Restriction map for
pFN21AE6455
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI BbrPI BbvCI BciVI
BclI BfrI BglII BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MslI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1681 GACGTC GACGT^C
AccI 670 GTCGAC GT^[AC][GT]AC
AclI 1220 AACGTT AA^CGTT
AcsI 1611 GAATTC [GA]^AATT[CT]
ApoI 1611 GAATTC [GA]^AATT[CT]
AspHI 790 GAGCAC G[AT]GC[AT]^C
AviII 266 TGCGCA TGC^GCA
AvrII 762 CCTAGG C^CTAGG
BalI 855 TGGCCA TGG^CCA
BamHI 892 GGATCC G^GATCC
BbsI 1373 GAAGAC GAAGAC(2/6)
BlnI 762 CCTAGG C^CTAGG
BpuAI 1373 GAAGAC GAAGAC(2/6)
BsaI 232 GAGACC GGTCTC(1/5)
BsiHKAI 790 GAGCAC G[AT]GC[AT]^C
BspMI 269 GCAGGT ACCTGC(4/8)
BstXI 846 CCATGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 892 GGATCC [GA]^GATC[CT]
EagI 340 CGGCCG C^GGCCG
EarI 79 CTCTTC CTCTTC(1/4)
EclXI 340 CGGCCG C^GGCCG
Eco52I 340 CGGCCG C^GGCCG
EcoRI 1611 GAATTC G^AATTC
FspI 266 TGCGCA TGC^GCA
Ksp632I 79 CTCTTC CTCTTC(1/4)
MflI 892 GGATCC [GA]^GATC[CT]
MluNI 855 TGGCCA TGG^CCA
MscI 855 TGGCCA TGG^CCA
NspI 362 ACATGC [GA]CATG^[CT]
PflMI 858 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1220 AACGTT AA^CGTT
PvuII 1083 CAGCTG CAG^CTG
SalI 670 GTCGAC G^TCGAC
ScaI 1440 AGTACT AGT^ACT
SfcI 882 CTACAG C^T[GA][CT]AG
SmlI 832 CTCAAG C^T[CT][GA]AG
StuI 506 AGGCCT AGG^CCT
Van91I 858 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 892 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 1089 GTAC GT^AC
1441 GTAC
ApaI 208 GGGCCC GGGCC^C
402 GGGCCC
Asp700I 1324 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAAGTTTTTC
BcgI 26 CGACACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
284 CGAACGCGCTGC
BglI 247 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
655 GCCCCCGGGGC
BlpI 157 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
Bpu1102I 157 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
BsiEI 340 CGGCCG CG[GA][CT]^CG
668 CGGTCG
BsmAI 232 GAGAC GTCTC(1/5)
77 GTCTC
BsrDI 138 CATTGC GCAATG(2/0)
1568 GCAATG
CelII 157 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
EcoNI 252 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
828 CCTTCTCAAGG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
762 CCTAGG
EcoT22I 1538 ATGCAT ATGCA^T
1571 ATGCAT
HincII 670 GTCGAC GT[CT]^[GA]AC
1426 GTCAAC
HindII 670 GTCGAC GT[CT]^[GA]AC
1426 GTCAAC
MaeII 1221 ACGT A^CGT
1682 ACGT
NaeI 294 GCCGGC GCC^GGC
698 GCCGGC
NgoMIV 294 GCCGGC G^CCGGC
698 GCCGGC
NsiI 1538 ATGCAT ATGCA^T
1571 ATGCAT
PleI 75 GAGTC GAGTC(4/5)
1402 GAGTC
RsaI 1089 GTAC GT^AC
1441 GTAC
StyI 239 CCAAGG C^C[AT][AT]GG
762 CCTAGG
XcmI 299 CCAGCGGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1140 CCAAATCCTCACTGG
XmnI 1324 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PpuMI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI