(Linear) (Six-base) MAPSORT of: KIEE3126.seq Check: 4932 from: 1 to: 954 KIEE3126.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 736 954 Size: 736 218 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 954 Size: 617 337 AfeI AGC'GCT Cuts at: 0 140 656 759 954 Size: 140 516 103 195 Fragments arranged by size: 516 195 140 103 AlwNI CAG_nnn'CTG Cuts at: 0 288 954 Size: 288 666 ApaI G_GGCC'C Cuts at: 0 28 115 954 Size: 28 87 839 Fragments arranged by size: 839 87 28 AvaI C'yCGr_G Cuts at: 0 72 169 195 231 618 666 715 787 Size: 72 97 26 36 387 48 49 72 Cuts at: 787 954 Size: 167 Fragments arranged by size: 387 167 97 72 72 49 48 36 26 BanI G'GyrC_C Cuts at: 0 226 247 486 648 778 954 Size: 226 21 239 162 130 176 Fragments arranged by size: 239 226 176 162 130 21 BanII G_rGCy'C Cuts at: 0 28 115 210 665 954 Size: 28 87 95 455 289 Fragments arranged by size: 455 289 95 87 28 BbeI G_GCGC'C Cuts at: 0 230 251 652 954 Size: 230 21 401 302 Fragments arranged by size: 401 302 230 21 BbvCI CC'TCA_GC Cuts at: 0 292 954 Size: 292 662 BglI GCCn_nnn'nGGC Cuts at: 0 245 275 607 800 954 Size: 245 30 332 193 154 Fragments arranged by size: 332 245 193 154 30 BglII A'GATC_T Cuts at: 0 380 954 Size: 380 574 BlpI GC'TnA_GC Cuts at: 0 142 265 373 954 Size: 142 123 108 581 Fragments arranged by size: 581 142 123 108 Bme1580I G_kGCm'C Cuts at: 0 28 115 491 954 Size: 28 87 376 463 Fragments arranged by size: 463 376 87 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 679 954 Size: 679 275 Bpu10I CC'TnA_GC Cuts at: 0 292 954 Size: 292 662 BsaAI yAC'GTr Cuts at: 0 393 954 Size: 393 561 BsaHI Gr'CG_yC Cuts at: 0 227 248 649 954 Size: 227 21 401 305 Fragments arranged by size: 401 305 227 21 BsaWI w'CCGG_w Cuts at: 0 736 954 Size: 736 218 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 831 861 954 Size: 831 30 93 Fragments arranged by size: 831 93 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 211 603 916 954 Size: 21 190 392 313 38 Fragments arranged by size: 392 313 190 38 21 BsiEI CG_ry'CG Cuts at: 0 307 611 902 954 Size: 307 304 291 52 Fragments arranged by size: 307 304 291 52 Bsp1286I G_dGCh'C Cuts at: 0 28 115 210 491 665 954 Size: 28 87 95 281 174 289 Fragments arranged by size: 289 281 174 95 87 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 695 736 954 Size: 695 41 218 Fragments arranged by size: 695 218 41 BsrBI CCG'CTC Cuts at: 0 219 954 Size: 219 735 BsrFI r'CCGG_y Cuts at: 0 904 954 Size: 904 50 BssHII G'CGCG_C Cuts at: 0 350 954 Size: 350 604 BstAPI GCAn_nnn'nTGC Cuts at: 0 288 954 Size: 288 666 BstXI CCAn_nnnn'nTGG Cuts at: 0 633 954 Size: 633 321 BstYI r'GATC_y Cuts at: 0 90 380 954 Size: 90 290 574 Fragments arranged by size: 574 290 90 BtgI C'CryG_G Cuts at: 0 335 896 954 Size: 335 561 58 Fragments arranged by size: 561 335 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 315 954 Size: 315 639 EaeI y'GGCC_r Cuts at: 0 2 45 276 304 338 608 823 885 Size: 2 43 231 