C^TCGAG XmaCI 120 CCCGGG C^CCGGG XmaI 120 CCCGGG C^CCGGG XmnI 603 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 808 GTCGAC GT^[AC][GT]AC 1694 GTATAC AccII 1008 CGCG CG^CG 1068 CGCG AflIII 1151 ACACGT A^C[GA][CT]GT 1208 ACGTGT Alw44I 976 GTGCAC G^TGCAC 1882 GTGCAC AocI 104 CCTCAGG CC^T[ACGT]AGG 317 CCTCAGG ApaI 322 GGGCCC GGGCC^C 985 GGGCCC ApaLI 976 GTGCAC G^TGCAC 1882 GTGCAC AvaI 120 CCCGGG C^[CT]CG[GA]G 1174 CTCGAG BalI 1055 TGGCCA TGG^CCA 1618 TGGCCA BglI 1019 GCCGGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1048 GCCATGATGGC BsoBI 120 CCCGGG C^[CT]CG[GA]G 1174 CTCGAG BstUI 1008 CGCG CG^CG 1068 CGCG BstXI 341 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 537 CCAACTGGATGG BstYI 368 AGATCC [GA]^GATC[CT] 379 AGATCC Bsu36I 104 CCTCAGG CC^T[ACGT]AGG 317 CCTCAGG DsaI 347 CCGTGG C^C[GA][CT]GG 1776 CCACGG EaeI 1055 TGGCCA [CT]^GGCC[GA] 1618 TGGCCA Eco81I 104 CCTCAGG CC^T[ACGT]AGG 317 CCTCAGG EcoRI 548 GAATTC G^AATTC 2038 GAATTC HaeII 124 GGCGCC [GA]GCGC^[CT] 1118 AGCGCC HpaI 643 GTTAAC GTT^AAC 696 GTTAAC MaeII 1153 ACGT A^CGT 1208 ACGT MflI 368 AGATCC [GA]^GATC[CT] 379 AGATCC MluNI 1055 TGGCCA TGG^CCA 1618 TGGCCA MscI 1055 TGGCCA TGG^CCA 1618 TGGCCA MseI 644 TTAA T^TAA 697 TTAA MvnI 1008 CGCG CG^CG 1068 CGCG PshAI 684 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1299 GACAAATGTC PstI 1231 CTGCAG CTGCA^G 1887 CTGCAG PvuII 883 CAGCTG CAG^CTG 897 CAGCTG ScaI 737 AGTACT AGT^ACT 1178 AGTACT Tru9I 644 TTAA T^TAA 697 TTAA Tsp45I 312 GTCAC ^GT[GC]AC 1711 GTCAC XhoII 368 AGATCC [GA]^GATC[CT] 379 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MroI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp509I TspRI TthHB8I