(Circular) (Six-base) MAPSORT of: pFN21AE9434.seq Check: 8738 from: 1 to: 5716 pFN21AE9434 5716 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3779 5163 5431 1632 Size: 2147 1384 268 1917 Fragments arranged by size: 2147 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2391 4615 5592 1203 Size: 1188 2224 977 1327 Fragments arranged by size: 2224 1327 1188 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 2670 1166 Size: 1504 4212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 2167 2470 3773 4929 2167 Size: 303 1303 1156 2954 Fragments arranged by size: 2954 1303 1156 303 ApoI r'AATT_y Cuts at: 1493 3025 3251 3403 5512 1493 Size: 1532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2888 2921 2888 Size: 33 5683 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2525 2809 3037 4492 Size: 325 217 627 335 403 284 228 1455 Cuts at: 4492 618 Size: 1842 Fragments arranged by size: 1842 1455 627 403 335 325 284 228 217 BanII G_rGCy'C Cuts at: 1391 2799 3035 1391 Size: 1408 236 4072 Fragments arranged by size: 4072 1408 236 BbeI G_GCGC'C Cuts at: 2126 2529 2126 Size: 403 5313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2509 928 Size: 971 424 186 4135 Fragments arranged by size: 4135 971 424 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2602 2636 3907 3941 1903 Size: 34 665 34 1271 34 3678 Fragments arranged by size: 3678 1271 665 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 2628 2778 1548 Size: 1080 150 4486 Fragments arranged by size: 4486 1080 150 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2976 4327 136 Size: 107 122 71 631 605 1304 1351 1525 Fragments arranged by size: 1525 1351 1304 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 2288 2303 2339 3133 2288 Size: 15 36 794 4871 Fragments arranged by size: 4871 794 36 15 Bme1580I G_kGCm'C Cuts at: 1790 2048 2171 2474 2814 3777 4933 1790 Size: 258 123 303 340 963 1156 2573 Fragments arranged by size: 2573 1156 963 340 303 258 123 BmgBI CAC'GTC Cuts at: 1318 2626 1318 Size: 1308 4408 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2664 2836 3447 Size: 416 53 83 399 24 1247 172 611 Cuts at: 3447 4405 442 Size: 958 1753 Fragments arranged by size: 1753 1247 958 611 416 399 172 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 4376 1239 Size: 1472 1665 2579 Fragments arranged by size: 2579 1665 1472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 2576 3841 4706 5004 2567 Size: 9 1265 865 298 3279 Fragments arranged by size: 3279 1265 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2151 2394 2577 3507 493 Size: 711 947 243 183 930 2702 Fragments arranged by size: 2702 947 930 711 243 183 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2123 2202 2526 Size: 53 83 186 936 522 68 79 324 Cuts at: 2526 3523 3905 4540 275 Size: 997 382 635 1451 Fragments arranged by size: 1451 997 936 635 522 382 324 186 83 79 68 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 1178 Size: 30 165 30 1533 30 3928 Fragments arranged by size: 3928 1533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 2953 1186 Size: 1522 245 3949 Fragments arranged by size: 3949 1522 245 BseYI C'CCAG_C Cuts at: 2316 2416 4919 5415 2316 Size: 100 2503 496 2617 Fragments arranged by size: 2617 2503 496 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2384 2500 2557 2648 2927 1163 Size: 573 345 303 116 57 91 279 3952 Fragments arranged by size: 3952 573 345 303 279 116 91 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2233 2314 3166 3927 4955 1540 Size: 275 186 232 81 852 761 1028 2301 Fragments arranged by size: 2301 1028 852 761 275 232 186 81 BsiHKAI G_wGCw'C Cuts at: 2171 2474 2568 2592 2799 3777 3862 4933 2171 Size: 303 94 24 207 978 85 1071 2954 Fragments arranged by size: 2954 1071 978 303 207 94 85 24 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 BsmBI CGTCTCn'nnnn_ Cuts at: 2062 2062 Size: 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2171 2474 2568 2592 2799 2814 Size: 399 258 123 303 94 24 207 15 Cuts at: 2814 3035 3777 3862 4933 1391 Size: 221 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 258 221 207 123 94 85 24 15 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2670 3071 844 Size: 1325 501 401 3489 Fragments arranged by size: 3489 1325 501 401 BsrBI CCG'CTC Cuts at: 1394 1823 2188 3603 1394 Size: 429 365 1415 3507 Fragments arranged by size: 3507 1415 429 365 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 4211 4385 65 Size: 1183 1360 1603 174 1396 Fragments arranged by size: 1603 1396 1360 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 2198 2221 2484 3004 4360 4547 Size: 110 315 73 23 263 520 1356 187 Cuts at: 4547 1700 Size: 2869 Fragments arranged by size: 2869 1356 520 315 263 187 110 73 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 2011 