(Circular) (Six-base) MAPSORT of: pFN21AB9968.seq Check: 423 from: 1 to: 6805 pFN21AB9968 6805 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3243 3243 Size: 6805 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4615 278 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2814 3700 4148 1937 Size: 877 886 448 4594 Fragments arranged by size: 4594 886 877 448 Acc65I G'GTAC_C Cuts at: 4126 4126 Size: 6805 AclI AA'CG_TT Cuts at: 1563 4932 5305 1563 Size: 3369 373 3063 Fragments arranged by size: 3369 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2194 2966 3989 4868 6252 6520 1632 Size: 562 772 1023 879 1384 268 1917 Fragments arranged by size: 1917 1384 1023 879 772 562 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6608 AflIII A'CryG_T Cuts at: 1203 3156 3213 5704 6681 1203 Size: 1953 57 2491 977 1327 Fragments arranged by size: 2491 1953 1327 977 57 AgeI A'CCGG_T Cuts at: 5636 5636 Size: 6805 AhdI GACnn_n'nnGTC Cuts at: 5534 5534 Size: 6805 AleI CACnn'nnGTG Cuts at: 1166 3569 3894 1166 Size: 2403 325 4077 Fragments arranged by size: 4077 2403 325 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2700 3912 1184 Size: 1516 1212 4077 Fragments arranged by size: 4077 1516 1212 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3275 3307 3275 Size: 32 6773 AlwNI CAG_nnn'CTG Cuts at: 2143 4548 6120 2143 Size: 2405 1572 2828 Fragments arranged by size: 2828 2405 1572 ApaI G_GGCC'C Cuts at: 2331 2994 2331 Size: 663 6142 ApaLI G'TGCA_C Cuts at: 2981 3887 4862 6018 2981 Size: 906 975 1156 3768 Fragments arranged by size: 3768 1156 975 906 ApoI r'AATT_y Cuts at: 1493 2553 2612 3098 4043 4114 4340 4492 6601 Size: 1060 59 486 945 71 226 152 2109 Cuts at: 6601 1493 Size: 1697 Fragments arranged by size: 2109 1697 1060 945 486 226 152 71 59 AseI AT'TA_AT Cuts at: 160 1032 5359 160 Size: 872 4327 1606 Fragments arranged by size: 4327 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 AvaI C'yCGr_G Cuts at: 1870 1943 2125 3179 4130 1870 Size: 73 182 1054 951 4545 Fragments arranged by size: 4545 1054 951 182 73 BamHI G'GATC_C Cuts at: 4135 4502 4135 Size: 367 6438 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 3032 3251 4126 5581 Size: 325 217 627 342 903 219 875 1455 Cuts at: 5581 618 Size: 1842 Fragments arranged by size: 1842 1455 903 875 627 342 325 219 217 BanII G_rGCy'C Cuts at: 1391 2013 2062 2331 2994 3638 4124 1391 Size: 622 49 269 663 644 486 4072 Fragments arranged by size: 4072 663 644 622 486 269 49 BbeI G_GCGC'C Cuts at: 2133 2133 Size: 6805 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5834 BbvCI CC'TCA_GC Cuts at: 3932 3932 Size: 6805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2063 2097 4996 5030 1903 Size: 34 126 34 2899 34 3678 Fragments arranged by size: 3678 2899 126 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4697 5907 4697 Size: 1210 5595 BclI T'GATC_A Cuts at: 1548 1548 Size: 6805 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3030 3059 5416 Size: 107 122 71 631 605 1358 29 2357 Cuts at: 5416 136 Size: 1525 Fragments arranged by size: 2357 1525 1358 631 605 122 107 71 29 BglII A'GATC_T Cuts at: 6800 6800 Size: 6805 BlpI GC'TnA_GC Cuts at: 2309 4222 2309 Size: 1913 4892 Bme1580I G_kGCm'C Cuts at: 1790 2331 2985 2994 3035 3891 4866 6022 1790 Size: 541 654 9 41 856 975 1156 2573 Fragments arranged by size: 2573 1156 975 856 654 541 41 9 BmgBI CAC'GTC Cuts at: 1318 3214 1318 Size: 1896 4909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4536 5494 442 Size: 416 53 83 399 24 3119 958 1753 Fragments arranged by size: 3119 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6805 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2440 2440 Size: 6805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2680 3245 3302 3556 5465 1239 Size: 1145 296 565 57 254 1909 2579 Fragments arranged by size: 2579 1909 1145 565 296 254 57 Bpu10I CC'TnA_GC Cuts at: 3932 3932 Size: 6805 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2610 2939 4930 5795 6093 2610 Size: 329 1991 865 298 3322 Fragments arranged by size: 3322 1991 865 329 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2069 2887 4003 5468 882 Size: 617 570 818 1116 1465 2219 Fragments arranged by size: 2219 1465 1116 818 617 570 BsaAI yAC'GTr Cuts at: 493 1204 4596 493 Size: 711 3392 2702 Fragments arranged by size: 3392 2702 711 BsaBI GATnn'nnATC Cuts at: 2378 6799 2378 Size: 4421 2384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 4612 4994 5629 Size: 53 83 186 936 597 2482 382 635 Cuts at: 5629 275 Size: 1451 Fragments arranged by size: 2482 1451 936 635 597 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2119 2826 3312 5238 5636 5910 6057 6468 2119 Size: 707 486 1926 398 274 147 411 2456 Fragments arranged by size: 2456 1926 707 486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2666 2696 3203 Size: 30 165 30 597 30 636 30 507 Cuts at: 3203 3233 3275 3305 1178 Size: 30 42 30 4678 Fragments arranged by size: 4678 636 597 507 165 42 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2168 2171 2294 2381 2384 2429 3299 Size: 979 3 3 123 87 3 45 870 Cuts at: 3299 3302 3761 1186 Size: 3 459 4230 Fragments arranged by size: 4230 979 870 459 123 87 45 3 3 3 3 BseYI C'CCAG_C Cuts at: 2136 2987 3905 6008 6504 2136 Size: 851 918 2103 496 2437 Fragments arranged by size: 2437 2103 918 851 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2876 3608 3866 1163 Size: 573 1140 732 258 4102 Fragments arranged by size: 4102 1140 732 573 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4255 5016 6044 1540 Size: 275 186 2254 761 1028 2301 Fragments arranged by size: 2301 2254 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2361 2985 3891 4866 4951 6022 2190 Size: 171 624 906 975 85 1071 2973 Fragments arranged by size: 2973 1071 975 906 624 171 85 BsmI GAATG_Cn' Cuts at: 4316 4409 4316 Size: 93 6712 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2062 2190 2331 2361 2985 2994 Size: 399 223 49 128 141 30 624 9 Cuts at: 2994 3035 3638 3891 4124 4866 4951 6022 1391 Size: 41 603 253 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 624 603 399 253 233 223 141 128 85 49 41 30 9 BspEI T'CCGG_A Cuts at: 2119 2826 3312 6468 2119 Size: 707 486 3156 2456 Fragments arranged by size: 3156 2456 707 486 BspHI T'CATG_A Cuts at: 3318 4694 6424 3318 Size: 1376 1730 3699 Fragments arranged by size: 3699 1730 1376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 3243 3742 4160 844 Size: 1460 939 499 418 3489 Fragments arranged by size: 3489 1460 939 499 418 BsrBI CCG'CTC Cuts at: 1394 1823 2050 4692 1394 Size: 429 227 2642 3507 Fragments arranged by size: 3507 2642 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 2426 5300 5474 65 Size: 1183 1178 2874 174 1396 Fragments arranged by size: 2874 1396 1183 1178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3349 5449 5636 1700 Size: 110 1539 2100 187 2869 Fragments arranged by size: 2869 2100 1539 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6805 BssSI C'ACGA_G Cuts at: 2183 4865 5877 2183 Size: 2682 1012 3111 Fragments arranged by size: 3111 2682 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3244 794 Size: 2450 4355 BstBI TT'CG_AA Cuts at: 5609 5609 Size: 6805 BstEII G'GTnAC_C Cuts at: 1184 2316 1184 Size: 1132 5673 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2549 2353 Size: 196 6609 BstYI r'GATC_y Cuts at: 1972 2373 2384 4135 4502 4887 4904 6356 6442 Size: 401 11 1751 367 385 17 1452 86 Cuts at: 6442 6454 6800 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1751 1452 401 385 367 346 86 17 12 11 BstZ17I GTA'TAC Cuts at: 3701 3701 Size: 6805 Bsu36I CC'TnA_GG Cuts at: 2110 2323 2110 Size: 213 6592 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 3781 513 Size: 552 939 348 1429 3537 Fragments arranged by size: 3537 1429 939 552 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3478 3820 487 Size: 683 56 2252 342 3472 Fragments arranged by size: 3472 2252 683 342 56 BtsI GCAGTG_nn' Cuts at: 2079 3889 3901 4321 5115 5135 2079 Size: 1810 12 420 794 20 3749 Fragments arranged by size: 3749 1810 794 420 20 12 ClaI AT'CG_AT Cuts at: 1554 3048 1554 Size: 1494 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6770 DraI TTT'AAA Cuts at: 4109 4109 Size: 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3713 5812 809 Size: 507 2397 2099 1802 Fragments arranged by size: 2397 2099 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3060 3623 4252 Size: 54 1425 50 275 18 1230 563 629 Cuts at: 4252 5141 6770 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1230 889 629 563 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4252 1537 Size: 275 2440 4090 Fragments arranged by size: 4090 2440 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3986 4735 1353 Size: 2633 749 3423 Fragments arranged by size: 3423 2633 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2377 5392 5766 5912 387 Size: 1064 138 788 3015 374 146 1280 Fragments arranged by size: 3015 1280 1064 788 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2194 2384 2680 2966 3245 3302 3556 Size: 393 562 190 296 286 279 57 254 Cuts at: 3556 3989 4868 5465 6252 6520 1239 Size: 433 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 562 433 393 296 286 279 268 254 190 57 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 6805 EcoO109I rG'GnC_Cy Cuts at: 2114 2327 2630 2114 Size: 213 303 6289 Fragments arranged by size: 6289 303 213 EcoRI G'AATT_C Cuts at: 2553 4043 4114 4492 2553 Size: 1490 71 378 4866 Fragments arranged by size: 4866 1490 378 71 EcoRV GAT'ATC Cuts at: 2498 2498 Size: 6805 FspI TGC'GCA Cuts at: 5311 5311 Size: 6805 HaeII r_GCGC'y Cuts at: 1678 2133 3127 5952 1678 Size: 455 994 2825 2531 Fragments arranged by size: 2825 2531 994 455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2666 2698 3203 3235 5446 Size: 32 135 32 1094 32 505 32 2211 Cuts at: 5446 5478 5520 5552 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2211 1094 505 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2650 2703 2815 4149 4395 669 Size: 988 281 712 53 112 1334 246 3079 Fragments arranged by size: 3079 1334 988 712 281 246 112 53 HindIII A'AGCT_T Cuts at: 748 748 Size: 6805 HpaI GTT'AAC Cuts at: 2650 2703 4395 2650 Size: 53 1692 5060 Fragments arranged by size: 5060 1692 53 KasI G'GCGC_C Cuts at: 2129 2129 Size: 6805 KpnI G_GTAC'C Cuts at: 4130 4130 Size: 6805 MfeI C'AATT_G Cuts at: 4404 4404 Size: 6805 MluI A'CGCG_T Cuts at: 6681 6681 Size: 6805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5919 6103 1305 Size: 4614 184 2007 Fragments arranged by size: 4614 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3062 3625 10 Size: 54 1425 343 1230 563 3190 Fragments arranged by size: 3190 1425 1230 563 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2696 2783 2939 3569 3884 3894 Size: 648 1185 345 87 156 630 315 10 Cuts at: 3894 4763 5122 5281 518 Size: 869 359 159 2042 Fragments arranged by size: 2042 1185 869 648 630 359 345 315 159 156 87 10 MspA1I CmG'CkG Cuts at: 1606 2143 2167 2890 2904 4221 4898 6046 6291 Size: 537 24 723 14 1317 677 1148 245 Cuts at: 6291 1606 Size: 2120 Fragments arranged by size: 2120 1317 1148 723 677 537 245 24 14 NaeI GCC'GGC Cuts at: 3351 3351 Size: 6805 NarI GG'CG_CC Cuts at: 2130 2130 Size: 6805 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5314 Fragments arranged by size: 5314 939 552 NdeI CA'TA_TG Cuts at: 387 4017 387 Size: 3630 3175 NgoMIV G'CCGG_C Cuts at: 3349 3349 Size: 6805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6805 NotI GC'GGCC_GC Cuts at: 4252 4252 Size: 6805 NspI r_CATG'y Cuts at: 1132 1229 4163 5708 1132 Size: 97 2934 1545 2229 Fragments arranged by size: 2934 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3502 1348 Size: 2154 4651 PciI A'CATG_T Cuts at: 5704 5704 Size: 6805 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6805 PmeI GTTT'AAAC Cuts at: 4109 4109 Size: 6805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3307 4874 4906 6387 6419 3275 Size: 32 1567 32 1481 32 3661 Fragments arranged by size: 3661 1567 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2630 2630 Size: 6805 PshAI GACnn'nnGTC Cuts at: 1613 2693 3308 1613 Size: 1080 615 5110 Fragments arranged by size: 5110 1080 615 PsiI TTA'TAA Cuts at: 4375 4375 Size: 6805 PspOMI G'GGCC_C Cuts at: 2327 2990 2327 Size: 663 6142 PspXI vC'TCGA_Gb Cuts at: 3179 3179 Size: 6805 PstI C_TGCA'G Cuts at: 830 3240 3896 4157 830 Size: 2410 656 261 3478 Fragments arranged by size: 3478 2410 656 261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6805 PvuII CAG'CTG Cuts at: 2890 2904 2890 Size: 14 6791 SalI G'TCGA_C Cuts at: 1936 2813 4147 1936 Size: 877 1334 4594 Fragments arranged by size: 4594 1334 877 SbfI CC_TGCA'GG Cuts at: 4157 4157 Size: 6805 ScaI AGT'ACT Cuts at: 2744 3185 5053 6765 2744 Size: 441 1868 1712 2784 Fragments arranged by size: 2784 1868 1712 441 SfcI C'TryA_G Cuts at: 826 1045 3007 3236 3892 4153 5288 5969 6160 Size: 219 1962 229 656 261 1135 681 191 Cuts at: 6160 826 Size: 1471 Fragments arranged by size: 1962 1471 1135 681 656 261 229 219 191 SfoI GGC'GCC Cuts at: 2131 2131 Size: 6805 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6805 SmaI CCC'GGG Cuts at: 1872 2127 4132 1872 Size: 255 2005 4545 Fragments arranged by size: 4545 2005 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 2625 2954 3179 4909 5810 6072 Size: 197 926 682 329 225 1730 901 262 Cuts at: 6072 820 Size: 1553 Fragments arranged by size: 1730 1553 926 901 682 329 262 225 197 SnaBI TAC'GTA Cuts at: 493 4596 493 Size: 4103 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6805 SphI G_CATG'C Cuts at: 1132 4163 1132 Size: 3031 3774 SspI AAT'ATT Cuts at: 5 52 4729 5 Size: 47 4677 2081 Fragments arranged by size: 4677 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6805 StyI C'CwwG_G Cuts at: 513 1065 2004 2755 4244 513 Size: 552 939 751 1489 3074 Fragments arranged by size: 3074 1489 939 751 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3275 3515 4832 4849 5002 5187 5632 Size: 1255 1695 240 1317 17 153 185 445 Cuts at: 5632 325 Size: 1498 Fragments arranged by size: 1695 1498 1317 1255 445 240 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 3153 3183 Size: 274 80 33 51 1443 765 411 30 Cuts at: 3183 5051 6763 96 Size: 1868 1712 138 Fragments arranged by size: 1868 1712 1443 765 411 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 6765 6797 2000 Size: 32 4733 32 2008 Fragments arranged by size: 4733 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6805 XbaI T'CTAG_A Cuts at: 4141 4141 Size: 6805 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 6805 XhoI C'TCGA_G Cuts at: 1943 3179 1943 Size: 1236 5569 XmaI C'CCGG_G Cuts at: 1870 2125 4130 1870 Size: 255 2005 4545 Fragments arranged by size: 4545 2005 255 XmnI GAAnn'nnTTC Cuts at: 2612 4934 2612 Size: 2322 4483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4613 276 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI