################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AgeI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsgI BsiWI BsmI BsoBI BspDI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MfeI MluI MspA1I MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 1064 TCCGGA T^CCGGA AhdI 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1885 GTGCAC G^TGCAC AocI 365 CCTCAGG CC^T[ACGT]AGG ApaLI 1885 GTGCAC G^TGCAC AspEI 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 159 GGATCC G^GATCC BanII 599 GAGCTC G[GA]GC[CT]^C BbsI 1513 GAAGAC GAAGAC(2/6) BclI 516 TGATCA T^GATCA BcnI 1644 CCGGG CC^[GC]GG BpmI 1550 CTGGAG CTGGAG(16/14) BpuAI 1513 GAAGAC GAAGAC(2/6) BsaAI 39 TACGTA [CT]AC^GT[GA] BsaBI 154 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1064 TCCGGA T^CCGGA BsiEI 2071 CGGCCG CG[GA][CT]^CG BsiYI 326 CCCAACGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 326 CCCAACGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 1214 GAGACG CGTCTC(1/5) BsmFI 665 GGGAC GGGAC(10/14) BspEI 1064 TCCGGA T^CCGGA BspLU11I 272 ACATGT A^CATGT BstAPI 972 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 365 CCTCAGG CC^T[ACGT]AGG DsaI 1772 CCATGG C^C[GA][CT]GG EagI 2071 CGGCCG C^GGCCG Eam1105I 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2071 CGGCCG C^GGCCG Eco52I 2071 CGGCCG C^GGCCG Eco81I 365 CCTCAGG CC^T[ACGT]AGG FauI 663 GCGGG CCCGC(4/6) FbaI 516 TGATCA T^GATCA HgaI 1569 GACGC GACGC(5/10) HindIII 1557 AAGCTT A^AGCTT MamI 154 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1064 TCCGGA T^CCGGA MslI 2028 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1644 CCGGG CC^[GC]GG NcoI 1772 CCATGG C^CATGG NdeI 476 CATATG CA^TATG NheI 981 GCTAGC G^CTAGC NspI 272 ACATGT [GA]CATG^[CT] PstI 1316 CTGCAG CTGCA^G SacI 599 GAGCTC GAGCT^C SalI 1289 GTCGAC G^TCGAC SapI 792 GCTCTTC GCTCTTC(1/4) SfaNI 1361 GCATC GCATC(5/9) SnaBI 39 TACGTA TAC^GTA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 739 GTAGAC GT^[AC][GT]AC 1289 GTCGAC AflIII 272 ACATGT A^C[GA][CT]GT 1276 ACACGT AlwNI 368 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 557 CAGAGACTG AspHI 599 GAGCTC G[AT]GC[AT]^C 1885 GTGCAC AvaII 335 GGACC G^G[AT]CC 1259 GGTCC BalI 52 TGGCCA TGG^CCA 292 TGGCCA BsaWI 1064 TCCGGA [AT]^CCGG[AT] 1310 ACCGGA BseRI 356 CTCCTC GAGGAG(10/8) 863 GAGGAG BsiHKAI 599 GAGCTC G[AT]GC[AT]^C 1885 GTGCAC DraI 124 TTTAAA TTT^AAA 745 TTTAAA HincII 1289 GTCGAC GT[CT]^[GA]AC 1858 GTTGAC HindII 1289 GTCGAC GT[CT]^[GA]AC 1858 GTTGAC MluNI 52 TGGCCA TGG^CCA 292 TGGCCA MscI 52 TGGCCA TGG^CCA 292 TGGCCA SfcI 347 CTACAG C^T[GA][CT]AG 1316 CTGCAG SmlI 378 CTCAAG C^T[CT][GA]AG 1491 CTTGAG TaqI 603 TCGA T^CGA 1290 TCGA TthHB8I 603 TCGA T^CGA 1290 TCGA XcmI 87 CCAAAGACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1246 CCAATTATTAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BfaI BmyI BsaJI BsmAI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII