################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbvCI BciVI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AflIII 386 ACACGT A^C[GA][CT]GT BanII 790 GAGCTC G[GA]GC[CT]^C BcgI 608 GCAAGCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 342 CCGGG CC^[GC]GG BfaI 784 CTAG C^TAG BglI 965 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 685 CTGGAG CTGGAG(16/14) BsmAI 52 GTCTC GTCTC(1/5) BsmBI 51 CGTCTC CGTCTC(1/5) BsrBI 181 CCGCTC CCGCTC(-3/-3) BsrDI 604 CATTGC GCAATG(2/0) DraII 742 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 468 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 742 GGGACCT [GA]G^G[ACGT]CC[CT] HincII 172 GTCAAC GT[CT]^[GA]AC HindII 172 GTCAAC GT[CT]^[GA]AC MaeI 784 CTAG C^TAG NciI 342 CCGGG CC^[GC]GG NspI 176 ACATGC [GA]CATG^[CT] PleI 976 GACTC GAGTC(4/5) PpuMI 742 GGGACCT [GA]G^G[AT]CC[CT] PvuII 451 CAGCTG CAG^CTG SacI 790 GAGCTC GAGCT^C SgrAI 119 CGCCGGCG C[GA]^CCGG[CT]G TfiI 921 GATTC G^A[AT]TC XcmI 278 CCATGCTCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 162 GTGCAC G^TGCAC 465 GTGCAC ApaLI 162 GTGCAC G^TGCAC 465 GTGCAC AvaII 654 GGACC G^G[AT]CC 743 GGACC BbeI 117 GGCGCC GGCGC^C 520 GGCGCC BbrPI 144 CACGTG CAC^GTG 387 CACGTG BbsI 325 GTCTTC GAAGAC(2/6) 511 GTCTTC BclI 623 TGATCA T^GATCA 773 TGATCA BpuAI 325 GTCTTC GAAGAC(2/6) 511 GTCTTC BseRI 957 CTCCTC GAGGAG(10/8) 688 GAGGAG BsiEI 225 CGACCG CG[GA][CT]^CG 306 CGACCG BsmFI 11 GGGAC GGGAC(10/14) 742 GGGAC BspMI 155 ACCTGC ACCTGC(4/8) 656 ACCTGC BstAPI 467 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 905 GCAAAAGCTGC EarI 127 CTCTTC CTCTTC(1/4) 793 CTCTTC FbaI 623 TGATCA T^GATCA 773 TGATCA HinfI 921 GATTC G^A[ACGT]TC 976 GACTC KasI 117 GGCGCC G^GCGCC 520 GGCGCC Ksp632I 127 CTCTTC CTCTTC(1/4) 793 CTCTTC MslI 615 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 661 CACCCCAGTG NarI 117 GGCGCC GG^CGCC 520 GGCGCC PmaCI 144 CACGTG CAC^GTG 387 CACGTG PmlI 144 CACGTG CAC^GTG 387 CACGTG SapI 126 GCTCTTC GCTCTTC(1/4) 792 GCTCTTC SfoI 117 GGCGCC GGC^GCC 520 GGCGCC SmlI 577 CTCAAG C^T[CT][GA]AG 586 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI BanI BbvI BlpI BmyI Bpu1102I BsaAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NdeII NgoMIV NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I