Restriction map for
pFN21AE6455
(Circular) (Six-base) MAPSORT of: pFN21AE6455.seq Check: 5225 from: 1 to: 6412
pFN21AE6455 6412 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3690 4222 278
Size: 53 83 186 936 2154 532 2468
Fragments arranged by size:
2468 2154 936 532 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 3755 1937
Size: 739 1079 4594
Fragments arranged by size:
4594 1079 739
Acc65I G'GTAC_C
Cuts at: 3733 3733
Size: 6412
AclI AA'CG_TT
Cuts at: 1563 3226 4539 4912 1563
Size: 1663 1313 373 3063
Fragments arranged by size:
3063 1663 1313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2593 3495 4475 5859 6127 1632
Size: 961 902 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 961 902 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 5311 6288 1203
Size: 4108 977 1327
Fragments arranged by size:
4108 1327 977
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2766 1184
Size: 1582 4830
AlwNI CAG_nnn'CTG
Cuts at: 4155 5727 4155
Size: 1572 4840
ApaI G_GGCC'C
Cuts at: 2217 2411 2217
Size: 194 6218
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 3616 3721 3947 4099 6208 1493
Size: 2123 105 226 152 2109 1697
Fragments arranged by size:
2123 2109 1697 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 4966 160
Size: 872 3934 1606
Fragments arranged by size:
3934 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2222 2404 2481 2663 3737 1870
Size: 73 132 147 182 77 182 1074 4545
Fragments arranged by size:
4545 1074 182 182 147 132 77 73
AvrII C'CTAG_G
Cuts at: 2767 2767
Size: 6412
BamHI G'GATC_C
Cuts at: 2897 3742 4109 2897
Size: 845 367 5200
Fragments arranged by size:
5200 845 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2446 2706 2763 3733
Size: 325 217 627 462 197 260 57 970
Cuts at: 3733 5188 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 970 627 462 325 260 217
197 57
BanII G_rGCy'C
Cuts at: 1391 2217 2411 2425 3731 1391
Size: 826 194 14 1306 4072
Fragments arranged by size:
4072 1306 826 194 14
BbeI G_GCGC'C
Cuts at: 2253 2450 2767 2253
Size: 197 317 5898
Fragments arranged by size:
5898 317 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3385 928
Size: 971 1486 3955
Fragments arranged by size:
3955 1486 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2020 2021 2054 2278 2312 4603
Size: 34 50 33 1 33 224 34 2291
Cuts at: 4603 4637 1903
Size: 34 3678
Fragments arranged by size:
3678 2291 224 50 34 34 34 33
33 1
BciVI GTATCCnnnnn_n'
Cuts at: 4304 5514 4304
Size: 1210 5202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BfrBI ATG'CAT
Cuts at: 3545 3578 3545
Size: 33 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 2666 5023
Size: 107 122 71 631 605 586 408 2357
Cuts at: 5023 136
Size: 1525
Fragments arranged by size:
2357 1525 631 605 586 408 122 107
71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 2163 2531 3829 2163
Size: 368 1298 4746
Fragments arranged by size:
4746 1298 368
Bme1580I G_kGCm'C
Cuts at: 1790 2217 2411 4473 5629 1790
Size: 427 194 2062 1156 2573
Fragments arranged by size:
2573 2062 1156 427 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2211 4143 5101
Size: 416 53 83 399 24 794 1932 958
Cuts at: 5101 442
Size: 1753
Fragments arranged by size:
1932 1753 958 794 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2413 3274 5072 1239
Size: 893 281 861 1798 2579
Fragments arranged by size:
2579 1798 893 861 281
Bpu10I CC'TnA_GC
Cuts at: 2558 2558
Size: 6412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 4537 5402 5700 2822
Size: 1715 865 298 3534
Fragments arranged by size:
3534 1715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 5075 882
Size: 617 732 2844 2219
Fragments arranged by size:
2844 2219 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 4203 493
Size: 711 2999 2702
Fragments arranged by size:
2999 2702 711
BsaBI GATnn'nnATC
Cuts at: 6406 6406
Size: 6412
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2447 2630 2764
Size: 53 83 186 936 717 197 183 134
Cuts at: 2764 3687 4219 4601 5236 275
Size: 923 532 382 635 1451
Fragments arranged by size:
1451 936 923 717 635 532 382 197
186 183 134 83 53
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6187
Fragments arranged by size:
6187 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6412
BseYI C'CCAG_C
Cuts at: 2070 2545 2686 5615 6111 2070
Size: 475 141 2929 496 2371
Fragments arranged by size:
2929 2371 496 475 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5839
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2348 2676 3862 4623 5651 1540
Size: 275 186 347 328 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 347 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 2799 4473 4558 5629 2799
Size: 1674 85 1071 3582
Fragments arranged by size:
3582 1674 1071 85
BsmI GAATG_Cn'
Cuts at: 3923 4016 3923
Size: 93 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2411 2425 2799 3731 4473 4558
Size: 399 427 194 14 374 932 742 85
Cuts at: 4558 5629 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 932 742 427 399 374 194
85 14
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 3767 844
Size: 1421 1502 3489
Fragments arranged by size:
3489 1502 1421
BsrBI CCG'CTC
Cuts at: 1394 1823 2103 2427 2739 4299 1394
Size: 429 280 324 312 1560 3507
Fragments arranged by size:
3507 1560 429 324 312 280
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 3580 4907 5081 65
Size: 1183 894 1438 1327 174 1396
Fragments arranged by size:
1438 1396 1327 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2299 2343 2703 5056 5243 1700
Size: 110 489 44 360 2353 187 2869
Fragments arranged by size:
2869 2353 489 360 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 4472 5484 4472
Size: 1012 5400
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6412
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6412
BstXI CCAn_nnnn'nTGG
Cuts at: 2858 2858
Size: 6412
BstYI r'GATC_y
Cuts at: 1972 2897 3742 4109 4494 4511 5963 6049 6061
Size: 925 845 367 385 17 1452 86 12
Cuts at: 6061 6407 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 925 845 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2441 2574 2670 2720 513
Size: 552 1376 133 96 50 4205
Fragments arranged by size:
4205 1376 552 133 96 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5673
Fragments arranged by size:
5673 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2123 3928 4722 4742 2033
Size: 90 1805 794 20 3703
Fragments arranged by size:
3703 1805 794 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2301 2322
Size: 54 1425 50 275 18 429 42 21
Cuts at: 2322 2334 2345 2577 2655 2844 2860 3859 4748
Size: 12 11 232 78 189 16 999 889
Cuts at: 4748 6377 8
Size: 1629 43
Fragments arranged by size:
1629 1425 999 889 429 275 232 189
78 54 50 43 42 21 18 16
12 11
EagI C'GGCC_G
Cuts at: 1537 1812 2345 3859 1537
Size: 275 533 1514 4090
Fragments arranged by size:
4090 1514 533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 4342 1353
Size: 737 2252 3423
Fragments arranged by size:
3423 2252 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2377 2514 4999 5373 5519 387
Size: 1064 138 788 137 2485 374 146 1280
Fragments arranged by size:
2485 1280 1064 788 374 146 138 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2413 2593 3274 3495 4475 5072
Size: 393 500 281 180 681 221 980 597
Cuts at: 5072 5859 6127 1239
Size: 787 268 1524
Fragments arranged by size:
1524 980 787 681 597 500 393 281
268 221 180
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2837 2261
Size: 576 5836
EcoO109I rG'GnC_Cy
Cuts at: 2213 2359 2554 2650 3136 3280 2213
Size: 146 195 96 486 144 5345
Fragments arranged by size:
5345 486 195 146 144 96
EcoRI G'AATT_C
Cuts at: 3616 3721 4099 3616
Size: 105 378 5929
Fragments arranged by size:
5929 378 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 6412
FspI TGC'GCA
Cuts at: 2273 4918 2273
Size: 2645 3767
HaeII r_GCGC'y
Cuts at: 1678 2253 2437 2450 2603 2767 5559 1678
Size: 575 184 13 153 164 2792 2531
Fragments arranged by size:
2792 2531 575 184 164 153 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5053 5085 5127 5159 1373
Size: 32 135 32 3481 32 42 32 2626
Fragments arranged by size:
3481 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3433 3756 4002 669
Size: 988 281 739 756 323 246 3079
Fragments arranged by size:
3079 988 756 739 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KasI G'GCGC_C
Cuts at: 2249 2446 2763 2249
Size: 197 317 5898
Fragments arranged by size:
5898 317 197
KpnI G_GTAC'C
Cuts at: 3737 3737
Size: 6412
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2054 2925 5526 5710 1305
Size: 749 871 2601 184 2007
Fragments arranged by size:
2601 2007 871 749 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2862 10
Size: 54 1425 343 1030 3560
Fragments arranged by size:
3560 1425 1030 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4370 4729 4888 518
Size: 648 3204 359 159 2042
Fragments arranged by size:
3204 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2284 2307 2443 2452 2587 2635 2672
Size: 561 117 23 136 9 135 48 37
Cuts at: 2672 2722 3090 3828 4505 5653 5898 1606
Size: 50 368 738 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 561 368 245 136
135 117 50 48 37 23 9
NaeI GCC'GGC
Cuts at: 2301 2705 2301
Size: 404 6008
NarI GG'CG_CC
Cuts at: 2250 2447 2764 2250
Size: 197 317 5898
Fragments arranged by size:
5898 317 197
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5860
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 2299 2703 2299
Size: 404 6008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NsiI A_TGCA'T
Cuts at: 3547 3580 3547
Size: 33 6379
NspI r_CATG'y
Cuts at: 1132 1229 2371 3770 5315 1132
Size: 97 1142 1399 1545 2229
Fragments arranged by size:
2229 1545 1399 1142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 1108
Size: 1761 4651
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2650 3136 2359
Size: 291 486 5635
Fragments arranged by size:
5635 486 291
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PspOMI G'GGCC_C
Cuts at: 2213 2407 2213
Size: 194 6218
PstI C_TGCA'G
Cuts at: 830 3764 830
Size: 2934 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 3090 3090
Size: 6412
SacII CC_GC'GG
Cuts at: 2444 2673 2723 2444
Size: 229 50 6133
Fragments arranged by size:
6133 229 50
SalI G'TCGA_C
Cuts at: 1936 2675 3754 1936
Size: 739 1079 4594
Fragments arranged by size:
4594 1079 739
SanDI GG'GwC_CC
Cuts at: 2359 3136 2359
Size: 777 5635
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 3447 4660 6372 3447
Size: 1213 1712 3487
Fragments arranged by size:
3487 1712 1213
SfcI C'TryA_G
Cuts at: 826 1045 2887 3760 4895 5576 5767 826
Size: 219 1842 873 1135 681 191 1471
Fragments arranged by size:
1842 1471 1135 873 681 219 191
SfoI GGC'GCC
Cuts at: 2251 2448 2765 2251
Size: 197 317 5898
Fragments arranged by size:
5898 317 197
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 2406 2483 2665 3739 1872
Size: 534 77 182 1074 4545
Fragments arranged by size:
4545 1074 534 182 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 4516 5417 5679 820
Size: 197 926 894 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 894 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 3770 1132
Size: 2638 3774
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2513 1517
Size: 996 5416
StyI C'CwwG_G
Cuts at: 513 1065 2244 2767 3851 513
Size: 552 1179 523 1084 3074
Fragments arranged by size:
3074 1179 1084 552 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3390 4439 4456 4609 4794 5239
Size: 1255 504 1306 1049 17 153 185 445
Cuts at: 5239 325
Size: 1498
Fragments arranged by size:
1498 1306 1255 1049 504 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3445 4658 6370
Size: 274 80 33 51 1443 1468 1213 1712
Cuts at: 6370 96
Size: 138
Fragments arranged by size:
1712 1468 1443 1213 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6372 6404 6372
Size: 32 6380
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 3152 3706 2311
Size: 841 554 5017
Fragments arranged by size:
5017 841 554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 2404 2481 2663 3737 1870
Size: 534 77 182 1074 4545
Fragments arranged by size:
4545 1074 534 182 77
XmnI GAAnn'nnTTC
Cuts at: 3333 3480 4541 3333
Size: 147 1061 5204
Fragments arranged by size:
5204 1061 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3688 4220 276
Size: 53 83 186 936 2154 532 2468
Fragments arranged by size:
2468 2154 936 532 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI PacI PmlI PspXI PsrI RsrII SacI SapI
SexAI SfiI SrfI SwaI