(Linear) (Six-base) MAPSORT of: KIEE6762.seq Check: 5244 from: 1 to: 2268 KIEE6762.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1072 2254 2268 Size: 1072 1182 14 Fragments arranged by size: 1182 1072 14 AfeI AGC'GCT Cuts at: 0 29 472 2268 Size: 29 443 1796 Fragments arranged by size: 1796 443 29 AflIII A'CryG_T Cuts at: 0 701 1637 2268 Size: 701 936 631 Fragments arranged by size: 936 701 631 AhdI GACnn_n'nnGTC Cuts at: 0 1411 2268 Size: 1411 857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1162 2268 Size: 1162 1106 AlwNI CAG_nnn'CTG Cuts at: 0 1050 2268 Size: 1050 1218 ApaI G_GGCC'C Cuts at: 0 194 1134 2268 Size: 194 940 1134 Fragments arranged by size: 1134 940 194 ApoI r'AATT_y Cuts at: 0 1019 2268 Size: 1019 1249 AseI AT'TA_AT Cuts at: 0 521 2268 Size: 521 1747 AvaI C'yCGr_G Cuts at: 0 9 88 154 160 193 1108 1867 2268 Size: 9 79 66 6 33 915 759 401 Fragments arranged by size: 915 759 401 79 66 33 9 6 BamHI G'GATC_C Cuts at: 0 2174 2268 Size: 2174 94 BanI G'GyrC_C Cuts at: 0 212 599 2036 2268 Size: 212 387 1437 232 Fragments arranged by size: 1437 387 232 212 BanII G_rGCy'C Cuts at: 0 75 134 155 161 194 227 343 1109 Size: 75 59 21 6 33 33 116 766 Cuts at: 1109 1134 1303 2268 Size: 25 169 965 Fragments arranged by size: 965 766 169 116 75 59 33 33 25 21 6 BbeI G_GCGC'C Cuts at: 0 216 603 2040 2268 Size: 216 387 1437 228 Fragments arranged by size: 1437 387 228 216 BbsI GAAGACnn'nnnn_ Cuts at: 0 1609 2268 Size: 1609 659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1903 1937 2268 Size: 1903 34 331 Fragments arranged by size: 1903 331 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1583 2268 Size: 1583 685 BclI T'GATC_A Cuts at: 0 363 2268 Size: 363 1905 BglI GCCn_nnn'nGGC Cuts at: 0 189 1046 1480 2226 2268 Size: 189 857 434 746 42 Fragments arranged by size: 857 746 434 189 42 BglII A'GATC_T Cuts at: 0 116 1070 2268 Size: 116 954 1198 Fragments arranged by size: 1198 954 116 Bme1580I G_kGCm'C Cuts at: 0 194 489 1134 1741 2268 Size: 194 295 645 607 527 Fragments arranged by size: 645 607 527 295 194 BmgBI CAC'GTC Cuts at: 0 1251 2268 Size: 1251 1017 BmrI ACTGGGnnnn_n' Cuts at: 0 669 2268 Size: 669 1599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 186 517 2268 Size: 186 331 1751 Fragments arranged by size: 1751 331 186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 556 749 2072 2268 Size: 141 415 193 1323 196 Fragments arranged by size: 1323 415 196 193 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 614 2268 Size: 614 1654 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 1483 2268 Size: 157 1326 785 Fragments arranged by size: 1326 785 157 BsaAI yAC'GTr Cuts at: 0 704 1638 1830 2268 Size: 704 934 192 438 Fragments arranged by size: 934 704 438 192 BsaHI Gr'CG_yC Cuts at: 0 213 600 1691 2037 2268 Size: 213 387 1091 346 231 Fragments arranged by size: 1091 387 346 231 213 BsaWI w'CCGG_w Cuts at: 0 285 2268 Size: 285 1983 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 156 186 2045 2075 2268 Size: 156 30 1859 30 193 Fragments arranged by size: 1859 193 156 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 214 1249 1713 1747 1885 2268 Size: 214 1035 464 34 138 383 Fragments arranged by size: 1035 464 383 214 138 34 BseYI C'CCAG_C Cuts at: 0 24 1149 1308 1533 2268 Size: 24 1125 159 225 735 Fragments arranged by size: 1125 735 225 159 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1198 2268 Size: 1198 1070 BsiHKAI G_wGCw'C Cuts at: 0 44 161 343 2268 Size: 44 117 182 1925 Fragments arranged by size: 1925 182 117 44 BsmI GAATG_Cn' Cuts at: 0 1215 2268 Size: 1215 1053 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1231 2247 2268 Size: 1231 1016 21 Fragments arranged by size: 1231 1016 21 Bsp1286I G_dGCh'C Cuts at: 0 44 75 134 155 161 194 227 343 Size: 44 31 59 21 6 33 33 116 Cuts at: 343 489 1109 1134 1303 1741 2268 Size: 146 620 25 169 438 527 Fragments arranged by size: 620 527 438 169 146 116 59 44 33 33 31 25 21 6 BspEI T'CCGG_A Cuts at: 0 285 2268 Size: 285 1983 BspHI T'CATG_A Cuts at: 0 1682 2268 Size: 1682 586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1553 1905 2268 Size: 1553 352 363 Fragments arranged by size: 1553 363 352 BsrBI CCG'CTC Cuts at: 0 210 2268 Size: 210 2058 BsrDI GCAATG_nn' Cuts at: 0 696 1146 2268 Size: 696 450 1122 Fragments arranged by size: 1122 696 450 BssHII G'CGCG_C Cuts at: 0 142 1213 2268 Size: 142 1071 1055 Fragments arranged by size: 1071 1055 142 BstBI TT'CG_AA Cuts at: 0 1814 2268 Size: 1814 454 BstXI CCAn_nnnn'nTGG Cuts at: 0 1830 2268 Size: 1830 438 BstYI r'GATC_y Cuts at: 0 116 724 764 866 888 1070 2174 2268 Size: 116 608 40 102 22 182 1104 94 Fragments arranged by size: 1104 608 182 116 102 94 40 22 BtgI C'CryG_G Cuts at: 0 461 794 1134 1475 2268 Size: 461 333 340 341 793 Fragments arranged by size: 793 461 341 340 333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1107 2268 Size: 1107 1161 BtsI GCAGTG_nn' Cuts at: 0 56 583 880 2087 2268 Size: 56 527 297 1207 181 Fragments arranged by size: 1207 527 297 181 56 DraI TTT'AAA Cuts at: 0 1376 2268 Size: 1376 892 DraIII CAC_nnn'GTG Cuts at: 0 1230 2138 2268 Size: 1230 908 130 Fragments arranged by size: 1230 908 130 EaeI y'GGCC_r Cuts at: 0 59 2168 2268 Size: 59 2109 100 Fragments arranged by size: 2109 100 59 EarI CTCTTCn'nnn_ Cuts at: 0 2245 2268 Size: 2245 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 164 2262 2268 Size: 164 2098 6 Fragments arranged by size: 2098 164 6 EcoICRI GAG'CTC Cuts at: 0 159 341 2268 Size: 159 182 1927 Fragments arranged by size: 1927 182 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 556 749 1072 2072 2254 2268 Size: 141 415 193 323 1000 182 14 Fragments arranged by size: 1000 415 323 193 182 141 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 299 1231 2268 Size: 299 932 1037 Fragments arranged by size: 1037 932 299 EcoO109I rG'GnC_Cy Cuts at: 0 93 190 1047 1130 1751 2268 Size: 93 97 857 83 621 517 Fragments arranged by size: 857 621 517 97 93 83 HaeII r_GCGC'y Cuts at: 0 31 216 474 603 2040 2268 Size: 31 185 258 129 1437 228 Fragments arranged by size: 1437 258 228 185 129 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 156 188 491 523 1062 1094 1397 1429 Size: 156 32 303 32 539 32 303 32 Cuts at: 1429 2268 Size: 839 Fragments arranged by size: 839 539 303 303 156 32 32 32 32 KasI G'GCGC_C Cuts at: 0 212 599 2036 2268 Size: 212 387 1437 232 Fragments arranged by size: 1437 387 232 212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 48 1445 2268 Size: 48 1397 823 Fragments arranged by size: 1397 823 48 MscI TGG'CCA Cuts at: 0 2170 2268 Size: 2170 98 MslI CAynn'nnrTG Cuts at: 0 853 859 1139 1828 2268 Size: 853 6 280 689 440 Fragments arranged by size: 853 689 440 280 6 MspA1I CmG'CkG Cuts at: 0 180 1646 1863 2268 Size: 180 1466 217 405 Fragments arranged by size: 1466 405 217 180 NarI GG'CG_CC Cuts at: 0 213 600 2037 2268 Size: 213 387 1437 231 Fragments arranged by size: 1437 387 231 213 NcoI C'CATG_G Cuts at: 0 794 2268 Size: 794 1474 PasI CC'CwG_GG Cuts at: 0 75 1084 1323 2268 Size: 75 1009 239 945 Fragments arranged by size: 1009 945 239 75 PflMI CCAn_nnn'nTGG Cuts at: 0 415 1230 2268 Size: 415 815 1038 Fragments arranged by size: 1038 815 415 PmlI CAC'GTG Cuts at: 0 704 1638 2268 Size: 704 934 630 Fragments arranged by size: 934 704 630 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1096 1128 2268 Size: 1096 32 1140 Fragments arranged by size: 1140 1096 32 PpuMI rG'GwC_Cy Cuts at: 0 93 2268 Size: 93 2175 PsiI TTA'TAA Cuts at: 0 962 2268 Size: 962 1306 PspOMI G'GGCC_C Cuts at: 0 190 1130 2268 Size: 190 940 1138 Fragments arranged by size: 1138 940 190 PspXI vC'TCGA_Gb Cuts at: 0 88 2268 Size: 88 2180 PstI C_TGCA'G Cuts at: 0 1550 2268 Size: 1550 718 PvuII CAG'CTG Cuts at: 0 1863 2268 Size: 1863 405 SacI G_AGCT'C Cuts at: 0 161 343 2268 Size: 161 182 1925 Fragments arranged by size: 1925 182 161 SbfI CC_TGCA'GG Cuts at: 0 1550 2268 Size: 1550 718 ScaI AGT'ACT Cuts at: 0 1198 2268 Size: 1198 1070 SexAI A'CCwGG_T Cuts at: 0 300 1226 1583 2268 Size: 300 926 357 685 Fragments arranged by size: 926 685 357 300 SfcI C'TryA_G Cuts at: 0 1546 2268 Size: 1546 722 SfoI GGC'GCC Cuts at: 0 214 601 2038 2268 Size: 214 387 1437 230 Fragments arranged by size: 1437 387 230 214 SmaI CCC'GGG Cuts at: 0 11 195 2268 Size: 11 184 2073 Fragments arranged by size: 2073 184 11 SmlI C'TyrA_G Cuts at: 0 88 160 629 2268 Size: 88 72 469 1639 Fragments arranged by size: 1639 469 88 72 StuI AGG'CCT Cuts at: 0 1288 2268 Size: 1288 980 StyI C'CwwG_G Cuts at: 0 794 2268 Size: 794 1474 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 651 2268 Size: 651 1617 TatI w'GTAC_w Cuts at: 0 1196 2268 Size: 1196 1072 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2045 2077 2268 Size: 2045 32 191 Fragments arranged by size: 2045 191 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 936 2106 2268 Size: 936 1170 162 Fragments arranged by size: 1170 936 162 XhoI C'TCGA_G Cuts at: 0 88 160 2268 Size: 88 72 2108 Fragments arranged by size: 2108 88 72 XmaI C'CCGG_G Cuts at: 0 9 193 2268 Size: 9 184 2075 Fragments arranged by size: 2075 184 9 XmnI GAAnn'nnTTC Cuts at: 0 1786 2268 Size: 1786 482 Enzymes that do cut: AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssHII BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SbfI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AleI AloI ApaLI AscI AsiSI AvrII BaeI BbvCI BfrBI BlpI BmtI Bpu10I BsaBI BsiEI BsiWI BsrFI BsrGI BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PshAI PsrI PvuI RsrII SacII SalI SanDI SapI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI ZraI