################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BpuAI BsaBI BsaI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI TfiI TspRI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AfaI 390 GTAC GT^AC AlwI 90 GGATC GGATC(4/5) AlwNI 283 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG BbvCI 291 GCTGAGG CCTCAGC(-5/-2) BglII 380 AGATCT A^GATCT BpmI 658 CTGGAG CTGGAG(16/14) BsaAI 391 TACGTG [CT]AC^GT[GA] BsaWI 736 TCCGGT [AT]^CCGG[AT] BsrBI 217 CCGCTC CCGCTC(-3/-3) BsrFI 904 GCCGGC [GA]^CCGG[CT] BssHII 350 GCGCGC G^CGCGC BstAPI 282 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 626 CCAGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 904 GCCGGC [GA]^CCGG[CT] HgaI 369 GACGC GACGC(5/10) KspI 896 CCGCGG CCGC^GG MaeII 392 ACGT A^CGT MseI 860 TTAA T^TAA MslI 330 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 904 GCCGGC GCC^GGC NcoI 335 CCATGG C^CATGG NgoMIV 904 GCCGGC G^CCGGC NlaIII 336 CATG CATG^ PflMI 387 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 165 GGGTCCC [GA]G^G[AT]CC[CT] PstI 688 CTGCAG CTGCA^G RsaI 390 GTAC GT^AC SacII 896 CCGCGG CCGC^GG SbfI 687 CCTGCAGG CCTGCA^GG Sse8387I 687 CCTGCAGG CCTGCA^GG StuI 692 AGGCCT AGG^CCT Tru9I 860 TTAA T^TAA Tsp45I 920 GTCAC ^GT[GC]AC Tsp509I 520 AATT ^AATT Van91I 387 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 387 CCAGTACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### ApaI 24 GGGCCC GGGCC^C 111 GGGCCC BsmFI 943 GGGAC GGGAC(10/14) 167 GTCCC BspMI 686 ACCTGC ACCTGC(4/8) 727 ACCTGC BsrI 524 ACTGG ACTGG(1/-1) 387 CCAGT BstYI 90 GGATCT [GA]^GATC[CT] 380 AGATCT DpnII 91 GATC ^GATC 381 GATC DsaI 335 CCATGG C^C[GA][CT]GG 896 CCGCGG EarI 579 GAAGAG CTCTTC(1/4) 585 GAAGAG EcoT14I 335 CCATGG C^C[AT][AT]GG 774 CCTTGG HphI 126 GGTGA GGTGA(8/7) 921 TCACC Ksp632I 579 GAAGAG CTCTTC(1/4) 585 GAAGAG MaeIII 401 GTAAC ^GT[ACGT]AC 920 GTCAC MboI 91 GATC ^GATC 381 GATC MflI 90 GGATCT [GA]^GATC[CT] 380 AGATCT MspA1I 355 CCGCTG C[AC]G^C[GT]G 896 CCGCGG NdeII 91 GATC ^GATC 381 GATC NotI 303 GCGGCCGC GC^GGCCGC 898 GCGGCCGC Sau3AI 91 GATC ^GATC 381 GATC SfcI 688 CTGCAG C^T[GA][CT]AG 930 CTACAG StyI 335 CCATGG C^C[AT][AT]GG 774 CCTTGG XhoII 90 GGATCT [GA]^GATC[CT] 380 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcyI AluI Aor51HI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BlpI BmyI Bpu1102I BsaHI BsaJI BseRI BsiEI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DraII EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MboII MluNI MnlI MscI MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI SmaI TseI XmaCI XmaI