28 34 270 215 62 Cuts at: 885 899 954 Size: 14 55 Fragments arranged by size: 270 231 215 62 55 43 34 28 14 2 EagI C'GGCC_G Cuts at: 0 304 608 899 954 Size: 304 304 291 55 Fragments arranged by size: 304 304 291 55 EarI CTCTTCn'nnn_ Cuts at: 0 574 580 954 Size: 574 6 374 Fragments arranged by size: 574 374 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 439 901 954 Size: 439 462 53 Fragments arranged by size: 462 439 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 679 954 Size: 617 62 275 Fragments arranged by size: 617 275 62 EcoO109I rG'GnC_Cy Cuts at: 0 25 111 112 166 792 954 Size: 25 86 1 54 626 162 Fragments arranged by size: 626 162 86 54 25 1 HaeII r_GCGC'y Cuts at: 0 142 230 251 328 652 658 761 954 Size: 142 88 21 77 324 6 103 193 Fragments arranged by size: 324 193 142 103 88 77 21 6 KasI G'GCGC_C Cuts at: 0 226 247 648 954 Size: 226 21 401 306 Fragments arranged by size: 401 306 226 21 MscI TGG'CCA Cuts at: 0 4 340 887 954 Size: 4 336 547 67 Fragments arranged by size: 547 336 67 4 MslI CAynn'nnrTG Cuts at: 0 334 954 Size: 334 620 MspA1I CmG'CkG Cuts at: 0 357 898 954 Size: 357 541 56 Fragments arranged by size: 541 357 56 NaeI GCC'GGC Cuts at: 0 906 954 Size: 906 48 NarI GG'CG_CC Cuts at: 0 227 248 649 954 Size: 227 21 401 305 Fragments arranged by size: 401 305 227 21 NcoI C'CATG_G Cuts at: 0 335 954 Size: 335 619 NgoMIV G'CCGG_C Cuts at: 0 904 954 Size: 904 50 NotI GC'GGCC_GC Cuts at: 0 304 899 954 Size: 304 595 55 Fragments arranged by size: 595 304 55 PflMI CCAn_nnn'nTGG Cuts at: 0 393 954 Size: 393 561 PpuMI rG'GwC_Cy Cuts at: 0 166 954 Size: 166 788 PspOMI G'GGCC_C Cuts at: 0 24 111 954 Size: 24 87 843 Fragments arranged by size: 843 87 24 PstI C_TGCA'G Cuts at: 0 692 954 Size: 692 262 SacII CC_GC'GG Cuts at: 0 899 954 Size: 899 55 SanDI GG'GwC_CC Cuts at: 0 166 954 Size: 166 788 SbfI CC_TGCA'GG Cuts at: 0 692 954 Size: 692 262 SfcI C'TryA_G Cuts at: 0 688 930 954 Size: 688 242 24 Fragments arranged by size: 688 242 24 SfoI GGC'GCC Cuts at: 0 228 249 650 954 Size: 228 21 401 304 Fragments arranged by size: 401 304 228 21 SmaI CCC'GGG Cuts at: 0 74 171 233 789 954 Size: 74 97 62 556 165 Fragments arranged by size: 556 165 97 74 62 StuI AGG'CCT Cuts at: 0 694 954 Size: 694 260 StyI C'CwwG_G Cuts at: 0 335 774 954 Size: 335 439 180 Fragments arranged by size: 439 335 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 114 856 954 Size: 114 742 98 Fragments arranged by size: 742 114 98 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 831 863 954 Size: 831 32 91 Fragments arranged by size: 831 91 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 394 954 Size: 394 560 XmaI C'CCGG_G Cuts at: 0 72 169 231 787 954 Size: 72 97 62 556 167 Fragments arranged by size: 556 167 97 72 62 Enzymes that do cut: AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PpuMI PspOMI PstI SacII SanDI SbfI SfcI SfoI SmaI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI