(Linear) (Six-base) MAPSORT of: KIBB9968.seq Check: 7699 from: 1 to: 2100 KIBB9968.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1238 2100 Size: 1238 862 AccI GT'mk_AC Cuts at: 0 809 1695 2100 Size: 809 886 405 Fragments arranged by size: 886 809 405 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 189 961 1984 2100 Size: 189 772 1023 116 Fragments arranged by size: 1023 772 189 116 AflIII A'CryG_T Cuts at: 0 1151 1208 2100 Size: 1151 57 892 Fragments arranged by size: 1151 892 57 AleI CACnn'nnGTG Cuts at: 0 1564 1889 2100 Size: 1564 325 211 Fragments arranged by size: 1564 325 211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 695 1907 2100 Size: 695 1212 193 Fragments arranged by size: 1212 695 193 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1270 1302 2100 Size: 1270 32 798 Fragments arranged by size: 1270 798 32 AlwNI CAG_nnn'CTG Cuts at: 0 138 2100 Size: 138 1962 ApaI G_GGCC'C Cuts at: 0 326 989 2100 Size: 326 663 1111 Fragments arranged by size: 1111 663 326 ApaLI G'TGCA_C Cuts at: 0 976 1882 2100 Size: 976 906 218 Fragments arranged by size: 976 906 218 ApoI r'AATT_y Cuts at: 0 548 607 1093 2038 2100 Size: 548 59 486 945 62 Fragments arranged by size: 945 548 486 62 59 AvaI C'yCGr_G Cuts at: 0 120 1174 2100 Size: 120 1054 926 Fragments arranged by size: 1054 926 120 BanI G'GyrC_C Cuts at: 0 124 1027 1246 2100 Size: 124 903 219 854 Fragments arranged by size: 903 854 219 124 BanII G_rGCy'C Cuts at: 0 8 57 326 989 1633 2100 Size: 8 49 269 663 644 467 Fragments arranged by size: 663 644 467 269 49 8 BbeI G_GCGC'C Cuts at: 0 128 2100 Size: 128 1972 BbvCI CC'TCA_GC Cuts at: 0 1927 2100 Size: 1927 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 58 92 2100 Size: 58 34 2008 Fragments arranged by size: 2008 58 34 BglI GCCn_nnn'nGGC Cuts at: 0 1025 1054 2100 Size: 1025 29 1046 Fragments arranged by size: 1046 1025 29 BlpI GC'TnA_GC Cuts at: 0 304 2100 Size: 304 1796 Bme1580I G_kGCm'C Cuts at: 0 326 980 989 1030 1886 2100 Size: 326 654 9 41 856 214 Fragments arranged by size: 856 654 326 214 41 9 BmgBI CAC'GTC Cuts at: 0 1209 2100 Size: 1209 891 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 435 2100 Size: 435 1665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 675 1240 1297 1551 2100 Size: 379 296 565 57 254 549 Fragments arranged by size: 565 549 379 296 254 57 Bpu10I CC'TnA_GC Cuts at: 0 1927 2100 Size: 1927 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 605 934 2100 Size: 605 329 1166 Fragments arranged by size: 1166 605 329 BsaI GGTCTCn'nnnn_ Cuts at: 0 64 882 1998 2100 Size: 64 818 1116 102 Fragments arranged by size: 1116 818 102 64 BsaBI GATnn'nnATC Cuts at: 0 373 2100 Size: 373 1727 BsaHI Gr'CG_yC Cuts at: 0 125 2100 Size: 125 1975 BsaWI w'CCGG_w Cuts at: 0 114 821 1307 2100 Size: 114 707 486 793 Fragments arranged by size: 793 707 486 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 661 691 1198 1228 1270 1300 2100 Size: 25 636 30 507 30 42 30 800 Fragments arranged by size: 800 636 507 42 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 163 166 289 376 379 424 1294 Size: 160 3 3 123 87 3 45 870 Cuts at: 1294 1297 1756 2100 Size: 3 459 344 Fragments arranged by size: 870 459 344 160 123 87 45 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 131 982 1900 2100 Size: 131 851 918 200 Fragments arranged by size: 918 851 200 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 871 1603 1861 2100 Size: 871 732 258 239 Fragments arranged by size: 871 732 258 239 BsiHKAI G_wGCw'C Cuts at: 0 185 356 980 1886 2100 Size: 185 171 624 906 214 Fragments arranged by size: 906 624 214 185 171 Bsp1286I G_dGCh'C Cuts at: 0 8 57 185 326 356 980 989 1030 Size: 8 49 128 141 30 624 9 41 Cuts at: 1030 1633 1886 2100 Size: 603 253 214 Fragments arranged by size: 624 603 253 214 141 128 49 41 30 9 8 BspEI T'CCGG_A Cuts at: 0 114 821 1307 2100 Size: 114 707 486 793 Fragments arranged by size: 793 707 486 114 BspHI T'CATG_A Cuts at: 0 1313 2100 Size: 1313 787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 1238 1737 2100 Size: 299 939 499 363 Fragments arranged by size: 939 499 363 299 BsrBI CCG'CTC Cuts at: 0 45 2100 Size: 45 2055 BsrDI GCAATG_nn' Cuts at: 0 421 2100 Size: 421 1679 BsrFI r'CCGG_y Cuts at: 0 1344 2100 Size: 1344 756 BssSI C'ACGA_G Cuts at: 0 178 2100 Size: 178 1922 BstAPI GCAn_nnn'nTGC Cuts at: 0 1239 2100 Size: 1239 861 BstEII G'GTnAC_C Cuts at: 0 311 2100 Size: 311 1789 BstXI CCAn_nnnn'nTGG Cuts at: 0 348 544 2100 Size: 348 196 1556 Fragments arranged by size: 1556 348 196 BstYI r'GATC_y Cuts at: 0 368 379 2100 Size: 368 11 1721 Fragments arranged by size: 1721 368 11 BstZ17I GTA'TAC Cuts at: 0 1696 2100 Size: 1696 404 Bsu36I CC'TnA_GG Cuts at: 0 105 318 2100 Size: 105 213 1782 Fragments arranged by size: 1782 213 105 BtgI C'CryG_G Cuts at: 0 347 1776 2100 Size: 347 1429 324 Fragments arranged by size: 1429 347 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1473 1815 2100 Size: 1473 342 285 Fragments arranged by size: 1473 342 285 BtsI GCAGTG_nn' Cuts at: 0 74 1884 1896 2100 Size: 74 1810 12 204 Fragments arranged by size: 1810 204 74 12 ClaI AT'CG_AT Cuts at: 0 1043 2100 Size: 1043 1057 DrdI GACnn_nn'nnGTC Cuts at: 0 1708 2100 Size: 1708 392 EaeI y'GGCC_r Cuts at: 0 1055 1618 2100 Size: 1055 563 482 Fragments arranged by size: 1055 563 482 EarI CTCTTCn'nnn_ Cuts at: 0 1981 2100 Size: 1981 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 372 2100 Size: 372 1728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 189 379 675 961 1240 1297 1551 1984 Size: 189 190 296 286 279 57 254 433 Cuts at: 1984 2100 Size: 116 Fragments arranged by size: 433 296 286 279 254 190 189 116 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 361 2100 Size: 361 1739 EcoO109I rG'GnC_Cy Cuts at: 0 109 322 625 2100 Size: 109 213 303 1475 Fragments arranged by size: 1475 303 213 109 EcoRI G'AATT_C Cuts at: 0 548 2038 2100 Size: 548 1490 62 Fragments arranged by size: 1490 548 62 EcoRV GAT'ATC Cuts at: 0 493 2100 Size: 493 1607 HaeII r_GCGC'y Cuts at: 0 128 1122 2100 Size: 128 994 978 Fragments arranged by size: 994 978 128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 661 693 1198 1230 2100 Size: 661 32 505 32 870 Fragments arranged by size: 870 661 505 32 32 HincII GTy'rAC Cuts at: 0 645 698 810 2100 Size: 645 53 112 1290 Fragments arranged by size: 1290 645 112 53 HpaI GTT'AAC Cuts at: 0 645 698 2100 Size: 645 53 1402 Fragments arranged by size: 1402 645 53 KasI G'GCGC_C Cuts at: 0 124 2100 Size: 124 1976 MscI TGG'CCA Cuts at: 0 1057 1620 2100 Size: 1057 563 480 Fragments arranged by size: 1057 563 480 MslI CAynn'nnrTG Cuts at: 0 346 691 778 934 1564 1879 1889 2100 Size: 346 345 87 156 630 315 10 211 Fragments arranged by size: 630 346 345 315 211 156 87 10 MspA1I CmG'CkG Cuts at: 0 138 162 885 899 2100 Size: 138 24 723 14 1201 Fragments arranged by size: 1201 723 138 24 14 NaeI GCC'GGC Cuts at: 0 1346 2100 Size: 1346 754 NarI GG'CG_CC Cuts at: 0 125 2100 Size: 125 1975 NdeI CA'TA_TG Cuts at: 0 2012 2100 Size: 2012 88 NgoMIV G'CCGG_C Cuts at: 0 1344 2100 Size: 1344 756 PasI CC'CwG_GG Cuts at: 0 1497 2100 Size: 1497 603 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1270 1302 2100 Size: 1270 32 798 Fragments arranged by size: 1270 798 32 PpuMI rG'GwC_Cy Cuts at: 0 625 2100 Size: 625 1475 PshAI GACnn'nnGTC Cuts at: 0 688 1303 2100 Size: 688 615 797 Fragments arranged by size: 797 688 615 PspOMI G'GGCC_C Cuts at: 0 322 985 2100 Size: 322 663 1115 Fragments arranged by size: 1115 663 322 PspXI vC'TCGA_Gb Cuts at: 0 1174 2100 Size: 1174 926 PstI C_TGCA'G Cuts at: 0 1235 1891 2100 Size: 1235 656 209 Fragments arranged by size: 1235 656 209 PvuII CAG'CTG Cuts at: 0 885 899 2100 Size: 885 14 1201 Fragments arranged by size: 1201 885 14 SalI G'TCGA_C Cuts at: 0 808 2100 Size: 808 1292 ScaI AGT'ACT Cuts at: 0 739 1180 2100 Size: 739 441 920 Fragments arranged by size: 920 739 441 SfcI C'TryA_G Cuts at: 0 1002 1231 1887 2100 Size: 1002 229 656 213 Fragments arranged by size: 1002 656 229 213 SfoI GGC'GCC Cuts at: 0 126 2100 Size: 126 1974 SmaI CCC'GGG Cuts at: 0 122 2100 Size: 122 1978 SmlI C'TyrA_G Cuts at: 0 620 949 1174 2100 Size: 620 329 225 926 Fragments arranged by size: 926 620 329 225 StyI C'CwwG_G Cuts at: 0 750 2100 Size: 750 1350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1270 1510 2100 Size: 1270 240 590 Fragments arranged by size: 1270 590 240 TatI w'GTAC_w Cuts at: 0 737 1148 1178 2100 Size: 737 411 30 922 Fragments arranged by size: 922 737 411 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 2100 Size: 27 2073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 267 2100 Size: 267 1833 XhoI C'TCGA_G Cuts at: 0 1174 2100 Size: 1174 926 XmaI C'CCGG_G Cuts at: 0 120 2100 Size: 120 1980 XmnI GAAnn'nnTTC Cuts at: 0 607 2100 Size: 607 1493 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HpaI KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV PasI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SalI ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmrI BmtI BsaAI BsiEI BsiWI BsmI BsmBI BsrGI BssHII BstBI CspCI DraI DraIII EagI EcoICRI FalI FseI FspI FspAI HindIII KpnI MfeI MluI MmeI NcoI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PsiI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI ZraI