2467 2645 3776 4788 2011 Size: 456 178 1131 1012 2939 Fragments arranged by size: 2939 1131 1012 456 178 BstAPI GCAn_nnn'nTGC Cuts at: 794 2478 2916 794 Size: 1684 438 3594 Fragments arranged by size: 3594 1684 438 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2791 2848 2966 2077 Size: 714 57 118 4827 Fragments arranged by size: 4827 714 118 57 BstYI r'GATC_y Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711 Size: 1074 367 385 17 1452 86 12 346 Cuts at: 5711 1972 Size: 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2172 2325 2805 513 Size: 552 1107 153 480 3424 Fragments arranged by size: 3424 1107 552 480 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 3232 4026 4046 3232 Size: 794 20 4902 Fragments arranged by size: 4902 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DraIII CAC_nnn'GTG Cuts at: 2478 2478 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2295 2322 Size: 54 1425 50 275 18 366 99 27 Cuts at: 2322 2489 3163 4052 5681 8 Size: 167 674 889 1629 43 Fragments arranged by size: 1629 1425 889 674 366 275 167 99 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2804 3646 1353 Size: 785 666 842 3423 Fragments arranged by size: 3423 842 785 666 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2102 4303 4677 4823 387 Size: 1064 138 513 2201 374 146 1280 Fragments arranged by size: 2201 1280 1064 513 374 146 138 EcoICRI GAG'CTC Cuts at: 2797 2797 Size: 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2711 3779 4376 5163 5431 1239 Size: 393 1079 1068 597 787 268 1524 Fragments arranged by size: 1524 1079 1068 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2748 2748 Size: 5716 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2126 2132 2378 2503 2529 4863 1678 Size: 448 6 246 125 26 2334 2531 Fragments arranged by size: 2531 2334 448 246 125 26 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2940 2972 4357 4389 4431 Size: 32 135 32 1368 32 1385 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 1385 1368 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2179 3060 3306 669 Size: 988 281 241 881 246 3079 Fragments arranged by size: 3079 988 881 281 246 241 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2122 2525 2122 Size: 403 5313 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2895 4830 5014 1305 Size: 789 801 1935 184 2007 Fragments arranged by size: 2007 1935 801 789 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 2670 3674 4033 4192 518 Size: 648 1458 46 1004 359 159 2042 Fragments arranged by size: 2042 1458 1004 648 359 159 46 MspA1I CmG'CkG Cuts at: 1606 2215 2316 2338 2458 3132 3809 4957 5202 Size: 609 101 22 120 674 677 1148 245 Cuts at: 5202 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 674 609 245 120 101 22 NaeI GCC'GGC Cuts at: 2127 2200 2223 2486 2127 Size: 73 23 263 5357 Fragments arranged by size: 5357 263 73 23 NarI GG'CG_CC Cuts at: 2123 2526 2123 Size: 403 5313 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2125 2198 2221 2484 2125 Size: 73 23 263 5357 Fragments arranged by size: 5357 263 73 23 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2185 3074 4619 1132 Size: 97 956 889 1545 2229 Fragments arranged by size: 2229 1545 956 889 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PmlI CAC'GTG Cuts at: 2151 2394 2151 Size: 243 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2748 2748 Size: 5716 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2458 2458 Size: 5716 SacI G_AGCT'C Cuts at: 2799 2799 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SapI GCTCTTCn'nnn_ Cuts at: 2138 2804 2138 Size: 666 5050 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 3064 4199 4880 5071 826 Size: 219 2019 1135 681 191 1471 Fragments arranged by size: 2019 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2124 2527 2124 Size: 403 5313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 2125 1700 Size: 425 5291 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2582 2591 3820 4721 4983 820 Size: 197 926 639 9 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 639 262 197 9 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 3155 513 Size: 552 2090 3074 Fragments arranged by size: 3074 2090 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 3743 3760 3913 4098 4543 325 Size: 1255 728 1435 17 153 185 445 1498 Fragments arranged by size: 1498 1435 1255 728 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 3962 5674 Size: 274 80 33 51 1443 906 1079 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1712 1443 1079 906 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2936 2968 5676 5708 2936 Size: 32 2708 32 2944 Fragments arranged by size: 2944 2708 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2290 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI