Restriction map for
pFN21AE3126
(Circular) (Six-base) MAPSORT of: pFN21AE3126.seq Check: 3517 from: 1 to: 5659
pFN21AE3126 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2741 2741
Size: 5659
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 3722 5106 5374 1632
Size: 990 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1100 990 268
AfeI AGC'GCT
Cuts at: 2145 2661 2764 2145
Size: 516 103 5040
Fragments arranged by size:
5040 516 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2293 3402 4974 2293
Size: 1109 1572 2978
Fragments arranged by size:
2978 1572 1109
ApaI G_GGCC'C
Cuts at: 2033 2120 2033
Size: 87 5572
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2174 2200 2236 2623 2671 2720
Size: 73 134 97 26 36 387 48 49
Cuts at: 2720 2792 2984 1870
Size: 72 192 4545
Fragments arranged by size:
4545 387 192 134 97 73 72 49
48 36 26
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2252 2491 2653 2783
Size: 325 217 627 444 21 239 162 130
Cuts at: 2783 2980 4435 618
Size: 197 1455 1842
Fragments arranged by size:
1842 1455 627 444 325 239 217 197
162 130 21
BanII G_rGCy'C
Cuts at: 1391 2033 2120 2215 2670 2978 1391
Size: 642 87 95 455 308 4072
Fragments arranged by size:
4072 642 455 308 95 87
BbeI G_GCGC'C
Cuts at: 2235 2256 2657 2235
Size: 21 401 5237
Fragments arranged by size:
5237 401 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BbvCI CC'TCA_GC
Cuts at: 2297 2297
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2250 2280 2612
Size: 107 122 71 631 605 578 30 332
Cuts at: 2612 2805 4270 136
Size: 193 1465 1525
Fragments arranged by size:
1525 1465 631 605 578 332 193 122
107 71 30
BglII A'GATC_T
Cuts at: 2385 5654 2385
Size: 3269 2390
BlpI GC'TnA_GC
Cuts at: 2147 2270 2378 3076 2147
Size: 123 108 698 4730
Fragments arranged by size:
4730 698 123 108
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2120 2496 3720 4876 1790
Size: 243 87 376 1224 1156 2573
Fragments arranged by size:
2573 1224 1156 376 243 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2684 4319 1239
Size: 1445 1635 2579
Fragments arranged by size:
2579 1635 1445
Bpu10I CC'TnA_GC
Cuts at: 2297 2297
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2398 3450 493
Size: 711 1194 1052 2702
Fragments arranged by size:
2702 1194 1052 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2253 2654 3466
Size: 53 83 186 936 699 21 401 812
Cuts at: 3466 3848 4483 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 812 699 635 401 382 186
83 53 21
BsaWI w'CCGG_w
Cuts at: 2741 4092 4490 4764 4911 5322 2741
Size: 1351 398 274 147 411 3078
Fragments arranged by size:
3078 1351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2836 2866 1178
Size: 30 165 30 1433 30 3971
Fragments arranged by size:
3971 1433 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2216 2608 2921 1186
Size: 840 190 392 313 3924
Fragments arranged by size:
3924 840 392 313 190
BseYI C'CCAG_C
Cuts at: 4862 5358 4862
Size: 496 5163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2312 2616 2907 3109 3870 4898
Size: 275 186 311 304 291 202 761 1028
Cuts at: 4898 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 311 304 291 275 202
186
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2120 2215 2496 2670 2978 3720
Size: 399 243 87 95 281 174 308 742
Cuts at: 3720 3805 4876 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 281 243 174
95 87 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2700 2741 3014 844
Size: 1856 41 273 3489
Fragments arranged by size:
3489 1856 273 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 3546 1394
Size: 429 401 1322 3507
Fragments arranged by size:
3507 1322 429 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2909 4303 4490 1700
Size: 110 1099 1394 187 2869
Fragments arranged by size:
2869 1394 1099 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 2355 1925
Size: 430 5229
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 794
Size: 1499 4160
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2638 2638
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2095 2385 2989 3356 3741 3758 5210 5296
Size: 123 290 604 367 385 17 1452 86
Cuts at: 5296 5308 5654 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 604 385 367 346 290 123
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2340 2901 513
Size: 552 939 336 561 3271
Fragments arranged by size:
3271 939 561 552 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 487
Size: 683 56 1094 3826
Fragments arranged by size:
3826 1094 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2050 2281
Size: 54 1425 50 275 18 177 43 231
Cuts at: 2281 2309 2343 2613 2828 2890 2904 3106 3995
Size: 28 34 270 215 62 14 202 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 275 270 231 215 202
177 62 54 50 43 43 34 28
18 14
EagI C'GGCC_G
Cuts at: 1537 1812 2309 2613 2904 3106 1537
Size: 275 497 304 291 202 4090
Fragments arranged by size:
4090 497 304 291 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 2585 3589 1353
Size: 1226 6 1004 3423
Fragments arranged by size:
3423 1226 1004 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2444 2906 4246 4620 4766 387
Size: 1064 138 855 462 1340 374 146 1280
Fragments arranged by size:
1340 1280 1064 855 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 2684 3722 4319 5106 5374 1239
Size: 393 990 62 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 990 787 597 393 268 62
EcoO109I rG'GnC_Cy
Cuts at: 2030 2116 2117 2171 2797 2030
Size: 86 1 54 626 4892
Fragments arranged by size:
4892 626 86 54 1
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2147 2235 2256 2333 2657 2663 2766 4806
Size: 469 88 21 77 324 6 103 2040
Cuts at: 4806 1678
Size: 2531
Fragments arranged by size:
2531 2040 469 324 103 88 77 21
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KasI G'GCGC_C
Cuts at: 2231 2252 2653 2231
Size: 21 401 5237
Fragments arranged by size:
5237 401 21
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2345 2892 10
Size: 54 1425 343 177 336 547 2777
Fragments arranged by size:
2777 1425 547 343 336 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3617 3976 4135 518
Size: 648 1173 1278 359 159 2042
Fragments arranged by size:
2042 1278 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 2903 3075 3752 4900 5145 1606
Size: 756 541 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 677 541 245 172
NaeI GCC'GGC
Cuts at: 2911 2911
Size: 5659
NarI GG'CG_CC
Cuts at: 2232 2253 2654 2232
Size: 21 401 5237
Fragments arranged by size:
5237 401 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 2340 513
Size: 552 939 336 3832
Fragments arranged by size:
3832 939 552 336
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NgoMIV G'CCGG_C
Cuts at: 2909 2909
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 2309 2904 3106 2309
Size: 595 202 4862
Fragments arranged by size:
4862 595 202
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 1108
Size: 1290 4369
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2171
Size: 5659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2029 2116 2029
Size: 87 5572
PstI C_TGCA'G
Cuts at: 830 2697 3011 830
Size: 1867 314 3478
Fragments arranged by size:
3478 1867 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SacII CC_GC'GG
Cuts at: 2904 2904
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SanDI GG'GwC_CC
Cuts at: 2171 2171
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 2697 3011 2697
Size: 314 5345
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2693 2935 3007 4142 4823 5014 826
Size: 219 1648 242 72 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 681 242 219 191 72
SfoI GGC'GCC
Cuts at: 2233 2254 2655 2233
Size: 21 401 5237
Fragments arranged by size:
5237 401 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2079 2176 2238 2794 2986 1872
Size: 207 97 62 556 192 4545
Fragments arranged by size:
4545 556 207 192 97 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2699 1517
Size: 1182 4477
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 2779 3098 513
Size: 552 939 336 439 319 3074
Fragments arranged by size:
3074 939 552 439 336 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 2861 2966 3686 3703 3856 4041
Size: 1255 539 742 105 720 17 153 185
Cuts at: 4041 4486 325
Size: 445 1498
Fragments arranged by size:
1498 1255 742 720 539 445 185 153
105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2836 2868 5619 5651 2836
Size: 32 2751 32 2844
Fragments arranged by size:
2844 2751 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2399
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2077 2174 2236 2792 2984 1870
Size: 207 97 62 556 192 4545
Fragments arranged by size:
4545 556 207 192 97 62
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI