################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Alw44I AocI ApaLI AscI Asp718I AspI AviII AvrII BbvCI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SapI SfiI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 285 TCCGGA T^CCGGA AcsI 1019 GAATTT [GA]^AATT[CT] AfaI 1197 GTAC GT^AC AhdI 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1045 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1019 GAATTT [GA]^AATT[CT] AseI 520 ATTAAT AT^TAAT AsnI 520 ATTAAT AT^TAAT Asp700I 1782 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2168 TGGCCA TGG^CCA BamHI 2174 GGATCC G^GATCC BbsI 1602 GAAGAC GAAGAC(2/6) BcgI 1914 GCAGGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1572 GTATCC GTATCC(6/5) BclI 363 TGATCA T^GATCA BpuAI 1602 GAAGAC GAAGAC(2/6) BsaWI 285 TCCGGA [AT]^CCGG[AT] BseAI 285 TCCGGA T^CCGGA BsgI 1177 GTGCAG GTGCAG(16/14) BsmI 1209 GAATGC GAATGC(1/-1) BspEI 285 TCCGGA T^CCGGA BspHI 1682 TCATGA T^CATGA BsrBI 208 GAGCGG CCGCTC(-3/-3) BstBI 1813 TTCGAA TT^CGAA BstXI 1823 CCATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1374 TTTAAA TTT^AAA Eam1105I 1406 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2250 GAAGAG CTCTTC(1/4) EcoT14I 794 CCATGG C^C[AT][AT]GG FbaI 363 TGATCA T^GATCA Ksp632I 2250 GAAGAG CTCTTC(1/4) MluNI 2168 TGGCCA TGG^CCA MroI 285 TCCGGA T^CCGGA MscI 2168 TGGCCA TGG^CCA NcoI 794 CCATGG C^CATGG NspV 1813 TTCGAA TT^CGAA PleI 500 GACTC GAGTC(4/5) PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT] PshBI 520 ATTAAT AT^TAAT PstI 1546 CTGCAG CTGCA^G PvuII 1861 CAGCTG CAG^CTG RcaI 1682 TCATGA T^CATGA RsaI 1197 GTAC GT^AC SbfI 1545 CCTGCAGG CCTGCA^GG ScaI 1196 AGTACT AGT^ACT SfcI 1546 CTGCAG C^T[GA][CT]AG SfuI 1813 TTCGAA TT^CGAA Sse8387I 1545 CCTGCAGG CCTGCA^GG StuI 1286 AGGCCT AGG^CCT StyI 794 CCATGG C^C[AT][AT]GG XmnI 1782 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 701 ACACGT A^C[GA][CT]GT 1637 ACGTGT Aor51HI 27 AGCGCT AGC^GCT 470 AGCGCT ApaI 190 GGGCCC GGGCC^C 1130 GGGCCC BbrPI 702 CACGTG CAC^GTG 1636 CACGTG BglII 116 AGATCT A^GATCT 1070 AGATCT BsaI 163 GAGACC GGTCTC(1/5) 1489 GAGACC BsmBI 1237 GAGACG CGTCTC(1/5) 2253 GAGACG BspMI 1544 ACCTGC ACCTGC(4/8) 1914 GCAGGT BsrDI 689 GCAATG GCAATG(2/0) 1139 GCAATG BssHII 142 GCGCGC G^CGCGC 1213 GCGCGC DraIII 1225 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2133 CACAAGGTG EaeI 59 TGGCCG [CT]^GGCC[GA] 2168 TGGCCA Eco47III 27 AGCGCT AGC^GCT 470 AGCGCT EcoNI 295 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1227 CCTGGTGGAGG PaeR7I 88 CTCGAG C^TCGAG 160 CTCGAG PflMI 409 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCACCTGGTGG PmaCI 702 CACGTG CAC^GTG 1636 CACGTG PmlI 702 CACGTG CAC^GTG 1636 CACGTG SacI 157 GAGCTC GAGCT^C 339 GAGCTC SmaI 9 CCCGGG CCC^GGG 193 CCCGGG Van91I 409 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCACCTGGTGG XcmI 929 CCACTGATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2099 CCAAGAACTGTATGG XhoI 88 CTCGAG C^TCGAG 160 CTCGAG XmaCI 9 CCCGGG C^CCGGG 193 CCCGGG XmaI 9 CCCGGG C^CCGGG 193 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SexAI SfaNI SfoI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII