(Circular) (Six-base) MAPSORT of: pFN21AE6762.seq Check: 9494 from: 1 to: 6973 pFN21AE6762 6973 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 1937 Size: 2379 4594 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3077 4259 5036 6420 6688 1632 Size: 1445 1182 777 1384 268 1917 Fragments arranged by size: 1917 1445 1384 1182 777 268 AfeI AGC'GCT Cuts at: 2034 2477 2034 Size: 443 6530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 2706 3642 5872 6849 1203 Size: 1503 936 2230 977 1327 Fragments arranged by size: 2230 1503 1327 977 936 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 3416 5702 3416 Size: 2286 4687 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3167 1184 Size: 1983 4990 AlwNI CAG_nnn'CTG Cuts at: 3055 4716 6288 3055 Size: 1661 1572 3740 Fragments arranged by size: 3740 1661 1572 ApaI G_GGCC'C Cuts at: 2199 3139 2199 Size: 940 6033 ApaLI G'TGCA_C Cuts at: 5030 6186 5030 Size: 1156 5817 ApoI r'AATT_y Cuts at: 1493 3024 4282 4508 4660 6769 1493 Size: 1531 1258 226 152 2109 1697 Fragments arranged by size: 2109 1697 1531 1258 226 152 AseI AT'TA_AT Cuts at: 160 1032 2526 5527 160 Size: 872 1494 3001 1606 Fragments arranged by size: 3001 1606 1494 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2093 2159 2165 2198 3113 3872 Size: 73 71 79 66 6 33 915 759 Cuts at: 3872 4298 1870 Size: 426 4545 Fragments arranged by size: 4545 915 759 426 79 73 71 66 33 6 BamHI G'GATC_C Cuts at: 4179 4303 4670 4179 Size: 124 367 6482 Fragments arranged by size: 6482 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2217 2604 4041 4294 5749 Size: 325 217 627 430 387 1437 253 1455 Cuts at: 5749 618 Size: 1842 Fragments arranged by size: 1842 1455 1437 627 430 387 325 253 217 BanII G_rGCy'C Cuts at: 1391 2080 2139 2160 2166 2199 2232 2348 3114 Size: 689 59 21 6 33 33 116 766 Cuts at: 3114 3139 3308 4292 1391 Size: 25 169 984 4072 Fragments arranged by size: 4072 984 766 689 169 116 59 33 33 25 21 6 BbeI G_GCGC'C Cuts at: 2221 2608 4045 2221 Size: 387 1437 5149 Fragments arranged by size: 5149 1437 387 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3614 928 Size: 971 1715 4287 Fragments arranged by size: 4287 1715 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3908 3942 5164 5198 1903 Size: 34 1971 34 1222 34 3678 Fragments arranged by size: 3678 1971 1222 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3588 4865 6075 3588 Size: 1277 1210 4486 Fragments arranged by size: 4486 1277 1210 BclI T'GATC_A Cuts at: 1548 2368 1548 Size: 820 6153 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2194 3051 3485 Size: 107 122 71 631 605 522 857 434 Cuts at: 3485 4231 5584 136 Size: 746 1353 1525 Fragments arranged by size: 1525 1353 857 746 631 605 522 434 122 107 71 BglII A'GATC_T Cuts at: 2121 3075 6968 2121 Size: 954 3893 2126 Fragments arranged by size: 3893 2126 954 BlpI GC'TnA_GC Cuts at: 4390 4390 Size: 6973 Bme1580I G_kGCm'C Cuts at: 1790 2199 2494 3139 3746 5034 6190 1790 Size: 409 295 645 607 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 645 607 409 295 BmgBI CAC'GTC Cuts at: 1318 3256 1318 Size: 1938 5035 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2674 4704 5662 Size: 416 53 83 399 24 1257 2030 958 Cuts at: 5662 442 Size: 1753 Fragments arranged by size: 2030 1753 1257 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2522 2191 Size: 331 6642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2146 2561 2754 4077 5633 1239 Size: 907 415 193 1323 1556 2579 Fragments arranged by size: 2579 1556 1323 907 415 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2619 5098 5963 6261 2619 Size: 2479 865 298 3331 Fragments arranged by size: 3331 2479 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2162 3488 5636 882 Size: 617 663 1326 2148 2219 Fragments arranged by size: 2219 2148 1326 663 617 BsaAI yAC'GTr Cuts at: 493 1204 2709 3643 3835 4764 493 Size: 711 1505 934 192 929 2702 Fragments arranged by size: 2702 1505 934 929 711 192 BsaBI GATnn'nnATC Cuts at: 6967 6967 Size: 6973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2218 2605 3696 4042 Size: 53 83 186 936 685 387 1091 346 Cuts at: 4042 4780 5162 5797 275 Size: 738 382 635 1451 Fragments arranged by size: 1451 1091 936 738 685 635 387 382 346 186 83 53 BsaWI w'CCGG_w Cuts at: 2290 5406 5804 6078 6225 6636 2290 Size: 3116 398 274 147 411 2627 Fragments arranged by size: 3116 2627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 4050 4080 1178 Size: 30 165 30 758 30 1859 30 4071 Fragments arranged by size: 4071 1859 758 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 3254 3718 3752 3890 1186 Size: 1033 1035 464 34 138 4269 Fragments arranged by size: 4269 1035 1033 464 138 34 BseYI C'CCAG_C Cuts at: 2029 3154 3313 3538 6176 6672 2029 Size: 1125 159 225 2638 496 2330 Fragments arranged by size: 2638 2330 1125 496 225 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3203 1163 Size: 573 1467 4933 Fragments arranged by size: 4933 1467 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2166 2348 5034 5119 6190 2049 Size: 117 182 2686 85 1071 2832 Fragments arranged by size: 2832 2686 1071 182 117 85 BsmI GAATG_Cn' Cuts at: 3220 4484 4577 3220 Size: 1264 93 5616 Fragments arranged by size: 5616 1264 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3236 4252 3236 Size: 1016 5957 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2080 2139 2160 2166 2199 2232 Size: 399 259 31 59 21 6 33 33 Cuts at: 2232 2348 2494 3114 3139 3308 3746 4292 5034 Size: 116 146 620 25 169 438 546 742 Cuts at: 5034 5119 6190 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 546 438 399 259 169 146 116 85 59 33 33 31 25 21 6 BspEI T'CCGG_A Cuts at: 2290 6636 2290 Size: 4346 2627 BspHI T'CATG_A Cuts at: 3687 4862 6592 3687 Size: 1175 1730 4068 Fragments arranged by size: 4068 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3558 3910 4328 844 Size: 2714 352 418 3489 Fragments arranged by size: 3489 2714 418 352 BsrBI CCG'CTC Cuts at: 1394 1823 2215 4860 1394 Size: 429 392 2645 3507 Fragments arranged by size: 3507 2645 429 392 BsrDI GCAATG_nn' Cuts at: 65 1248 2701 3151 5468 5642 65 Size: 1183 1453 450 2317 174 1396 Fragments arranged by size: 2317 1453 1396 1183 450 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5617 5804 1700 Size: 110 3807 187 2869 Fragments arranged by size: 3807 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 2147 3218 1925 Size: 222 1071 5680 Fragments arranged by size: 5680 1071 222 BssSI C'ACGA_G Cuts at: 5033 6045 5033 Size: 1012 5961 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6973 BstBI TT'CG_AA Cuts at: 3819 5777 3819 Size: 1958 5015 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6973 BstXI CCAn_nnnn'nTGG Cuts at: 3835 3835 Size: 6973 BstYI r'GATC_y Cuts at: 1972 2121 2729 2769 2871 2893 3075 4179 4303 Size: 149 608 40 102 22 182 1104 124 Cuts at: 4303 4670 5055 5072 6524 6610 6622 6968 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1104 608 385 367 346 182 149 124 102 86 40 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 2466 2799 3139 3480 513 Size: 552 1401 333 340 341 4006 Fragments arranged by size: 4006 1401 552 341 340 333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3112 487 Size: 683 56 1886 4348 Fragments arranged by size: 4348 1886 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2588 2885 4092 4489 5283 5303 2061 Size: 527 297 1207 397 794 20 3731 Fragments arranged by size: 3731 1207 794 527 397 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6938 DraI TTT'AAA Cuts at: 3381 4277 3381 Size: 896 6077 DraIII CAC_nnn'GTG Cuts at: 3235 4143 3235 Size: 908 6065 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 4173 4420 Size: 54 1425 50 275 18 234 2109 247 Cuts at: 4420 5309 6938 8 Size: 889 1629 43 Fragments arranged by size: 2109 1629 1425 889 275 247 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4250 4903 1353 Size: 2897 653 3423 Fragments arranged by size: 3423 2897 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2169 4267 5560 5934 6080 387 Size: 1064 138 580 2098 1293 374 146 1280 Fragments arranged by size: 2098 1293 1280 1064 580 374 146 138 EcoICRI GAG'CTC Cuts at: 2164 2346 2164 Size: 182 6791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2561 2754 3077 4077 4259 5036 Size: 393 514 415 193 323 1000 182 777 Cuts at: 5036 5633 6420 6688 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1000 787 777 597 514 415 393 323 268 193 182 EcoNI CCTnn'n_nnAGG Cuts at: 2304 3236 2304 Size: 932 6041 EcoO109I rG'GnC_Cy Cuts at: 2098 2195 3052 3135 3756 2098 Size: 97 857 83 621 5315 Fragments arranged by size: 5315 857 621 97 83 EcoRI G'AATT_C Cuts at: 4282 4660 4282 Size: 378 6595 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 2036 2221 2479 2608 4045 6120 1678 Size: 358 185 258 129 1437 2075 2531 Fragments arranged by size: 2531 2075 1437 358 258 185 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 2496 2528 3067 Size: 32 135 32 589 32 303 32 539 Cuts at: 3067 3099 3402 3434 5614 5646 5688 5720 1373 Size: 32 303 32 2180 32 42 32 2626 Fragments arranged by size: 2626 2180 589 539 303 303 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4317 4563 669 Size: 988 281 2379 246 3079 Fragments arranged by size: 3079 2379 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6973 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KasI G'GCGC_C Cuts at: 2217 2604 4041 2217 Size: 387 1437 5149 Fragments arranged by size: 5149 1437 387 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 4572 4572 Size: 6973 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2053 3450 6087 6271 1305 Size: 748 1397 2637 184 2007 Fragments arranged by size: 2637 2007 1397 748 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4175 10 Size: 54 1425 343 2343 2808 Fragments arranged by size: 2808 2343 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2858 2864 3144 3833 4931 5290 5449 Size: 648 1692 6 280 689 1098 359 159 Cuts at: 5449 518 Size: 2042 Fragments arranged by size: 2042 1692 1098 689 648 359 280 159 6 MspA1I CmG'CkG Cuts at: 1606 2185 3651 3868 4389 5066 6214 6459 1606 Size: 579 1466 217 521 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 579 521 245 217 NarI GG'CG_CC Cuts at: 2218 2605 4042 2218 Size: 387 1437 5149 Fragments arranged by size: 5149 1437 387 NcoI C'CATG_G Cuts at: 513 1065 2799 513 Size: 552 1734 4687 Fragments arranged by size: 4687 1734 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 4331 5876 1132 Size: 97 3102 1545 2229 Fragments arranged by size: 3102 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2080 3089 3328 1348 Size: 732 1009 239 4993 Fragments arranged by size: 4993 1009 732 239 PciI A'CATG_T Cuts at: 5872 5872 Size: 6973 PflMI CCAn_nnn'nTGG Cuts at: 1108 2420 3235 1108 Size: 1312 815 4846 Fragments arranged by size: 4846 1312 815 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PmlI CAC'GTG Cuts at: 2709 3643 2709 Size: 934 6039 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3101 3133 5042 5074 6555 6587 3101 Size: 32 1909 32 1481 32 3487 Fragments arranged by size: 3487 1909 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2098 Size: 6973 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6973 PsiI TTA'TAA Cuts at: 2967 4543 2967 Size: 1576 5397 PspOMI G'GGCC_C Cuts at: 2195 3135 2195 Size: 940 6033 PspXI vC'TCGA_Gb Cuts at: 2093 2093 Size: 6973 PstI C_TGCA'G Cuts at: 830 3555 4325 830 Size: 2725 770 3478 Fragments arranged by size: 3478 2725 770 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 3868 3868 Size: 6973 SacI G_AGCT'C Cuts at: 2166 2348 2166 Size: 182 6791 SalI G'TCGA_C Cuts at: 1936 4315 1936 Size: 2379 4594 SbfI CC_TGCA'GG Cuts at: 3555 4325 3555 Size: 770 6203 ScaI AGT'ACT Cuts at: 3203 5221 6933 3203 Size: 2018 1712 3243 Fragments arranged by size: 3243 2018 1712 SexAI A'CCwGG_T Cuts at: 2305 3231 3588 2305 Size: 926 357 5690 Fragments arranged by size: 5690 926 357 SfcI C'TryA_G Cuts at: 826 1045 3551 4321 5456 6137 6328 826 Size: 219 2506 770 1135 681 191 1471 Fragments arranged by size: 2506 1471 1135 770 681 219 191 SfoI GGC'GCC Cuts at: 2219 2606 4043 2219 Size: 387 1437 5149 Fragments arranged by size: 5149 1437 387 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 2016 2200 4300 1872 Size: 144 184 2100 4545 Fragments arranged by size: 4545 2100 184 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 2165 2634 5077 5978 6240 Size: 197 926 150 72 469 2443 901 262 Cuts at: 6240 820 Size: 1553 Fragments arranged by size: 2443 1553 926 901 469 262 197 150 72 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6973 SphI G_CATG'C Cuts at: 1132 4331 1132 Size: 3199 3774 SspI AAT'ATT Cuts at: 5 52 4897 5 Size: 47 4845 2081 Fragments arranged by size: 4845 2081 47 StuI AGG'CCT Cuts at: 1517 3293 1517 Size: 1776 5197 StyI C'CwwG_G Cuts at: 513 1065 2799 4412 513 Size: 552 1734 1613 3074 Fragments arranged by size: 3074 1734 1613 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2656 5000 5017 5170 5355 5800 325 Size: 1255 1076 2344 17 153 185 445 1498 Fragments arranged by size: 2344 1498 1255 1076 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3201 5219 6931 Size: 274 80 33 51 1443 1224 2018 1712 Cuts at: 6931 96 Size: 138 Fragments arranged by size: 2018 1712 1443 1224 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4050 4082 6933 6965 4050 Size: 32 2851 32 4058 Fragments arranged by size: 4058 2851 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6973 XbaI T'CTAG_A Cuts at: 4309 4309 Size: 6973 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2941 4111 2941 Size: 1170 5803 XhoI C'TCGA_G Cuts at: 1943 2093 2165 1943 Size: 150 72 6751 Fragments arranged by size: 6751 150 72 XmaI C'CCGG_G Cuts at: 1870 2014 2198 4298 1870 Size: 144 184 2100 4545 Fragments arranged by size: 4545 2100 184 144 XmnI GAAnn'nnTTC Cuts at: 3791 5102 3791 Size: 1311 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI